Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Abstract

Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some highaccumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying interspecific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.

Maize endophytic bacteria as mineral phosphate solubilizers

“…The analysis of variance for each test was made using the Sisvar 5.3 program (Ferreira, 2010). Significant differences were analyzed by the F test (P ≤ 0.05), and the means were compared using the Scott-Knott test at 5% probability.…”

“…The gluconic acid production was prevalent in all the PSB studied, and species of the genus Bacillus were the most efficient phosphate solubilizers. Bacillus species are easily isolated from maize, suggesting an evolutionary relationship between these organisms (Figueiredo et al, 2009). This indicates that Bacillus species may be used for selecting the most adapted and efficient phosphate solubilizers from maize.…”

“…The PCR conditions used were according to that described in Figueiredo et al (2009). The amplicons were purified using the QIAquick gel extraction kit (Qiagen, Hilden, Germany).…”

Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy

“…Several fungal (Li et al, 2010;Qiu et al, 2010) and even bacterial (Andreote et al, 2009;Figueiredo et al, 2009) endophytes colonize plants. Endophytes can also protect the plant from many biotic and abiotic threats (Azevedo et al, 2000).…”

Isolation of Mexican Bacillus Species and Their Effects in Promoting Growth of Chili Pepper (Capsicum annuum L. cv Jalapeño)

“…subtilis and B. pumilus are the most frequently isolated rhizobacteria followed by B. licheniformis, B. cereus and B. amiloliquefascens [15]. Here, in order to identify the rhizospheric bacterial strains, we amplified the 16S rDNA obtaining amplicons of *1.5 kbp, which were sequenced and compared with GenBank data in NCBI.…”