Degradation of h-acid by free and immobilized cells of Alcaligenes latus

Ms, Usha; Mk, Sanjay; Gaddad, SM; Ct, Shivannavar

doi:10.1590/s1517-83822010000400012

Cited by 18 publications

(17 citation statements)

References 42 publications

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“…Loss of activity attributed to cell leakage can be as a result of gradual shrinkage of matrix size after batch cycle (data not provided). Immobilization matrix did not disintegrate after loss of activity and therefore disagrees with reports of activity loss linked to matrix disintegration reported elsewhere (Trevors et al, 1998;Sharanagouda and Karegoudar, 2002;Usha et al, 2010). Re-usability and stability exhibited by I. gabonensis matrix presents it as a favourable matrix for immobilization of fungal cells and subsequent use in biosorption of metals.…”

Section: Re-usability Of I Gabonensis -Immobilized T Asperellum Bhu216contrasting

confidence: 77%

“…Higher number of cycles achieved for Mn 2+ sorption can be attributed to the reduced equilibrium time compared to Al 3+ sorption. Subsequent loss of activity which has also been reported can be attributed to cell leakage (Sharanagouda and Karegoudar, 2002;Usha et al, 2010). Loss of activity attributed to cell leakage can be as a result of gradual shrinkage of matrix size after batch cycle (data not provided).…”

Section: Re-usability Of I Gabonensis -Immobilized T Asperellum Bhu216mentioning

confidence: 82%

“…Immobilization of biosorbents on suitable matrices which offers advantages including enhanced operational stability, ease of regeneration, increased effectiveness and re-usability have been used to solve the effects of these limitations (Volesky and Naja, 2005). Hydrogels such as polyacrylamide, gelatine, calcium alginate and k-carrageenan have also been used as immobilization matrices (Sharanagouda and Karegoudar, 2002;Adhinarayana et al, 2005;Ivanova et al, 2010;Usha et al, 2010;Vijayanand et al, 2012). However, there is the need to search for other low-cost materials that can serve as effective immobilization matrices.…”

Section: Introductionmentioning

confidence: 99%

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Biosorption of manganese (II) and aluminium (III) ions from aqueous solution by immobilized Trichoderma asperellum BHU216

Kareem¹,

Adewuyi²

2013

Issue 1

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Removal of Al 3+ and Mn 2+ from solution by Trichoderma asperellum BHU216 immobilized on Irvingia gabonensis matrix in a batch system was investigated. Effect of biosorption conditions contact time, pH, bead size and spore load were studied. Equilibrium isotherms and re-usability of biosorbent were also determined. The result showed that optimum sorption of Mn 2+ and Al 3+ by immobilized sorbent was achieved at 7 mm bead size and 1.6 g l -1 spore load. Optimum pH were 5 and 6 while equilibrium was attained at 120 and 180 min for Mn 2+ and Al 3+ sorption respectively. Immobilized biosorbent was used in more than 5 batch cycles with over 50% removal achieved in 3 and 5 cycles for Al 3+ and Mn 2+ sorption. Brunauer-Emmett-Teller (BET) isotherm had the best-fit than both Langmuir and Freundlich isotherms for both metals (R 2 0.98 and 0.92 for Mn 2+ and Al 3+ respectively). Manganese sorption proceeded at both surface and multi-layer levels with high sorption rates 18.88 mg l -1 and 19.72 mg l -1 , while aluminium sorption was feasible in a multi-layer process with low uptake (0.87 mg g -1 ). This study showed that immobilized T. asperellum BHU216 can effectively act as biosorbent for Mn(II) and Al(III) sorption from solution.

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Section: Re-usability Of I Gabonensis -Immobilized T Asperellum Bhu216contrasting

confidence: 77%

Section: Re-usability Of I Gabonensis -Immobilized T Asperellum Bhu216mentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Biosorption of manganese (II) and aluminium (III) ions from aqueous solution by immobilized Trichoderma asperellum BHU216

Kareem¹,

Adewuyi²

2013

Issue 1

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“…The viable cell enumeration in the CABs was performed by using a protocol 231 described previously (Usha et al, 2010). In brief, CABs was washed in saline and keep 232 submerged for 10 min (for saline soaking).…”

Section: Cell Viability Count 230mentioning

confidence: 99%

Enhanced biodegradation of naphthalene byPseudomonassp. consortium immobilized in calcium alginate beads

Dutta

Shityakov

Khalifa

et al. 2019

Preprint

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Polycyclic aromatic hydrocarbons (PAHs) belong to a large group of organic pollutant which considers as a potential health hazard to living beings. Herein, naphthalene biodegradation potential by free and immobilized Pseudomonas putida strain KD10 and Pseudomonas sp. consortium were studied. Additionally, naphthalene 1, 2-dioxygenase (nahAc) was sequenced and analyzed, which reveals two altered amino acid residues. However, the altered amino acid residues are not present in the vicinity of the active site. The gas-phase binding free energy (ΔGLondon) of the mutant variant of naphthalene 1, 2-dioxygenase was -7.10 kcal mol-1 which closely resembles the wild type variant. Naphthalene biodegradation rate by Pseudomonas putida strain KD10 was 79.12 mg L-1 day-1 and it was significantly elevated up to 123 mg L-1 day-1 by the immobilized Pseudomonas sp. consortium. The half-life (t1/2) for naphthalene biodegradation was 3.1 days with the inhibition constant (ki), substrate saturation constant (ks) and maximum specific degradation rate constant (qmax) of 1268 mg L-1, 395.5 mg L-1 and 0.65 h-1, respectively, for the Pseudomonas putida strain KD10. However, the t1/2 value was significantly reduced to 2 days along with ki, ks and qmaxvalues of 1475 mg L-1, 298.8 mg L-1 and 0.71 h-1, respectively, by the immobilized Pseudomonas sp. consortium. The GC-MS data suggest that KD10 might follow D-gluconic acid mediated meta-cleavage pathway of catechol biodegradation. It is concluded that naphthalene biodegradation performance by immobilized Pseudomonas sp. consortium was superior to free or immobilized Pseudomonas putida KD10. Microbial consortium immobilization could be a useful tool for water quality management and environmental remediation.HighlightsSuperior naphthalene biodegradation by Pseudomonas sp. consortium immobilized in calcium alginate beads.A common mutation prone amino acid stretch inside chain A of naphthalene 1, 2-dioxygenase has been identified.A new naphthalene biodegradation pathway by Pseudomonas putida strain KD10 has been proposed.

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“…Los microorganismos pueden ser inmovilizados de forma reversible o irreversible. La inmovilización reversible se lleva a cabo induciendo la adhesión de las células a superficies de matrices naturales o sintéticas como la zeolita, el poliuretano (PU), el alcohol de polivinilo (PVA), la poliacrilamida y el polietilenglicol, entre otros (Quek et al 2006, Monge et al 2008, Usha et al 2010, Silveira et al 2013, Wang et al 2015.…”

Section: Introductionunclassified

Inmovilización De Bacterias Potencialmente Degradadoras De Petróleo Crudo en Matrices Orgánicas Naturales Y Sintéticas

Reyes-Reyes¹,

Puentes-Cala

Casanova-Montes³

et al. 2018

Rev. Int. Contam. Ambie.

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Palabras clave: alginato de sodio, biomasa inmovilizada, consorcio bacteriano, biorremediación, poliuretano RESUMEN En este estudio se conformó un consorcio de bacterias potencialmente degradadores de petróleo aisladas de lodos aceitosos provenientes de un campo petrolero del nororiente colombiano. El consorcio está compuesto por cinco cepas degradadoras de crudo de rápido crecimiento, pertenecientes a los géneros Bacillus sp., Pseudomonas sp., Serratia sp., Raoultella sp. y Enterobacter sp. Con el fin de potenciar la versatilidad del consorcio bacteriano, se evaluaron diversos tipos de matrices sólidas para su inmovilización, las cuales incluyeron matrices orgánicas de origen natural (encapsulación por goteo de alginato de sodio) y sintético (adhesión en once matrices diferentes de poliuretano). La biomasa inmovilizada se cuantificó mediante recuento por microgota y peso seco. Adicionalmente, se analizó cualitativamente la distribución de los microorganismos en superficies e intersticios mediante microscopia electrónica de barrido. En todos los casos se observó algún grado de inmovilización de biomasa; sin embargo, los resultados indican que la encapsulación con alginato de sodio al 3 % y la inmovilización en las espumas de poliuretano PUF#10 y PUF#37 son más eficientes en términos de cantidad de biomasa bacteriana inmovilizada. Teniendo en cuenta su condición de biodegradables, las matrices de alginato de sodio y espuma PUF#10 se perfilan como las mejores opciones para este consorcio particular en futuros tratamientos de biorremediación.

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Degradation of h-acid by free and immobilized cells of Alcaligenes latus

Cited by 18 publications

References 42 publications

Biosorption of manganese (II) and aluminium (III) ions from aqueous solution by immobilized Trichoderma asperellum BHU216

Biosorption of manganese (II) and aluminium (III) ions from aqueous solution by immobilized Trichoderma asperellum BHU216

Enhanced biodegradation of naphthalene byPseudomonassp. consortium immobilized in calcium alginate beads

Inmovilización De Bacterias Potencialmente Degradadoras De Petróleo Crudo en Matrices Orgánicas Naturales Y Sintéticas

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