Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191

Abstract

This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25 o C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25 o C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.

Mycoparasitic nature of Bionectria sp. strain 6.21

“…The chitinase activity was measured according to the method described by Fleuri et al (2009), using colloidal chitin. A unit of the enzyme activity was defined as 1 µmol of N-acetylglucosamine formed in the conditions of this study.…”

Optimization of the Chitinase Production by Different <i>Metarhizium anisopliae</i> Strains under Solid-State Fermentation with Silkworm Chrysalis as Substrate Using CCRD

“…On the other hand, the use of low cost substrates has a significant advantage under economic view, reducing the production costs in the industry. Many works showing chitinase production have used colloidal chitin or crab and shrimp shell powder [22][23][24][25] as the main substrate/carbon source. Chitinase production using arthropods cuticle was also demonstrated by Da [4].…”

Enhanced chitinase production by <i>Chitinolyticbacter meiyuanensis</i> SYBC-H1 using staged pH control

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None of the authors of this manuscript has any financial or personal relationship with other people or organizations that could inappropriately influence their work.to their wide range of biotechnological applications, such as shellfish waste management (Nakagawa et al, 2011), generation of fungal protoplasts (Fleuri et al, 2009), production of single cell protein (Wang et al, 2006), production of N-acetyl-b-D-glucosamine (GlcNAc) and derivatives (Lin et al, 2009) and bio-control of fungal plant pathogens (Joo, 2005).Despite the potential commercial benefits, the application of chitinases has been limited by low productivity (Liu et al, 2013), which is of particular relevance to the production by the enzymatic degradation of chitin. Thus, increasing the levels of chitinase production is a key to improving GlcNAc production on an industrial scale.

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“…to their wide range of biotechnological applications, such as shellfish waste management (Nakagawa et al, 2011), generation of fungal protoplasts (Fleuri et al, 2009), production of single cell protein (Wang et al, 2006), production of N-acetyl-b-D-glucosamine (GlcNAc) and derivatives (Lin et al, 2009) and bio-control of fungal plant pathogens (Joo, 2005).…”