Molecular analysis of the iap gene of Listeria monocytogenes isolated from cheeses in Rio Grande do Sul, Brazil

Mello, Jozi Fagundes de; Einsfeldt, Karen; Frazzon, Ana Paula Guedes; Costa, Marisa da; Frazzon, Jeverson

doi:10.1590/s1517-83822008000100033

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…This result shows the low degree of polymorphism in the inlA and inlB regions, which has already been shown in previous studies 6,16,17 . This has also already been observed in other genic regions associated with virulence of L. monocytogenes, such as the mpl, prfA and iap genes 8,18 .…”

Section: Rapd Analysissupporting

confidence: 69%

Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

Nes

Riboldi

Frazzon

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Section: Rapd Analysissupporting

confidence: 69%

Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

Nes

Riboldi

Frazzon

et al. 2010

Rev. Soc. Bras. Med. Trop.

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“…Although Brazilian beef and poultry are exported worldwide, limited studies exist which assess the ribotype patterns and virulence potential of Brazilian isolates of L. monocytogenes (Pimenta et al 1999;Lemes-Marques et al 2007;Mello et al 2008). Thus, the objective of this study was to employ molecular and phenotypic subtyping schemes and virulent traits analyses tools to characterize the strains and to establish a possible linkage of pathogen transmission from food to humans.…”

Section: Introductionmentioning

confidence: 99%

Characterization ofListeria monocytogenesisolates of food and human origins from Brazil using molecular typing procedures andin vitrocell culture assays

Bueno

Banerjee

Banada

et al. 2010

International Journal of Environmental Health Research

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The spreading of diseases through foods is a worldwide concern. Here, molecular and in vitro cell-culture assays were employed to characterize 63 Brazilian Listeria monocytogenes isolates (food, 47; clinical, 16). Serotype 4b was the most predominant (49%) followed by (1/2)b (30%), (1/2)a (10%), (1/2)c (6%), 3c (3%) and 3b (2%). Ribotyping yielded 17 ribopatterns, which were grouped into four phylogenetic clusters. Cluster A comprised of 39/63 isolates primarily of food origin, and clusters B, C and D contained both food and clinical isolates. Isolates were positive for virulence determinants prfA, hlyA and inlA: clinical isolates were more invasive to Caco-2 cells and expressed high levels of inlA transcripts than the food isolates. Highly invasive isolates also provoked more Ped-2E9 cells to die by apoptosis than the weakly-invasive strains. These data demonstrate a strong genetic relatedness among clinical and food isolates and suggest transmission of a subset of L. monocytogenes strains from food to humans.

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“…The L. monocytogenes 55 (Lm55) strain was isolated from cheese by the National Agricultural Laboratory of Rio Grande do Sul State (LANAGRO/RS) of the Ministry of Agriculture, Livestock and Food Supply (MAPA/Brazil), and serotyped at the Oswaldo Cruz Institute (State of Rio de Janeiro, RJ, Brazil) as serotype 1/2a (de Mello et al 2008;Nes et al 2010).…”

Section: Bacterial Strainmentioning

confidence: 99%

Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

et al. 2017

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Listeria monocytogenes is a pathogenic microorganism in humans and is frequently transmitted by food. Methods to control the presence of Listeria in foods are necessary. In the present study, transcriptomics of L. monocytogenes grown in the presence of essential oil extracted from Baccharis psiadioides were studied by RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) experiments. The results obtained indicate that essential oil of B. psiadioides has potential bacteriostatic activity at the concentration tested, affecting Listeria cells functioning and development. Responses of the microorganism included upregulation of stress genes and downregulation of virulence genes, such as actA, hly and prfA, indicating a decrease in virulence and in the capacity of the microorganism to cause infection. Thus, the results presented here allow us to conclude that B. psiadioides essential oil may be an alternative means of controlling microorganisms proliferating in foods.

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Molecular analysis of the iap gene of Listeria monocytogenes isolated from cheeses in Rio Grande do Sul, Brazil

Cited by 8 publications

References 14 publications

Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

Characterization ofListeria monocytogenesisolates of food and human origins from Brazil using molecular typing procedures andin vitrocell culture assays

Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

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