Performance of a latex agglutination test in the diagnosis of acute gastroenteritis by rotavirus

Abstract

The aim of this work was to determine the performance of latex agglutination test (LAT) for evaluating children acute gastroenteritis by rotavirus. The LAT showed good sensitivity, as well as specificity and predictive positive value and due to its simplicity and speed, it has been suitable for rotavirus diagnosis in hospital laboratories.

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

“…21,34,43,44 Agreement beyond chance the tests between LA and PAGE showed regular/good agreement, demonstrating low sensitivity for latex agglutination test, as shown in other studies. 20,[45][46][47] Regarding specificity, some authors report that, unlike our findings, they have found values above 90%, where the specificity obtained in this study was 81.9%. 42,48 In general, the results obtained by PAGE and EIA had strong agreement, unlike the findings for PAGE and LA.…”

“…Molecular techniques such as polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR) are used to determine the RNA migration patterns and virus genotyping, respectively. [18][19][20][21] In 2006, the RV vaccine was introduced by the Brazilian National Immunization Program for children younger than six months. Since then, the impact of this immunization has been analyzed by monitoring the decrease of children hospitalization due to gastroenteritis and by laboratorial surveillance based on viral antigen tests results.…”

The Histopathological Effect of Rotavirus in Small Intestine of Mice Isolated from Different Area of Iraq

“…The detection of the viral agent can be performed by electronic microscopy, polyacrylamide gel electrophoresis (PAGE), immunofluorescence, radioimmune assay, reverse passive hemagglutination, enzyme immunoassays (EIA), chromatographic immunoassay, and reverse transcriptase with polymerase chain reaction. Among these assays, chromatographic immunoassay was reported as a being easy to perform in a short time, for diagnosis and control of the disease caused by rotavirus in humans [4]. The infection percentage of rotavirus in Iraq in the period between 2012 to 2017 were, at 2012 the positive cases 550 from 977 (56%), 2013 were 482 positive from 1159 (41%), 2014 were 271 from 726 (37%), 2015 were 464 from 638 (72.7%), 2016 were 497 from 569 (87%) and 2017 128 positive cases from 222 (57.6%), According to statistic from public health directorate done at Central Public Health Laboratory (CPHL) with Control Disease Center in Iraq Baghdad from 11 centers sites in Iraq.…”

Comparative evaluation of antigen ELISA technique and PCR for detection of Rotavirus in stool samples of pediatric patients with diarrhea

“…Several techniques have been developed to detect rotavirus in stool samples, including electron microscopy, polyacrylamide gel electrophoresis (PAGE) of viral nucleic acid, various immuno assays, and PCR-based molecular methods (1) . (8) . The performance of those rapid tests indicated that they could be used for screening but not for confirmation of the disease.…”

“…Laboratory diagnosis of rotavirus infection is usually performed by antigen detection, using enzyme immunoassay or LA techniques, which have a sensitivity and specificity above 90%. Molecular techniques such as PAGE and RT-PCR are used to determine the RNA migration patterns and virus genotyping, respectively (10)(11)(12)(13) . ELISA and RT-PCR are being used in research settings.…”