A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens

Paiva, Jacqueline Boldrin de; Filho, Rafael Antonio Casarin Penha; Arguello, Yuli Melisa Sierra; Berchieri, Ângelo; Lemos, Manuel Victor Franco; Barrow, Paul

doi:10.1590/s1517-83822009000300012

Cited by 13 publications

(10 citation statements)

References 49 publications

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“…Currently, live vaccines (LV) against SG are frequently used in the fields. The attenuated strains are able to colonize internal organs, without clinical symptoms, however they induce an efficient immune response (DE PAIVA et al, 2009;LEE et al, 2005;SHEHATA et al, 2013). The use of LV prepared with attenuated strains of SG has showed good immunogenicity and capacity to induce a strong cellular and humoral immune response in experimental models (MATSUDA et al, 2011a;2011b;PENHA FILHO et al, 2012).…”

Section: Palavras-chavementioning

confidence: 99%

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Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

et al. 2018

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Salmonella enterica serovar Gallinarum biovar Gallinarum (SG) is a host-specific bacteria that causes the fowl typhoid (FT). This disease is highly pathogenic to commercial chickens, specially brown layers and breeders, causing acute septicemia followed by high morbidity and mortality. Vaccination is extensively adopted in the fields as a biosafety tool for prevention of isolated infections and outbreaks in commercial poultry flocks. The present study evaluated the use of an attenuated SG with deletions on genes cobS and cbiA (SGΔcobSΔcbiA) as a live vaccine, using vaccination schemes adjusted for field conditions. To this end, brown layers were used in two different experiments, to evaluate the long-term protection, necessary in the fields. The vaccination scheme on the first experiment consisted of two doses, the first at 4 th week-of-age and the booster dose at 8 th week-of-age with challenge at 16 th week-of-age with wild SG strain. On the second experiment, the vaccination was carried out by different routes using three doses of the live vaccine, at 4 th , 8 th and 12 th weeks-of-age, and the challenge was done at 20 th weeks-of-age. After the challenge, the mortality was recorded during 28 days, and the egg production (experiment 2) was evaluated and compared with the group of unvaccinated layers. In both experiments, the mortality was significantly reduced, and the egg production was not affected in vaccinated layer-hens. In summary, this study shows the efficacy and the protection of different vaccination schemes against FT that can be applied under field conditions in commercial poultry farms.

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Section: Palavras-chavementioning

confidence: 99%

“…Mutant strain of SG with deleted genes cobS and cbiA (SGΔcobSΔcbiA), attenuated for commercial chickens, was constructed according to the previously described (DE PAIVA et al, 2009) and used LV. The construction and use of the mutant strain was approved by the National Technical Committee of Biosecurity (Protocol no.…”

Section: Inoculums and Vaccinesmentioning

confidence: 99%

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

et al. 2018

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“…The live vaccine (LV) consisted of the attenuated SGΔcobSΔcbiA (de Paiva et al, 2009;Penha Filho et al, 2010), resistant to kanamycin. An invasive highly pathogenic SG strain (SG 287/91), kindly donated by Prof. Paul Barrow (University of Nottingham, UK) was used to challenge birds.…”

Section: Bacterial Strains and Vaccinesmentioning

confidence: 99%

“…The strain SGΔcobSΔcbiA is a mutant strain of SG 287/91, with deletion on genes cobS and cbiA, previously shown to be attenuated and capable to colonize internal organs, in reduced numbers in comparison with wild SG strain (de Paiva et al, 2009). Previous studies have also shown that this strain is immunogenic and one vaccine dose elicited protection against SG and cross protection against S. Enteritidis (Penha Filho et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of protective immune response against fowl typhoid in chickens vaccinated with the attenuated strain Salmonella Gallinarum Δ cob SΔ cbi A

Filho

Díaz

Medina

et al. 2016

Research in Veterinary Science

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Salmonella enterica serovar Gallinarum biovar Gallinarum (SG) causes fowl typhoid in chickens, a septicemic infection which results in high mortality rates. This disease causes high economic impact to the poultry industry worldwide because of the mortality or elimination of positive flocks to control bacterial dissemination. Live vaccines are used in the fields, however the characterization of immune mechanisms important for protection are being studied to improve the efficacy of vaccination schemes. In this study, we evaluated the immune response in brown layer-hens, vaccinated or not, during the most critical period of infection. Cellular and humoral immunity were extensively evaluated until 7 days post-infection (DPI), by flow cytometry and ELISA, respectively. Furthermore, we evaluated the expression of important pro-inflammatory cytokines after infection of bone marrow derived macrophages (BMDMs) with the live attenuated SG vaccine and with the wild SG strain. The results showed an increasing production of IgG and IgM during the first week post-infection, in vaccinated layer-hens, which was absent in unvaccinated birds. The population of CD8(+)CD44(+) and CD4(+)CD44(+) T cells in spleen and cecal tonsils constantly decreased in unvaccinated birds in comparison with vaccinated layers. The expression of IFN-γ and TNF-α in BMDMs was induced by both SG strains (attenuated and wild) at similar levels (p>0.05). Vaccination with live SG vaccine reduced systemic infection by challenge strain of SG and prevented the mortality rate of 85% that occurred in unvaccinated layer-hens during 30 dpi. Furthermore, the immunization enhanced the proliferation of effector CD4(+) and CD8(+) T cells after challenge.

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“…The description of the construction of the attenuated nalidixic acid-resistant strain of Salmonella Gallinarum, carrying deletion on cobS and cbiA genes (SGDcobSDcbiA) was described by Paiva et al (2009b). A pathogenic strain of Salmonella Gallinarum resistant to nalidixic acid was used to challenge the brown layers and a pathogenic strain of Salmonella Enteritidis resistant to nalidixic acid and spectinomycin was used to challenge the white-egg layers.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Safety and efficacy of a salmonella gallinarum ΔcobSΔcbiA strain with potential to prevent chicken infections by salmonella gallinarum and salmonella enteritidis

Paiva

Filho

Moura

et al. 2012

Rev. Bras. Cienc. Avic.

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New vaccine design techniques have allowed the development of effective vaccine strains against Salmonella infections in which the risks of reversion to the wild type and virulence is null. The mutant strain Salmonella Gallinarum ΔcobSΔcbiA was previously shown to be avirulent in chickens. In this study, this strain was tested as a vaccine against Salmonella Gallinarum (SG) and S. Enteritidis (SE) infections, and its protection levels, safety and possible risks of reversion to virulence after vaccination of layers were evaluated. Birds were vaccinated at five days of age or at five and 25 days of age. At 45 days of age, brown and white layers were challenged with SG and SE wild strains, respectively. Two assays to test the possibility of reversion to virulence were performed. Five successive bacterial passages in brown layers were carried out in the first assay. In the second assay, brown layers received a ten-fold concentrated inoculum of the SGΔcobSΔcbiA strain and were evaluated for clinical signs and mortality. In both experiments, no birds that received the inoculation of the attenuated strain died. Additionally, the use of the mutant strain as a vaccine provided good protection levels against both challenge strains.

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A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens

Cited by 13 publications

References 49 publications

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

Evaluation of protective immune response against fowl typhoid in chickens vaccinated with the attenuated strain Salmonella Gallinarum Δ cob SΔ cbi A

Safety and efficacy of a salmonella gallinarum ΔcobSΔcbiA strain with potential to prevent chicken infections by salmonella gallinarum and salmonella enteritidis

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