Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa

Mitov, Ivan; Strateva, Tanya; Markova, Boyka

doi:10.1590/s1517-83822010000300008

Cited by 89 publications

(77 citation statements)

References 20 publications

(7 reference statements)

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Section: Discussionsupporting

confidence: 90%

“…The frequency of ExoS toxin (63%) was in agreement with other studies done on various clinical and environmental isolates [2,30,31]. The frequency of the ExoU gene (18%) was similar to that obtained by Feltman et al [31] and Bradbury et al [32], however, it was relatively lower than that reported by Mitov et al [30] with a frequency of 30%. Moreover, the proportion of isolates (9%) having both genes was in agreement with Finnan et al [33] and Mitov et al [30], suggesting that the isolates with dual expression of both genes are increasing compared with earlier studies that showed a low number of such isolates (down to one isolate from a total of 115 isolates) [31].…”

Section: Discussionsupporting

confidence: 90%

“…The frequency of the ExoU gene (18%) was similar to that obtained by Feltman et al [31] and Bradbury et al [32], however, it was relatively lower than that reported by Mitov et al [30] with a frequency of 30%. Moreover, the proportion of isolates (9%) having both genes was in agreement with Finnan et al [33] and Mitov et al [30], suggesting that the isolates with dual expression of both genes are increasing compared with earlier studies that showed a low number of such isolates (down to one isolate from a total of 115 isolates) [31]. ExoS is known to modify bacterial invasion while ExoU is recognized for inducing cytotoxicity and is more detrimental than ExoS toxin [31,34], thus, the presence of strains producing both toxins found in this study could indicate the emergence of highly virulent strains with mixed invasive-cytotoxic genotype.…”

Section: Discussionsupporting

confidence: 86%

“…There is a controversy regarding the relation between the degree of virulence and multi-drug resistance [37]. All ExoU isolates were MDR, which is consistent with Mitov et al [30], who found a higher frequency of MDR among ExoU isolates compared with ExoS isolates. Consistent with the current finding regarding the high prevalence of fluoroquinolone resistance in ExoU isolates (100%), Wong-Beringer et al [38] found that 92% of ExoU were fluoroquinolone-resistant compared with 61% resistance in ExoS.…”

Section: Figsupporting

confidence: 79%

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Molecular Detection of the VirulentExoUGenotype ofPseudomonas aeruginosaIsolated from Infected Surgical Incisions

Hassuna

2016

Surgical Infections

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Background: Pseudomonas aeruginosa is one of the major pathogens responsible for hospital-acquired infections, which harbor a wide array of virulence factors. The main aim of this study was to determine the frequency of the virulent ExoU genotype in relation to the ExoS genotype among isolated P. aeruginosa from infected surgical incisions, followed by phylogenetic analysis. Methods: A total of 66 P. aeruginosa isolates were identified by cultural and biochemical characteristics. All isolates were tested for antimicrobial susceptibility against the following antimicrobial agents: imipenem, amikacin, gentamicin, amoxycillin, cefotaxime, cefepime, and levofloxacin. Molecular detection of the ExoS and ExoU as well as two other virulence genes was done by polymerase chain reaction (PCR). Sequencing of ExoU gene and phylogenetic analysis was performed.Results: Approximately 81% of the isolated P. aeruginosa were multi-drug resistant. The ExoS genotype was more prevalent (63%) among the isolates than the ExoU genotype (18%), with 9% of the isolates possessing both toxins. LasB and AprA were detected in 63.6% and 27.2% of the isolates, respectively. An association was observed between the number of virulence genes and the presence of multi-drug resistance. All the ExoU were multi-drug resistant (MDR), whereas 71% of the ExoS were MDR. Phylogenetic analysis of ExoU gene showed a 99% similarity with four different strains. Conclusion: Despite the greater frequency of the ExoS genotype, the presence of the virulent MDR ExoU genotype isolates from surgical site infections is an alarming sign requiring further intervention and investigations.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

Section: Figsupporting

confidence: 79%

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Molecular Detection of the VirulentExoUGenotype ofPseudomonas aeruginosaIsolated from Infected Surgical Incisions

Hassuna

2016

Surgical Infections

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“…Previous studies have shown a high prevalence of the lasB gene in P. aeruginosa, irrespective of their isolation origin [5,6,24]. The prevalence of ExoS observed in this study shows that the strain of Pseudomonas aeruginosa of animal origin could be involved in tissue destruction; ExoS gene is involved in lung infection and may be important for bacterial dissemination [6,7,24].…”

Section: Discussionmentioning

confidence: 50%

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Benie

Dadié

Guessennd

et al. 2017

JBMOA

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Exploring the potential human pathogenic bacteria in selected ready‐to‐eat leafy greens sold in Dhaka City, Bangladesh: Estimation of bacterial load and incidence

Ferdous,

Sultana,

Hossain

et al. 2023

Food Science & Nutrition

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This study was designed to investigate the presence of potential human pathogenic bacteria, bacterial load, and their incidence in ready‐to‐eat leafy greens viz., coriander, lettuce, and mint leaves sold at diverse marketplaces in Dhaka City. Multiple identification methods including cultural, morphological, biochemical, and molecular analysis were employed in the Plant Pathology Laboratory of Sher‐e‐Bangla Agricultural University to identify the human pathogenic bacteria. In molecular analysis, the DNA samples were put through PCR using bacterial primer 27F: AGAGTTTGATCMTGGCTGAG and universal primer 1942R: CGGTTACCTTGTTACGACTT. Initially, nine different bacterial genera viz. Bacillus, Escherichia, Pseudomonas, Neisseria, Klebsiella, Enterobacter, Shigella, Vibrio, and Staphylococcus were detected, and their incidence was 93%, 67%, 44%, 30%, 26%, 26%, 11%, 7%, and 7% respectively. A total of twelve bacteria have been identified from these genera out of which 7 bacteria viz. Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Staphylococcus aureus, and Shigella spp., were reported as human pathogenic bacteria in several pieces of literature. The highest colony‐forming units per gram were shown in mint (4.27 ± 2.35 × 109) followed by lettuce (2.87 ± 0.76 × 109) and coriander (2.43 ± 1.32 × 109). Considering marketplaces, the highest colony‐forming units per gram were observed in the samples of street markets (5.0 ± 1.72 × 109) and the lowest was in supermarkets (1.87 ± 0.46 × 109) followed by local markets (2.7 ± 0.91 × 109). All the leafy green samples crossed the acceptable level of bacterial load (106 CFU/g). The findings of the study highlight the urgency for improved food safety protocols in their production and distribution in Dhaka city.

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Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa

Abstract: The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160)

Cited by 89 publications

References 20 publications

Molecular Detection of the VirulentExoUGenotype ofPseudomonas aeruginosaIsolated from Infected Surgical Incisions

Molecular Detection of the VirulentExoUGenotype ofPseudomonas aeruginosaIsolated from Infected Surgical Incisions

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Exploring the potential human pathogenic bacteria in selected ready‐to‐eat leafy greens sold in Dhaka City, Bangladesh: Estimation of bacterial load and incidence

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