Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Abstract

The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first days of growth. Laccase was the main activity detected under the different conditions and was produced throughout the culture period of the fungus, irrespective of the growth phase. Supplementation of the medium with vegetable oil emulsified with a surfactant induced manganese-dependent peroxidase activity in T. villosa. Higher specific yields of laccase activity were obtained with the addition of copper.

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

“…An increase or decrease in pH can affect fungal growth and enzyme formation. Yamanaka et al (2008) reported the production of ligninase by C. hirsutus and T. villosa CCB176 at pH 4.0 and 5.0, respectively, and our results also showed maximum activity at pH 5.0 for selected mutant strains.…”

Biosurfactants are useful tools for the bioremediation of contaminated soil: a review

“…Yamanaka et al (2008) found that supplementation of a Trametes villosa culture medium with a vegetable oil-surfactant emulsion induced MnP activity and higher Lac activity when copper was added. On the other hand, Zhou et al (2007) showed that white-rot fungi degraded decabromodiphenyl ether (BDE-2009, a widely used flame retardant) and that TW80 and b-cyclodextrin could both increase the biodegradation.…”

A Novel Exo-Glucanase Explored from a <i>Meyerozyma</i> sp. Fungal Strain

“…The results indicated that various types of enzymes (e.g., isozymes) with diverse characteristics might produce from the strain #58 when it was cultured under different pH and temperature conditions. A previous study had suggested that productions of lignolytic enzymes from a basidiomycetous fungal strain were dissimilar under different culture conditions [23]; and, another report had indicated that Aspergillus fumigates Z5 produced different proteins after cultivation with various carbon sources (i.e., glucose, avicel, and rice straw) [24]. It was surmised some cellulolytic enzymes might not be produced or might just be produced at very low level under inappropriate culturing conditions.…”