Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

Paula, Cheila Minéia Daniel de; Geimba, Mercedes Passos; Amaral, Patrícia; Tondo, Eduardo César

doi:10.1590/s1517-83822010000400015

Cited by 25 publications

(15 citation statements)

References 24 publications

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“…For characterizing strains collected over extended periods of time, ribotyping may provide more dependable discrimination than plasmid profile analysis or antibiotic susceptibility testing since the genes encoding rRNA are chromosomally located and are not readily lost (Hinojosa-Ahumada et al, 1991). Several Shigella strains demonstrated similar PCR ribotyping banding patterns and also antibiotic resistance profile, suggesting that closely related strains were responsible for the studied foodborne shigellosis outbreaks occurred in the State of Rio Grande do Sul in Brazil (Paula et al, 2010).…”

Section: Discussionmentioning

confidence: 74%

“…Serology experiments confirmed these results. The study indicated that the several Shigella strains involved in different Shigellosis outbreaks present the same PCR-ribotyping banding pattern and also the same resistance profile, suggesting that closely related strains may have been the causative agent of the outbreaks (Paula et al, 2010). According to Navia and Gascón (Navia and Gascón, 2005), most often the cases or outbreaks of shigellosis occur due to the clonally propagation of one or a few strains.…”

Section: Ribotyping In Shigella Isolatesmentioning

confidence: 94%

“…Even though antimicrobial resistance in microorganisms could be mediated by plasmids or other transmissible DNA elements, not modifying chromosomal DNA, other molecular typing methods are required to assure the clonal relationship of the Shigella strains. PCR-ribotyping was able to correctly differentiate species of Shigella, demonstrating to be useful for foodborne outbreaks investigation (Paula et al, 2010).…”

Section: Ribotyping In Shigella Isolatesmentioning

confidence: 97%

“…Ribotyping of S. dysenteriae type 2 strains in Bangladesh showed an identical pattern of all the strains that suggested the clonal nature of the emerging strains (Talukder et al, 2006a). PCR-ribotyping has been used to characterize the Shigella isolates responsible for foodborne outbreaks occurred in the Southern Brazil (Paula et al, 2010). However, the purpose of the present review is to asses the present scenario of the ribotyping technique in the field of genome diversity of Shigella species.…”

Section: Introductionmentioning

confidence: 99%

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Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Pal¹

2014

ILNS

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Diarrhoea in developing countries is caused by an increasingly long list of bacterial, viral, and parasitic pathogens with rotavirus, Enterotoxigenic Escherichia coli, Campylobacter, Shigella, and Salmonella heading the list. Using methods to detect most of the known enteropathogens, one or more enteropathogen(s) is isolated in two-thirds of diarrhoeal illnesses in the developing world. Deoxyribonucleic acid probes have proved very useful in detecting pathogens such as enterotoxigenic (ETEC), enteroinvasive (EIEC), enteropathogenic E. coli (EPEC), and Shigella but have not yet proved to be particularly rapid or less expensive. Molecular biology has proved useful in epidemiological studies as a means of strain identification and detection of genome diversity. Since the introduction of ribonucleic acid gene restriction patterns as taxonomic tools in 1986, ribotyping has become an established method for systematics, epidemiological, ecological population and genome diversity studies of microorganisms including Shigella. The technological development culminated in the automation of ribotyping which allowed for high-throughput applications. PCR ribotyping has proved being a highly discriminatory, flexible, robust and cost-efficient routine technique which makes inter-laboratory comparison and build of ribotype databases possible, too. The aim of the present review is to determine the present status of ribotyping technique in detecting the diversity in Shigella isolates.

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Section: Discussionmentioning

confidence: 74%

Section: Ribotyping In Shigella Isolatesmentioning

confidence: 94%

Section: Ribotyping In Shigella Isolatesmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Pal¹

2014

ILNS

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“…Shigellosis is currently an important health problem around the world, occurring predominantly in children younger than five years old, mainly in developing countries [76]. In Ethiopia, as in other developing countries, shigellosis is a common cause of morbidity and mortality, particularly in children [77].…”

Section: Shigellosis In Ethiopiamentioning

confidence: 99%

Prevalence, Antibiogram and Growth Potential of Salmonella and Shigella in Ethiopia: Implications for Public Health: A Review

Girma¹

2015

Research J. of Microbiology

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This article has reviewed researches that obtained from peer-reviewed literatures on Salmonellosis and Shigellosis in different parts of Ethiopia. The prevalence of Salmonella and Shigella, the causative agent for Salmonellosis and Shigellosis, respectively, their antibiogram and growth potential were the main objectives of this review. Foodborne diseases related to unhygienic food handling practices remain a major public health problem across the globe. The problem is severe in developing countries due to limitations in securing optimal hygienic food handling practices. Data shows that an estimated 70% of cases of diarrheal diseases are associated with the consumption of foods contaminated with pathogenic microorganisms. Among these microorganisms Salmonella and Shigella are the major ones. In most studies of Antibiograms tests, Salmonella and Shigella spp. showed high resistance to the commonly used antibiotics which indicate serious problems in antimicrobial therapy globally, especially in developing countries. In challenge studies, Salmonella and Shigella spp. reached the infective dose within 4 to 24 hours of inoculation, respectively in various food samples. In this review, it is noted that these potentially pathogens are still public health problems. Therefore, there needs health education, frequent monitory and evaluation system of microbiological and antimicrobial surveillance so as to plan intervention strategies for at risk population in the area of water sanitation and hygienic food handling practice to minimize the burden posed by the diseases Salmonellosis and Shigellosis. Keywords: Antibiograms; Diarrheal diseases; Ethiopia; Foodborne diseases; Growth potential; Salmonellosis; Shigellosis. IntroductionAs far back as the documentation of human history goes, consumption of food unsafe for health and its consequences have been one of man's major health problems. They still remain to be a major public health concern globally. Food-borne diseases are known to be responsible for a large proportion of adult illnesses and deaths; more importantly, as sources of acute diarrheal diseases, they are known to claim the lives of overwhelming numbers of children every day [1].Globally, Food borne diseases remain a major public health problem. The problem is severe in developing countries due to difficulties in securing optimal hygienic food handling practices. Evidently, in developing countries, up to an estimated 70% of cases of diarrheal disease are associated with the consumption of contaminated food [1]. Transmission of enteropathogenic bacteria is affected directly or indirectly through objects contaminated with faeces. These include food and water indicating the importance of faecal-oral humanto-human transmission [2].Furthermore, acute infective diarrhoea and gastroenteritis are major causes of ill health and premature deaths in developing world due, in large part, to the lack of safe drinking water, sanitation and hygiene, as well as poorer overall health and nutritional status. According to the ...

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A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

et al. 2020

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Shigella flexneri has been a major public health problem in developing countries. This work analyzed the frequency of 16 virulence genes, the genotypic diversity, and the antimicrobial resistance profiles of 130 S. flexneri strains isolated in Brazil. The ipaH gene was found in all the 130 strains. The frequencies of the other genes were variable ial (88.5%), sigA (82.3%), iuc (74.6%), virA (73%), pic (72.3%), virF (57.7%), sat (48.5%), ipaBCD (37%), sen (36%), set1A (35.4%), sepA (30%), set1B (30%), virB (14%), icsA (10%), and ipgD (5.4%). A total of 57 (43.8%) strains were multidrug-resistant. ERIC-PCR grouped 96 of the strains into a single cluster with ≥ 70.4% of similarity, 75 of these strains presented a similarity ≥ 80.9%. PFGE grouped 120 of the strains into a single cluster with 57.4% of similarity and 82 of these strains presented a similarity ≥ 70.6%. In conclusion, the high frequency of some virulence genes reinforces the pathogenic potential of the strains studied. The high rates of MDR strains are alarming once it may lead to failure when antimicrobial treatment is necessary. Genotype techniques reveled a major cluster with high genetic similarity including S. flexneri strains from the different Brazilian states and distinct years of isolation, showing that they probably emerged from a common ancestor.

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Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

Cited by 25 publications

References 24 publications

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Prevalence, Antibiogram and Growth Potential of Salmonella and Shigella in Ethiopia: Implications for Public Health: A Review

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

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