Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia

Ristanović, Elizabeta; Kitamura, Kayoko; Masuzawa, Toshiyuki; Milutinović, Milica; Čekanac, Radovan; Stajković, N; Zivanovic, Danijela M.

doi:10.1590/s1517-83822007000100004

Cited by 5 publications

(6 citation statements)

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“…B. burgdorferi s.l. was first isolated in 1992 from the spleen of an Apodemus flavicollis mouse in Belgrade and after that from Ixodes ricinus ticks ( Ristanovic et al , 2007 ). Since the basic natural way for Borrelia infection is through the bite of an infected vector, the prevalence of these pathogens in ticks represents an important risk indicator for human populations ( Radulovic et al , 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of <italic>Borrelia burgdorferi</italic> in occupationally exposed persons in the Belgrade area, Serbia

Jovanović¹,

Atanasievska²,

Protic-Djokic³

et al. 2015

Braz. J. Microbiol.

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Lyme disease (LD) is a natural focal zoonotic disease caused by Borrelia burgdorferi, which is mainly transmitted through infected Ixodes ricinus tick bites. The presence and abundance of ticks in various habitats, the infectivity rate, as well as prolonged human exposure to ticks are factors that may affect the infection risk as well as the incidence of LD. In recent years, 20% to 25% of ticks infected with different borrelial species, as well as about 5,300 citizens with LD, have been registered in the Belgrade area. Many of the patients reported tick bites in city’s grassy areas. The aim of this study was to assess the seroprevalence of B. burgdorferi in high-risk groups (forestry workers and soldiers) in the Belgrade area, and to compare the results with healthy blood donors. A two-step algorithm consisting of ELISA and Western blot tests was used in the study. Immunoreactivity profiles were also compared between the groups. The results obtained showed the seroprevalence to be 11.76% in the group of forestry workers, 17.14% in the group of soldiers infected by tick bites and 8.57% in the population of healthy blood donors. The highest IgM reactivity was detected against the OspC protein, while IgG antibodies showed high reactivity against VlsE, p19, p41, OspC, OspA and p17. Further investigations in this field are necessary in humans and animals in order to improve protective and preventive measures against LD.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence of <italic>Borrelia burgdorferi</italic> in occupationally exposed persons in the Belgrade area, Serbia

Jovanović¹,

Atanasievska²,

Protic-Djokic³

et al. 2015

Braz. J. Microbiol.

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“…Previously, Milutinović et al (2006) detected a significant presence of B. burgdorferi sensu lato in I. ricinus ticks from Serbia using dark-field microscopy. Furthermore, three B. burgdorferi sensu lato strains isolated from I. ricinus ticks in the Belgrade area were identified as B. burgdorferi sensu stricto and B. afzelii by molecular biological technique (Ristanović et al 2007). Incidence rates of Lyme borreliosis and Tularemia are 5.6-11.1 and 0.1-0.8/100.000 inhabitants between 2001 and 2006, respectively, in Serbia, while only one HGA patients has been reported so far (Ðokić et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia

et al. 2008

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To evaluate the prevalence rate of tick-borne bacterial pathogens, unfed adult Ixodes ricinus ticks were collected from vegetation in 2001, 2003, and 2004 at 18 localities throughout Serbia. A total of 287 ticks were examined by PCR technique for the presence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Francisella tularensis. The highest prevalence rate was that for B. burgdorferi sensu lato (42.5%), followed by A. phagocytophilum (13.9%) and F. tularensis (3.8%). The presence of five B. burgdorferi sensu lato genospecies, namely, B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, and B. valaisiana was identified by restriction fragment length polymorphism (RFLP) analysis. The most frequent B. burgdorferi sensu lato genospecies was B. lusitaniae, followed by B. burgdorferi sensu stricto. Co-infection by B. burgdorferi sensu stricto and B. lusitaniae was frequently observed. Co-infection by B. burgdorferi sensu lato and A. phagocytophilum and co-infection by B. burgdorferi sensu lato and F. tularensis appeared in 24 ticks. Sequencing of p44/msp2 paralogs of Serbian A. phagocytophilum showed that they were unique and distinct from those of A. phagocytophilum in US and UK. This is the first report of B. garinii, B. lusitaniae, B. valaisiana, as well as A. phagocytophilum and F. tularensis infected ticks in Serbia. These findings indicate a public health threat in Serbia of tick-borne diseases caused by B. burgdorferi sensu lato, A. phagocytophilum and F. tularensis.

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“…Due to the fact that beside B. burgdorferi s.s., abundant presence of B. afzelii was confi rmed in the Belgrade area [12][13][14] we additionally performed WB with a B. afzelii strain (human skin isolate obtained from University of Ljubljana, Slovenia) where positive reaction presented a reactivity with at least two of nine protein bands (p83/100, p58, p43, p39, p30, OspC, p21, p18/p17 and p14) (criteria of Hauser et al) [18]. A statistical analysis revealed that there was a high compliance of the results of these two WB assays (article in press).…”

Section: Discussionmentioning

confidence: 90%

“…Five different Borrelia genospecies were detected in I. ricinus ticks from the Belgrade region: B. burgdorferi s.s., B. garinii, B. afzelii, B. valaisiana, and B. lusitaniae [12,13]. In addition, three strains belonging to pathogenic B. burgdorferi s.s. and B. afzelii genospecies were isolated from small rodents captured in the Belgrade area [14].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence Of IgG Antibodies Against Borrelia burgdorferi In Dogs In Belgrade Area, Serbia

et al. 2015

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In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofl uorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was signifi cantly more sensitive than IFA (84.8 vs 67.9%), whereas the specifi cities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignifi cant. These results are the fi rst report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.

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Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia

Cited by 5 publications

References 10 publications

Seroprevalence of <italic>Borrelia burgdorferi</italic> in occupationally exposed persons in the Belgrade area, Serbia

Seroprevalence of <italic>Borrelia burgdorferi</italic> in occupationally exposed persons in the Belgrade area, Serbia

Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia

Seroprevalence Of IgG Antibodies Against Borrelia burgdorferi In Dogs In Belgrade Area, Serbia

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