Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis

Chies, Jocelei Maria; Dias, Ana Christina de Oliveira; Maia, Helio; Braga, Luis Paulo da Silva; Astolfi-Filho, Spártaco

doi:10.1590/s1517-83822006000400027

Cited by 2 publications

(1 citation statement)

References 32 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bacterium was identified following Isola, De Vries, and Chu (1994) and Chies, Dias, Maia, Braga, and Astolfi-Filho (2006) methodology. The bacterial genomic DNA was purified using a QIAGEN Genomic Tip 20/G (QIAGENGmbH), yielding 13 μg from 2 mL.…”

Section: Molecular Taxonomy Based In 16s Rrna Gene Sequencementioning

confidence: 99%

Biosurfactants produced by Microbacterium sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

Lima

Pereira

Batista

et al. 2017

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

ABSTRACT. Endophytic bacteria isolated from Eichhornia crassipes (Mart) Solms., collected in oil contaminated wastewater of effluent generated by Petrobras refinery in Manaus were investigated to determine their potential for producing biosurfactants. Assay with 2.6-dichlorophenol indophenol (DCPIP) indicator to verify hydrocarbon biodegradation activity; oil emulsification test; drop-collapse method; surface tension and growth curve of biosurfactant production. The M87 Microbacterium sp. strain chosen for this work was identified by the sequencing of the rDNA region and the . The different techniques of chemical analysis used for the identification of the biosurfactant isolate indicated that this is probably a long -chain fatty acid lipid type, which may be used in the future as both biosurfactant in decontamination processes of hydrocarbon-polluted areas or as bioemulsifier in countless processes, since it exhibited no toxicity as determined by Alamar Blue assay.Keywords: bioremediation, Eichhornia, diesel oil.Biossurfactantes produzidos por Microbacterium sp., isolada de macrófita aquática em área impactada por hidrocarbonetos no rio Negro, Manaus, Amazonas RESUMO. Foram investigadas bactérias endofíticas isoladas de Eichhornia crassipes (Mart.) Solms., coletadas em águas contaminadas com resíduos de petróleo em um afluente da refinaria da Petrobrás/Manaus, para avaliação da produção de biossurfactantes. Para selecionar o micro-organismos à produção e caracterização de biossurfactantes, foram realizados os seguintes testes: a descoloração do indicador 2,6 indofenol (DCPIP), emulsificação do diesel, colapso da gota, tensão superficial e curva de produção. A caracterização química foi realizada por meio das técnicas de FT-IR, UFLC/MS e RMN1H. A bactéria M87 Microbacterium neste estudo, foi identificada pelo sequenciamento da região rDNA e produziu 3g L -1 de biossurfactantes utilizando o diesel como fonte de carbono, mostrando-se eficiente na ação biodegradadora do petróleo, por meio do teste de Indofenol (DCPIP). As frações obtidas, mostraram-se eficazes na redução da tensão superficial da água abaixo de 40 mN m -1 , com destaque para a fração 1 que reduziu a tensão superficial para 34,17 mN m -1 . Pelas análises química utilizadas, pode-se inferir que, provavelmente, se trata de um ácido graxo de cadeia longa, que pode ser utilizado futuramente tanto como biossurfactante em processos de descontaminação de ambientes impactados por hidrocarbonetos, assim como bioemulsificante em inúmeros processos uma vez que não apresentou toxicidade por meio do teste realizado.Palavras-chave: biorremediação, Eichhornia, diesel.

show abstract

Section: Molecular Taxonomy Based In 16s Rrna Gene Sequencementioning

confidence: 99%

Biosurfactants produced by Microbacterium sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

Lima

Pereira

Batista

et al. 2017

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

show abstract

The restriction modification system of Bacillus licheniformis MS1 and generation of a readily transformable deletion mutant

Huff

Muth

Naumer³

et al. 2017

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Restriction modification systems (R-M systems), consisting of a restriction endonuclease and a cognate methyltransferase, constitute an effective means of a cell to protect itself from foreign DNA. Identification, characterization, and deletion of the restriction modification system BliMSI, a putative isoschizomer of ClaI from Caryophanon latum, were performed in the wild isolate Bacillus licheniformis MS1. BliMSI was produced as recombinant protein in Escherichia coli, purified, and in vitro analysis demonstrated identical restriction endonuclease activity as for ClaI. A recombinant E. coli strain, expressing the heterologous bliMSIM gene, was constructed and used as the host for in vivo methylation of plasmids prior to their introduction into B. licheniformis to improve transformation efficiencies. The establishment of suicide plasmids in the latter was rendered possible. The subsequent deletion of the restriction endonuclease encoding gene, bliMSIR, caused doubled transformation efficiencies in the respective mutant B. licheniformis MS2 (∆bliMSIR). Along with above in vivo methylation, the establishment of further gene deletions (∆upp, ∆yqfD) was performed. The constructed triple mutant (∆bliMSIR, ∆upp, ∆yqfD) enables rapid genome manipulation, a requirement for genetic engineering of industrially important strains.

show abstract

Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis

Cited by 2 publications

References 32 publications

<b>Biosurfactants produced by <i>Microbacterium</i> sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

<b>Biosurfactants produced by <i>Microbacterium</i> sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

The restriction modification system of Bacillus licheniformis MS1 and generation of a readily transformable deletion mutant

Contact Info

Product

Resources

About