Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)

Maheshwar, P. K.; Moharram, S. Ahmed; Janardhana, G. R.

doi:10.1590/s1517-83822009000100023

Cited by 24 publications

(12 citation statements)

References 13 publications

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“…Of the five species of Fusarium isolated in this study, four, namely F. proliferatum, F. verticillioides, F. chlamydosporum and Fusarium sp., were shown in this mycological study to be mycotoxigenic producing FB, ZEA, DON and T-2 on YES. F. proliferatum and F. verticillioides, which were shown herein as producers of FB1 and FB2, are not only important toxin-producing fungi associated with maize worldwide (Dutton 1996;Kpodo et al 2000;Marasas 2001), but have been isolated from other food commodities (Reddy et al 2006) including rice (Pitt and Hocking 1997;Pacin et al 2002;Makun et al 2007;Maheshwar et al 2009). In fact, Park et al (2005) found F. proliferatum as the most frequent Fusarium spp.…”

Section: Discussionmentioning

confidence: 86%

Incidence, Phylogeny and Mycotoxigenic Potentials of Fungi Isolated From Rice in Niger State, Nigeria

Makun

Dutton

Njobeh

et al. 2011

Journal of Food Safety

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The study reports on the natural occurrence of fungi in 21 samples of field (10), stored (6) and marketed (5) rice (Oryza sativa L.) collected from Niger State, Nigeria. Fungal isolates were primarily identified based on morphological characteristics, while representative isolates were characterized genetically. An evolutionary tree was constructed from the resulting sequences of the isolated fungi. The toxigenic potentials of some of the isolated fungi were also determined. A total of 357 fungal isolates of nine genera including Aspergillus, Fusarium, Sarocladium, Acremonium, Curvularia Botryosphaeria, Penicillium Alternaria and Ascomycota in decreasing order of predominance were identified. The most frequent fungal contaminants of the rice samples were A. flavus, A. fumigates, A. niger, A. parasiticus and F. proliferatum. All strains of A. flavus (aflatoxins B1 and B2), A. parasiticus (aflatoxins B1, B2, G1 and G2), A. ochraceus (ochratoxin A), F. proliferatum and F. verticillioides (fumonisins B1 and B2) tested, were excellent producers of their respective mycotoxins. Patulin was produced by A. terreus, whereas deoxynivalenol, zearalenone and T‐2 toxin were produced by F. chlamydosporum and other Fusarium spp. The increased prevalence of toxigenic fungi in rice, a highly consumed food grain in Nigeria, poses serious health concerns to the general public. PRACTICAL APPLICATIONS This study investigated the natural fungal occurrence of rice grown in Nigeria using polymerase chain reaction‐based techniques as well as the toxigenic potentials of some of the identified fungi. The resultant fungal profile of rice, gene sequences of the fungi detected in the survey, which were deposited in the GenBank and the constructed evolutionary tree, will serve as reference data for the incidence of fungal species in rice and will help to evaluate the safety of Nigerian rice. It could be used in developing and predicting the degree of mycotoxin contamination in rice from Nigeria for effective mycotoxin control. The toxigenic fungi acquired from the work will be excellent microbial sources for production of mycotoxin standards namely aflatoxins BI, B2, G1 and G2, ochratoxin A and fumonisin B1 and B2.

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Section: Discussionmentioning

confidence: 86%

Incidence, Phylogeny and Mycotoxigenic Potentials of Fungi Isolated From Rice in Niger State, Nigeria

Makun

Dutton

Njobeh

et al. 2011

Journal of Food Safety

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“…and several related species contaminate and produce Fumonisins in rice and other stored food products like corn, wheat, asparagus, cowpeas, maize, sorghum, millet, farro, black tea, beer etc. (Kushiro et al 2008(Kushiro et al , 2009Maheshwar et al 2009;Karthikeyan et al 2011;Seefelder et al 2002). Fumonisins are a series of structurally related sphingosine analog toxins (Shier 1992), the most abundant and one of the most active members of this series is Fumonisin B1 (FB1) (Abbas et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Genetic diversity of fumonisin producing Fusarium isolates from rice using PCR-RFLP of IGS-rDNA region

et al. 2018

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Nayak S, Dhua U, Chhotaray A, Samanta S, Sengupta C. 2018. Short Communication: Genetic diversity of fumonisin producing Fusarium isolates from rice using PCR-RFLP of IGS-rDNA region. Biodiversitas 19: 571-576. Fusarium verticillioides (Sacc.) and related species produce carcinogenic mycotoxin known as Fumonisins in several agricultural crops including rice. However, this principal food crop has been infected by genetically diverse Fusarium species. Odisha belongs to the coastal part of India and many popular rice varieties are in the food chain in this region. Many Fusarium species producing fumonisins have been found to be associated with these rice varieties. Hence, the genetic diversity of twenty eight Fumonisin producers and non producers of Fusarium pathogens in this region was carried out in the current study. The IGS regions of 28 Fusarium isolates (both fumonisin producing and non producing) were amplified and the PCR products were restriction digested with ECoRI and HhaI. The digested products were separated on PAGE and bands were visualized by Silver Nitrate Staining. The 28 isolates could be separated into 14 IGS haplotypes. The lowest similarity was detected to be of 33% between F40 and F47. A group containing 14 isolates represented the biggest haplotypes. The isolates in which the FUM gene had not been detected (fumonisin non producer) were in a separate group having 90% similarity with each other and placed consistently in separate branch from others. Presence of unique band for this group was observed at 1650bp where as absence of specific bands was observed at 380bp and 300bp. The result of this study indicated a high degree of genetic variation among 28 Fusarium isolates. PCR RFLP of IGS region was also found to be useful for diversity study in Fusarium.

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“…Mycotoxin contamination in foods and feedstuffs poses serious health hazard to animals and humans (Mokhles et al, 2007;Iheshiulor et al, 2011). Mycotoxins are commonly produced by species of Aspergillus, Penicillium and Fusarium (Chandra and Sarbhoy, 1997;Masheshwar et al, 2009). Several strategies are used at controlling fungal growth and the mycotoxin biosynthesis in seeds, grains and feedstuff by chemical treatments, and food preservatives, by physical and biological methods.…”

Section: Introductionmentioning

confidence: 99%

Control of Toxigenic Fungi and Mycotoxins with Phytochemicals: Potentials and Challenges

Anjorin¹,

Salako²,

Makun³

2013

Mycotoxin and Food Safety in Developing Countries

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Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)

Cited by 24 publications

References 13 publications

Incidence, Phylogeny and Mycotoxigenic Potentials of Fungi Isolated From Rice in Niger State, Nigeria

Incidence, Phylogeny and Mycotoxigenic Potentials of Fungi Isolated From Rice in Niger State, Nigeria

Short Communication: Genetic diversity of fumonisin producing Fusarium isolates from rice using PCR-RFLP of IGS-rDNA region

Control of Toxigenic Fungi and Mycotoxins with Phytochemicals: Potentials and Challenges

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