Catalase activity of different Candida species after exposition to specific antiserum

Abstract

Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases activities were abolished. However, the antisera from C. parapsilosis or C. tropicalis immunized rabbits did not affect the catalase activity of C. albicans; the enzyme of C. albicans was inactivated only by the antiserum to the catalase of own C. albicans. The antiserum to the catalase of C. tropicalis was species-specific and did not cross-react with catalases of C. albicans and C. parapsilosis. The activities of Aspergillus niger and bovine catalases were not affected by the antiserum from any Candida immunized rabbits. This report is a preliminary study of specific antisera that react against intracellular catalase of Candida sp. and neutralize the enzymatic activity. Further study is necessary to develop species-specific antibody once differences in the susceptibility of the Candida species to commonly used antifungal drugs make identification to the species level important.

Fungal Strategies to Evade the Host Immune Recognition

“…Similarly to Aspergillus , Candida spp. possess catalases, superoxide dismutases, and glutathione peroxidases that protect the fungus against respiratory burst [ 131 , 132 , 133 ]. This powerful antioxidant defense system has also been described for a wide range of fungi, including H. capsulatum and P. brasiliensis .…”

Antimicrobial effect of para-alkoxyphenylcarbamic acid esters containing substituted N-phenylpiperazine moiety

“…The incidence of infections caused by Candida species has been rising for decades ( Miyasaka et al , 2008 ). In a survey of positive blood cultures performed in the USA, the Candida species ranked fifth in terms of overall incidence and represented the fourth most common group of nosocomial pathogens isolated from the intensive care units ( Gudlaugsson et al , 2003 ).…”

“…However, in hosts predisposed to candidiasis, for example very low birth weight, immunosupressive, antibiotic, and cytotoxic therapies, diabetes mellitus , organ transplant, tumors, immunocompromised patients affected by AIDS and others, these yeasts may act as pathogens. Commensal Candida species normally inhabit in the oral, nasal and aural cavity, vaginal canal, rectum, and gastrointestinal tract of host may begin the infectious process ( Diaz-Guerra et al , 1997 ; Hellstein et al , 1993 ; Miyasaka et al , 2008 ; Oliveira et al , 2011 ; Rodrigues et al , 2004 ).…”

Fenótipos e perfis de sensibilidade aos antifúngicos de leveduras isoladas da mucosa oral de cães da cidade de Campinas, São Paulo

“…Ao longo das décadas, vem aumentando os relatos de manifestações clínicas em humanos relacionadas com infecções por leveduras (Koneman et al, 2001;Miyasaka et al, 2008;Brito et al, 2009). Como decorrência deste aumento, levando em consideração a extrema proximidade dos cães com os humanos, o conhecimento das leveduraspresentes na cavidade oral desses animais se torna de grande importância, assim como o conhecimento do perfil de sensibilidade frente aos antifúngicos das mesmas Carvalho, 2004;Santin, 2009).…”

“…A candidíase representa um problema importante nos hospedeiros imunocomprometidos, nos quais as manifestações clínicas, se não diagnosticadas e tratadas precocemente, podem variar desde infecções superficiais até infecções sistêmicas, podendo ser fatais (Koneman et al, 2001;Lacaz et al, 2002;Miyasaka et al, 2008).…”