A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance

Sotero-Martins, Adriana; Jesus, Michele Silva de; Lacerda, Michele; Moreira, Josino Costa; Filgueiras, Ana Luzia Lauria; Barrocas, Paulo Rubens Guimarães

doi:10.1590/s1517-83822008000200020

Cited by 30 publications

(13 citation statements)

References 24 publications

(25 reference statements)

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“…A clear DNA band (350 bp) was obtained with primers MR3/MR2 that are specific to Tn5036-like determinant whereas no PCR products were obtained by using the primers of the other determinants. These results are compatible with those who used the merA gene as a molecular marker to follow the assortment of mercury resistant bacterial population under the pressure of mercury toxicity in aerobic and anaerobic environments (Felske et al, 2003;Simbahan et al, 2005;Sotero-Martins et al, 2008).…”

Section: Effect Of Mercury Stress On a Mercury Reducing Biofilmsupporting

confidence: 90%

The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains

Essa¹

2012

Afr. J. Microbiol. Res.

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Active resistance to the mercuric ion is widely distributed in environmental microbes and results from the action of mercuric reductase. Five mercury resistant bacteria: Escherichia coli Z1, Escherichia coli Z3, Pseudomonas putida Z2, Serratia marcescens Z4 and Xanthomonas sp. Z5 were isolated and identified from sludge sample. The presence of mercury resistance determinants was screened by PCR using merA-specific primers. Based on the analysis of merA amplicons, high similarity was recorded between the merA region of the strains P. putida Z2 and Xanthomonas sp. Z5 with those of Tn5053; while the merA of E. coli Z1 was analogous to those of Tn21. In case of the bacterial strains E. coli Z3 and S. marcescens Z4 a great matching was obtained between their merA and those of Tn5036. The effect of mercury stress upon the structure of mercury reducing biofilm at the species level and the type of mercury resistance determinants was studied in a continuous bioreactor. Community analysis suggested that the bacterial strain E. coli Z3 containing Tn5036-like determinant is the well adapted strain that tolerated elevated levels of mercury whereas the other strains showed a less fitness under these extreme conditions.

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Section: Effect Of Mercury Stress On a Mercury Reducing Biofilmsupporting

confidence: 90%

The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains

Essa¹

2012

Afr. J. Microbiol. Res.

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“…PCR was carried out using the primer set of F1 merA-5 0 TCGTGATGTTCGACCGCT3 0 and F2 merA-5 0 TACTCCCGCCGTTTCCAAT3 0 (Sotero-Martins et al, 2008). The amplification reactions were performed in a total volume of 20 μL by using a thermal cycler (BioRad).…”

Section: Amplification Of Mera Genementioning

confidence: 99%

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Dash

Das

2014

Microbial Biodegradation and Bioremediation

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“…Posteriormente, a placa foi incubada a 37ºC, durante 24 horas. Três a cinco colônias crescidas por placa foram isoladas e armazenadas em meio Luria Bertani (LB) com glicerol a 40% (Sotero-Martins et al 2008).…”

Section: Methodsunclassified

“…Os iniciadores para identificar a sequência do gene merA foram descritos por Sotero-Martins et al (2008), sendo esperado amplicon de 431 pares de bases (pb) para os isolados com mecanismo de resistência ao mercúrio (Hg) via operon merR, sendo eles: Forward primer (F1 merA) -5' TCGTGATGTTCGACCGCT 3' e Reverse primer (F2merA) -5' TACTCCCGCCGTTTCCAAT 3'. Para extração de DNA genômico, utilizou-se a metodologia de lise celular por choque térmico (Nogueira et al 2004).…”

Section: Methodsunclassified

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Bioprospecção de bactérias com potencial tecnológico para biorremediação de ecossistemas impactados por mercúrio e cromo

2014

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A bioprospecção de micro-organismos que possam ser utilizados na biorremediação de ecossistemas degradados é o primeiro passo nas pesquisas que visam utilizar a atividade microbiana para converter substâncias tóxicas em compostos menos nocivos. A Baía de Guanabara, no Sudeste do Brasil, constitui um bioma com grande quantidade e diversidade de poluentes lançados em suas águas, o que compromete a saúde ambiental de tal ecossistema. O presente estudo teve como objetivo avaliar o potencial de biorremediação de bactérias isoladas das praias da Baía de Guanabara. Os micro-organismos foram isolados, identificados, guardados em coleção de cultura e investigados em pesquisas biotecnológicas de biorremediação, quanto à resistência aos metais mercúrio e cromo. Foi avaliada a presença de genes envolvidos nos processos de biorremediação, sendo eles os genes merA, ChrA e ChrB. Dos 169 micro-organismos testados, 8,8% apresentaram resistência ao mercúrio de até 3 mg/L e, em dois isolados, detectou-se a presença do gene merA. Um total de 25,2% dos micro-organismos apresentou resistência ao cromo de até 1 μg/L. Contudo, em nenhum isolado houve presença dos genes ChrA e ChrB nas condições utilizadas.

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A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance

Cited by 30 publications

References 24 publications

The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains

The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Bioprospecção de bactérias com potencial tecnológico para biorremediação de ecossistemas impactados por mercúrio e cromo

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