Assessing the diversity of bacterial communities associated with plants

Andreote, Fernando Dini; Azevedo, João Lúcio de; Araújo, Welington Luiz

doi:10.1590/s1517-83822009000300001

Cited by 84 publications

(57 citation statements)

References 76 publications

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“…Most of the research on this topic has focused on the functional roles of single microbial groups (e.g., specific species or organisms from the same genera) associated with plants, mostly because of methodological limitations to assess non-culturable microbial groups (Amann et al, 1995;Andreote et al, 2009). Examples of these inferences are related to specific microbial groups able to promote plant growth, such as nitrogen-fixing bacteria (Raymond et al, 2004) and mycorrhiza-forming fungi (Chagnon et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Exploring interactions of plant microbiomes

Andreote

Gumiere

Durrer

2014

Sci. agric. (Piracicaba, Braz.)

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141

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A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance nutrient acquisition, and affect stress tolerance. In this review, we first summarize features of microbial communities that compose the plant microbiome and further present a series of studies describing the underpinning factors that shape the phylogenetic and functional plantassociated communities. We advocate the idea that understanding the mechanisms by which plants select and interact with their microbiomes may have a direct effect on plant development and health, and further lead to the establishment of novel microbiome-driven strategies, that can cope with the development of a more sustainable agriculture.

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Section: Introductionmentioning

confidence: 99%

Exploring interactions of plant microbiomes

Andreote

Gumiere

Durrer

2014

Sci. agric. (Piracicaba, Braz.)

Self Cite

141

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“…34 For this reason, many studies aiming to quantify the effects of transgenic plants on microbial-associated communities have used this instrument. 37 Community composition as well as capabilities of the endophytes for solubilisation of phosphate, fixation of atmospheric nitrogen, production of antifungal metabolites, and production of IAA were compared between Bt and non-Bt maize genotypes for the pre-and post-flowering stages.…”

Section: Statistical Analysesmentioning

confidence: 99%

Community composition and functions of endophytic bacteria of Bt maize

et al. 2018

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We investigated the potential effects of genetic modification of Bt maize on the community composition and functions of bacterial endophytes associated with transgenic maize (Bt MON 810) in comparison with its isogenic parental line at two developmental stages. Bacterial isolates were obtained from transgenic (Bt) and non-transgenic (non-Bt) maize at 50- and 90-day-old developmental stages. Isolated bacterial endophytes were screened for their capabilities in phosphate solubilisation, nitrogen fixation, production of antifungal metabolites and production of indole acetic acid. After molecular identification, 60 isolates were obtained and clustered into 19 and 18 operational taxonomic units from 50- and 90-day-old maize, respectively. The isolates belonged to the genera Bacillus, Pantoea, Serratia, Yersinia, Enterobacter, Pseudomonas, Acinetobacter and Stenotrophomonas. Functional attributes and diversity of the isolated endophytes at both developmental stages were not significantly different for both maize varieties. However, functional attributes were significantly affected by plant growth stage. Isolates from younger plants were more efficient producers of indole acetic acid, but exhibited little or no capabilities for nitrogen fixation, phosphate solubilisation and antifungal activity in both maize genotypes. Based on these outcomes, Bt modification in maize does not seem to affect the community composition or functional attributes of bacterial endophytes. Significance: • Bt modification in maize does not affect the ecological guild or functional attributes of cultivable bacterial endophytes.

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“…The study of bacterial diversity can be performed by the BOX-PCR technique, which uses the DNA of bacteria cultured in vitro. However, bacterial culture provides limited information, because culture medium does not accurately reproduce the different ecological niches in the laboratory environment [38,39].…”

Section: Degradation Stage and Association Regionmentioning

confidence: 99%

Diazotrophic Bacterial Community of Degraded Pastures

Oliveira

Figueredo

Diniz

et al. 2017

Applied and Environmental Soil Science

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Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady's table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nif H gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments.

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Assessing the diversity of bacterial communities associated with plants

Cited by 84 publications

References 76 publications

Exploring interactions of plant microbiomes

Exploring interactions of plant microbiomes

Community composition and functions of endophytic bacteria of Bt maize

Diazotrophic Bacterial Community of Degraded Pastures

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