Serum antileptospiral agglutinins in freshwater turtles from Southern Brazil

Silva, Éverton F.; Seyffert, Núbia; Cerqueira, Gustavo M.; Leihs, Karl P.; Athanazio, Daniel Abensur; Valente, Ana Luísa; Dellagostin, Odir A.; Brod, Claudiomar Soares

doi:10.1590/s1517-83822009000200003

Cited by 15 publications

(18 citation statements)

References 10 publications

(11 reference statements)

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“…In Brazil, Silva et al (2009) found that Trachemys dorbigny from urban lakes in the municipality of Pelotas, Southern Brazil, were also reactive for the Tarassovi serogroup; however, the largest number of animals were reactive for the Bataviae serogroup, which contrasts with the results of the present study.…”

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confidence: 99%

Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity

Rocha

Baia

Monteiro

et al. 2019

SCA

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Leptospirosis is a zoonosis of worldwide distribution in which the agent can infect several animal species and accidentally humans. In view of the limited number of studies on anti-Leptospira antibodies in wild animal species, especially those living in aquatic environments, we sought in this study to investigate the presence of these antibodies in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity in the Rodrigues Alves Botanical Garden-Amazon Zoobotanical Park, located in Belém, Pará State, Brazil. Serum samples were collected from 31 turtles, and identification of anti-Leptospira antibodies was performed using the microscopic agglutination test, using a collection of 31 live antigens which represent 19 serogroups of Leptospira. Among the analyzed samples, 54.83% (17/31) were observed to be reactive, and co-agglutination was detected in a further six samples which were not accounted for in the frequency of serogroups. The most frequently detected serogroups were Tarassovi 72.72% (8/11), Celledoni 18.18% (2/11), and Pomona 9.09% (1/11)], with titers ranging from 200 to 400, being this the first study to report infection of these serogroups in this species of chelonios. The animals were kept in water tanks, which probably favored the transport of the agent and allowed its transmission to the spot-legged turtle. We thus confirmed presence of anti-Leptospira antibodies in chelonians maintained in the Rodrigues Alves Botanical Garden.

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confidence: 99%

Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity

Rocha

Baia

Monteiro

et al. 2019

SCA

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“…MAT has been used as serological test for Leptospira spp. diagnostic (OIE, 2016) in testudines (Andrews et al, 1965;Esteves et al, 2005;Glosser et al, 1974;Silva et al, 2009Silva et al, , 2010Grimm et al, 2015). In order to analyze the sensitivity and specificity of MAT in these study, PCR was used as the gold standard diagnostic technique.…”

Section: Evaluation Of Mat Sensitivity and Specificitymentioning

confidence: 99%

“…Studies performed in Emydoidea blandingii (Blanding's turtles) have found anti-Leptospira antibodies in 100% (34/34) (Mitchell et al, 2015) and 93.5% (29/31) (Grimm et al, 2015) of the samples. In Trachemys dorbigny (Black-bellied Slider) and Phrynops hilarii (Hilaire's Side-necked Turtle), 27.5% (11/40) of the animals were positive (Silva et al, 2009). Glosser et al (1974) described that 91.7% (44/48) of the samples from Sternothaerus odoratus (Stinkpot turtle) and Trachemys scripta elegans (Red-eared Slider) were positive.…”

Section: Leptospira Microagglutination Testmentioning

confidence: 99%

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Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

et al. 2016

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Leptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.

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“…This might be the reason why Semaranga and Bataviae serogroups had the highest titers (3200). Bataviae has also been detected in Trachemys dorbigni (D'Orbigny's slider) (SILVA et al, 2009).…”

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Presence of anti-Leptospira spp. antibodies in captive yellow-spotted river turtles (Podocnemis unifilis) in the Eastern Amazon

et al. 2020

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We describe anti-Leptospira spp. agglutinin in yellow-spotted river turtles (Podocnemis unifilis)in the Amazon region. Ninety-eight serum samples from individuals housed at the Bosque Rodrigues Alves Zoobotanical Garden of Amazonia, Belém, PA, Brazil, were subject to the microscopic agglutination test (MAT) using 19 different Leptospira spp. antigen serogroups. Thirty-four of the 98 samples (35%) were reactive, with titers ranging from 100 to 3200, and eight 8 reactive samples (23.5%) co-agglutinated under two or more serovars.The most common serogroup was Hebdomadis (26.9%, 7/26), followed by Semaranga (23%, 6/26), Shermani (19.2%, 5/26), Djasiman (11.5%, 3/26), and Australis (7.7%, 2/26); Bataviae, Javanica, and Sejroewere represented by a single sample each (3.9%). The presence of turtles reactive to anti-Leptospira spp. antibodies implies exposure to the pathogen.

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Serum antileptospiral agglutinins in freshwater turtles from Southern Brazil

Cited by 15 publications

References 10 publications

Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity

Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity

Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

Presence of anti-Leptospira spp. antibodies in captive yellow-spotted river turtles (Podocnemis unifilis) in the Eastern Amazon

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