Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses

Liberatore, Ana Maria Alvim; Tomita, Sandra Kimie; Vieira, Mônica A. M.; Toti, Cyro; Heckmaier, João; Gomes, Tânia A. T.

doi:10.1590/s1517-83822007000100003

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Presence of specific sized amplicon in the PCR here confirmed the isolates as E. coli. This PCR protocol has also been used by others for the detection of E. coli [23], [24], [25]…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and antibiogram of bacterial flora from rectum of horses

Begum¹,

Sarker²,

Khanam³

et al. 2022

GSC Biol. Pharm. Sci.

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In the present study, we isolated, identified and characterized bacteria in rectal swabs from apparently healthy horses around the campus of the Bangladesh Agricultural University (BAU). The study also focused on the determining the prevalence and investigation of drug sensitivity and resistant pattern of the isolates. For this purpose, 20 rectal swab samples were collected from Veterinary Teaching Hospital, BAU; D. C. park (Joinul Abedin Park), Mymensingh and Torar mor, Mymensingh. Most of these samples were found positive for E. coli, Staphylococcus spp. and Salmonella spp. Based on the origin of the samples, the highest prevalence of E. coli, Staphylococcus aureus and Salmonella spp. was found in BAU campus and Torar mor (100%), BAU campus and Torar mor (100%) and Joinul Abedin Park (80%) respectively. The overall prevalence of E. coli, S. Aureus and Salmonella spp. was 93%, 86.67% and 24.44% respectively. For the confirmation of identification and characterization of these bacteria staining, cultural, biochemical studies and Polymerase Chain Reaction (PCR) were accomplished. The antibiogram profiles exhibited that E. coli, S. Aureus and Salmonella spp. were highly sensitive to Erythromycin, Gentamycin and Ciprofloxacin respectively

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“…Presence of specific sized amplicon in the PCR here confirmed the isolates as E. coli. This PCR protocol has also been used by others for the detection of E. coli [23], [24], [25]…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and antibiogram of bacterial flora from rectum of horses

Begum¹,

Sarker²,

Khanam³

et al. 2022

GSC Biol. Pharm. Sci.

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“…For example, enteroaggregative and diffusely adhering E . coli may be defined by their degree of adherence to HeLa cells and their cytotoxic activity in vitro [ 7 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance, pathotypes, and pathogen-host interactions in Escherichia coli from hospital wastewater in Bulawayo, Zimbabwe

Mbanga

Kodzai²,

Oosthuysen³

2023

PLoS ONE

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This study aimed to characterise E. coli strains isolated from hospital wastewater effluent in Bulawayo, Zimbabwe, using both molecular and cytological approaches. Wastewater samples were aseptically collected from the sewerage mains of a major public referral hospital in Bulawayo province weekly for one month. A total of 94 isolates were isolated and confirmed as E. coli through biotyping and PCR targeting the uidA housekeeping gene. A total of 7 genes (eagg, eaeA, stx, flicH7, ipaH, lt, and st genes) coding for virulence in diarrheagenic E. coli were targeted. Antibiotic susceptibility of E. coli was determined against a panel of 12 antibiotics through the disk diffusion assay. The infectivity status of the observed pathotypes was investigated using HeLa cells through adherence, invasion, and intracellular assay. None of the 94 isolates tested positive for the ipaH and flicH7genes. However, 48 (53.3%) isolates were enterotoxigenic E. coli (ETEC) (lt gene positive), 2 (2.13%) isolates were enteroaggregative E. coli (EAEC) (eagg gene), and 1 (1.06%) isolate was enterohaemorrhagic E. coli (EHEC) (stx and eaeA). A high level of sensitivity was observed in E. coli against ertapenem (98.9%), and Azithromycin (75.5%). The highest resistance was against ampicillin (92.6%) and sulphamethoxazole—trimethoprim (90.4%). Seventy-nine (84%) E. coli isolates exhibited multidrug resistance. The infectivity study results indicated that environmentally isolated pathotypes were as infective as the clinically isolated pathotypes for all three parameters. No adherent cells were observed using ETEC, and no cells were observed in the intracellular survival assay using EAEC. This study revealed that hospital wastewater is a hotspot for pathogenic E. coli and that the environmentally isolated pathotypes maintained their ability to colonise and infect mammalian cells.

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Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses

Cited by 2 publications

References 28 publications

Isolation, identification and antibiogram of bacterial flora from rectum of horses

Isolation, identification and antibiogram of bacterial flora from rectum of horses

Antibiotic resistance, pathotypes, and pathogen-host interactions in Escherichia coli from hospital wastewater in Bulawayo, Zimbabwe

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