Lectin-binding properties of Aeromonas caviae strains

Abstract

The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus (EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding lectin) in AeC398 and AeV11 strains, and for Canavalia ensiformes (Con A, D-Man-binding lectin) in ATCC 15468, AeC398, AeQ32 and AeV11 strains, after bacterial growing at 37°C. On the other hand, specific receptors for WGA and EEL were completely abrogated growing the bacteria at 22°C. Binding studies with 125I- labeled lectins from WGA, EEL and Con A were performed. These assays essentially confirmed the selectivity, demonstrated in the agglutination assays of these lectins for the A. caviae strains.

Characteristics of Disease Spectrum in relation to Species, Serogroups, and Adhesion Ability of Motile Aeromonads in Fish

“…Carbohydrate-rich substrates have also been suggested as receptors for Aeromonas adhesins [34]. Therefore, various models such as trout and carp skin and blood, carp kidney, and trout liver were used for adhesion tests in this study.…”

“…Bacterial adhesion processes involve also specific receptor structures on fish cells such as glycoproteins of intestinal gut mucus [ 28 ] or mucin, lactoferrin, and collagen [ 17 ]. Carbohydrate-rich substrates have also been suggested as receptors for Aeromonas adhesins [ 34 ]. Therefore, various models such as trout and carp skin and blood, carp kidney, and trout liver were used for adhesion tests in this study.…”