Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) strains in ground beef in São Paulo, Brazil

Bergamini, Alzira Maria Morato; Simões, Marise; Irino, Kinue; Gomes, Tânia A. T.; Guth, Beatriz E. C.

doi:10.1590/s1517-83822007000300032

Cited by 27 publications

(15 citation statements)

References 25 publications

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“…When analyzed by m-PCR, 46 STEC isolates (15.9%) harbored the stx2 gene, 24 isolates carried (8.3%) stx1 and 15 isolates (5.2%) had both genes. This result differed from a previous report (Bergamini et al, 2007) that showed the dominance of the stx1 gene in cattle. However, other studies have shown agreement with our study (Zschöck et al, 2000).…”

Section: Discussioncontrasting

confidence: 99%

Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms

et al. 2014

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To estimate the prevalence of Escherichia coli with potential pathogenicity in cattle farm in South Korea, a total of 290 E. coli isolates were isolated from cattle farms over a period of 2 years in South Korea. These were examined for phenotypic and genotypic characteristics including antimicrobial susceptibility, serotype, and gene profiles of virulence and antimicrobial resistance. The most dominant virulence gene was f17 (26.2%), followed by stx2 (15.9%), ehxA (11.0%), stx1 (8.3%), eae (5.2%), and sta (4.1%). Some shiga-toxin producing E. coli isolates possessed eae (15.9%). All isolates except for one showed resistance to one or more antimicrobials, with 152 isolates exhibiting multidrug-resistance. The most prevalent resistance phenotype detected was streptomycin (63.1%), followed by tetracycline (54.5%), neomycin (40.3%), cephalothin (32.8%), amoxicillin (30.0%), ampicillin (29.7%), and sulphamethoxazole/trimethoprim (16.6%). The associated resistance determinants detected were strA-strB (39.0%), tet(E) (80.0%), tet(A) (27.6%), aac(3)-IV (33.1%), aphA1 (21.4%), bla TEM (23.8%), and sul2 (22.1%). When investigated by O serotyping and PFGE molecular subtyping, the high degree of diversity was exhibited in E. coli isolates. These results suggest that E. coli isolates from South Korean cattle farms are significantly diverse in terms of virulence and antimicrobial resistance. In conclusion, the gastroinstestinal flora of cattle could be a significant reservoir of diverse virulence and antimicrobial resistance determinants, which is potentially hazardous to public health.

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Section: Discussioncontrasting

confidence: 99%

Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms

et al. 2014

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“…In Brazil, meat and meat products are the most common foods investigated for the presence of STEC. The percentage of meat and meat products contaminated by STEC ranged from 0 to 20·4% (Cerqueira et al ; Rigobelo et al ; Bergamini et al ; Rodolpho and Marin, ; Rigobelo et al ; Lucatelli ; Loiko et al ; Peresi et al ; Ristori et al ; Castro et al ). However, we highlight the study by Santos et al (), which observed that 80% of the fresh beef collected in the industry processing line was contaminated with STEC (Table ).…”

Section: Occurrence Of Stec In Foodmentioning

confidence: 99%

Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil

Gonzalez

Cerqueira

2019

J Appl Microbiol

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Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC contaminating the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7 remains the most frequent serotype associated with disease. In Brazil, the prevalence of STEC reaches values as high as 90% in cattle and 20% in meat products which may impact the Brazilian food export trade. However, only few reports are related to human disease. The stx1 gene prevails in cattle, whereas the stx2 gene is more frequent in food. Several STEC serotypes have been isolated from cattle and food in Brazil, including the O157:H7, O111:NT, NT:H19 as well as O26 and O103 serogroups. O113: H21 STEC strains are frequent in ruminants and foods but with no report in human disease. The virulence profile of Brazilian STEC strains from cattle and food suggests a pathogenic potential to humans, although some differences with clinical strains have been detected. Further studies, employing recent and more discriminative techniques are in need to better clarify their virulence potential.

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“…Another country of South American and one of the largest beef producers in the world, Brazil has a STEC prevalence of 3.5% in meat products sold in retail markets, with all strains being positive for the ehxA gene responsible for the coding of enterohemolysin and Stx2 toxin, the main gene involved in the most severe cases of the disease (Bergamini and others ).…”

Section: Stec Occurrence and Foodborne Outbreaksmentioning

confidence: 99%

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

Castro

Carvalho

Conté-Júnior

et al. 2017

Comp Rev Food Sci Food Safe

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Historically, Escherichia coli is among the most studied organisms and serves as the basis for understanding many fundamental biochemical and genetic concepts. In addition, it displays 9 pathogenesis groups, with the Shiga toxin-producing (STEC) group being the main representative regarding foodborne pathogenesis. Its typical characteristic is the presence of 2 distinct toxins and variants: stx1 (stx1a, stx1c, and stx1d), and stx2 (stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, and stx2g). The main challenge regarding the study of E. coli is the standardization of a high sensitivity method including all pathotypes, that allows for enrichment of STEC cells and a decrease of background microbiota. The ability of some E. coli cells belonging to other pathogenic groups, such as O104:H4, to acquire genes unique to the STEC group, increases the pathogenic power and the risk of new outbreaks related to these bacteria. In addition, animals with a high concentration of pathogenic E. coli cells present in feces (above 10 4 CFU/g), designated as supershedding animals, may be the primary transmission factor among ruminants. Therefore, the purpose of this review is to address pathogenicity factors and the importance of supershedding animals in the transmission of this pathogen, discussing the main methods currently applied, to focus on the occurrence of STEC in beef.

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Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) strains in ground beef in São Paulo, Brazil

Cited by 27 publications

References 25 publications

Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms

Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms

Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

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