Isolation of Sporothrix schenkii from the nails of healthy cats

Souza, Lorena Leonardo de; Nascente, Patrícia da Silva; Nobre, M. O.; Meinerz, Ana Raquel Mano; Meireles, Mário Carlos Araújo

doi:10.1590/s1517-83822006000300031

Cited by 26 publications

(25 citation statements)

References 7 publications

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“…The authors attributed the low percentage of isolation to the fact that São Paulo is not an endemic area for sporotrichosis. In contrast, a study performed in Rio Grande [27] (RS; South Brazil) in a shelter with 90 animals with or without sporotrichosis, reported fungal isolation from the nails of seven healthy cats (29.1%) amongst all 24 healthy ones as detected by direct claw impressions in a culture medium. This finding may be associated with the presence of Sporothrix conidia in the paws or in soil fragments attached to them and not necessarily to the nail exclusive colonization.…”

Section: Discussionmentioning

confidence: 96%

Domestic feline contribution in the transmission of Sporothrix in Rio de Janeiro State, Brazil: a comparison between infected and non-infected populations

et al. 2018

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BackgroundSporotrichosis is a neglected zoonosis caused by pathogenic fungi belonging to the Sporothrix schenckii complex. In Rio de Janeiro state, this disease reached an epidemic status with over 4700 domestic felines and around 4000 humans affected since the mid-90s. The present study evaluated clinical and epidemiological aspects and also the frequency of colonization and infection by these fungi in healthy cats and among those with suspicious cutaneous lesions, inhabiting four Rio de Janeiro state distinct areas.ResultsThree hundred and seventy-one cats were included in two groups: 175 healthy cats [CRG] and 196 cats showing lesions suggesting sporotrichosis [SSG]. Mycological diagnosis allowed SSG animals to be divided in positive [104 cats; +SG] and negative [92 cats; -SG] groups. Nails, oral mucosa and lesions swabs were submitted to culture and potential colonies were subculture for micromorphologycal analysis, dimorphism and molecular tests. In the CRG, only one cat was colonized in the oral cavity [0.57%]; in the -SG group, four animals showed colonization of the nail and/or oral cavity [4.3%]; while the highest frequency of colonization [39.4%] was observed in the +SG. All molecularly typed isolates were identified as S. brasiliensis.ConclusionThe results obtained here indicate that healthy cats have a minor role in sporotrichosis transmission within the state of Rio de Janeiro. Conversely, a higher participation of diseased feline in sporotrichosis transmission was evidenced, especially by the colonization of their oral cavity. Sporothrix brasiliensis equally affects and colonizes animals from distinct Rio de Janeiro state areas. Thus, we hypothesize that sporotrichosis is a uniform endemic throughout the state, whose transmission depends mainly on the contact with cats with sporotrichosis. Since Rio de Janeiro displays a world unique epidemic model of the disease, not fully understood, data on the infected and non-infected animals can be of major importance for future strategies of sporotrichosis prevention and control. Finally, considering the importance of the current concept of “one health”, the experience here observed can be helpful for distinct epizootias and/or zoonosis.

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Section: Discussionmentioning

confidence: 96%

Domestic feline contribution in the transmission of Sporothrix in Rio de Janeiro State, Brazil: a comparison between infected and non-infected populations

et al. 2018

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“…Baixa carga fúngica tecidual em caso com cultura positiva para Sporothrix já foi observada, conforme revisado por Gross et al (2009). Isso demonstra que nem sempre os gatos apresentam um alto potencial zoonótico como geralmente afirmado (Souza et al 2006, Antunes et al 2009, Gross et al 2009, Schubach et al 2012) e podem ter lesões que se assemelham a esporotricose canina (Gross et al 2009), dificultando sobremaneira o diagnóstico.…”

Section: Discussionunclassified

“…A esporotricose é considerada um risco ocupacional para indivíduos que trabalham com solo, plantas ou materiais vegetais, pois a principal forma de infecção é através da inoculação do fungo devido à perfuração por espinhos ou lascas de madeira (Ginn et al 2007, Schubach et al 2012. Transmissão zoonótica, através de mordidas ou arranhões de ratos, tatus, gatos e cães, também ocorre (Schubach et al 2012), sendo os gatos a espécie animal mais associada com essa forma de contágio, devido principalmente à grande quantidade de leveduras nas lesões, mas também por carrearem o agente nas unhas e na cavidade oral (Souza et al 2006, Antunes et al 2009, Schubach et al 2012.…”

Section: Introductionunclassified

Características clínico-epidemiológicas, histomorfológicas e histoquímicas da esporotricose felina

et al. 2016

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RESUMO.-Esporotricose é uma infecção fúngica causada por espécies do complexo Sporothrix, vista com maior frequência em gatos, equinos e cães. Os principais objetivos deste estudo retrospectivo foram caracterizar os aspectos histomorfológicos e histoquímicos da esporotricose em 10 gatos, além de avaliar os aspectos epidemiológicos, clíni-cos e os achados macroscópicos desses 10 casos, obtidos dos protocolos de biópsias e necropsias dos arquivos do Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria. A doença acometeu predominantemente gatos machos, sem raça definida e apresentou-se principalmente na forma cutânea disseminada. As lesões macroscópicas caracterizaram-se como nódulos cutâneos (ulcerados ou não) e como massas e placas ulceradas. Na histopatologia observou-se uma relação entre a quantidade de leveduras observada e dois padrões de resposta infla- glaukommers@yahoo.com Sporotrichosis is a mycotic infection caused by the Sporothrix complex species, affecting mostly cats, horses, and dogs. The main objectives of this retrospective study were to characterize the histomorphological and histochemical aspects of sporotrichosis in 10 cats, and also to evaluate epidemiological and clinical aspects, and gross findings obtained from the biopsy and necropsy protocols of these ten cases, obtained from the archives of the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. The disease affected mostly male mongrel cats, and was mostly observed in the cutaneous disseminated form. Gross lesions were characterized by cutaneous nodules (ulcerated or not) and by ulcerated masses or plaques. By histopathology, there were a relationship between the fungal load and two patterns of inflammatory response. The first pattern was characterized by high fungal load, and most yeasts were inside of numerous macrophages with abundant, many times vacuolated cytoplasm. The amount of neutrophils ranged from mild to moderate in this pattern. The second pattern was characterized by numerous epiteliod cells and many neutrophils. The fungal load was low and most yeasts were observed in the extracellular space. The yeasts were round, oval or cigar-shaped. Several histochemical techniques, such as Grocott's silver stain, periodic Schiff acid, and Alcian blue were utilized and they made it easier to visualize, to characterize the morphology, and to quantify the organisms. The Giemsa stain allowed visualizing the agent, but it not allowed to sharply highlight them from other intralesional components. Organisms were negative for melanin granules through the Fontana-Masson stain in all cases. The histomorphological and histochemical study allowed demonstrating determinant fungal characteristics to the establishment of the diagnosis by means of this diagnostic tool.INDEX TERMS: Feline sporotrichosis, Sporothrix spp. complex, diseases of cats, subcutaneous mycosis.

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“…The southeast region of Brazil, especially the state of the Rio de Janeiro, experienced an epidemic of sporotrichosis, with 100s of cases described in animals and humans as well. In the state of Rio Grande do Sul the disease has been reported occasionally, however, little is known about the epidemiology and therapeutics of feline sporotrichosis (Barros et al., 2004; Schubach et al., 2004a;Xavier et al., 2004; Souza et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

Feline Sporotrichosis in the Southern Region of Rio Grande Do Sul, Brazil: Clinical, Zoonotic and Therapeutic Aspects

Madrid

Mattei

Martins

et al. 2010

Zoonoses and Public Health

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Clinical cases of feline sporotrichosis, originating in the Pelotas region and diagnosed at the Laboratory of Infectious Diseases (UFPel), were studied in the period from 2002 to 2006. The animals were evaluated according to the clinical forms of the mycosis, time of lesion appearance, severity of the clinical diagnosis and evolution of cutaneous lesions throughout the treatment period. Mycological analyses, carried out through direct examination, cultivation of tissue samples and exudates of feline lesions all confirmed the diagnosis of sporotrichosis in the 15 animals under study. The cutaneous dissemination form was observed in 10 animals, of which three showed prostration, anorexia and dehydration. The zoonosis occurred in 20% of case studies, and the pet owners and one attendant at a veterinary clinic were infected, developing the fixed and disseminated cutaneous forms. The treatment of mycosis was carried out with itraconazole, 10 mg kg(-1), once a day, on 12 animals. The cure of the clinical symptoms was observed on 50% of the felines. This study demonstrates a good clinical response of felines with sporotrichosis, when they were treated itraconazole and calls the attention for the incidence of human sporotrichosis on people related to the veterinary activity as well as for pet owners.

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Isolation of Sporothrix schenkii from the nails of healthy cats

Cited by 26 publications

References 7 publications

Domestic feline contribution in the transmission of Sporothrix in Rio de Janeiro State, Brazil: a comparison between infected and non-infected populations

Domestic feline contribution in the transmission of Sporothrix in Rio de Janeiro State, Brazil: a comparison between infected and non-infected populations

Características clínico-epidemiológicas, histomorfológicas e histoquímicas da esporotricose felina

Feline Sporotrichosis in the Southern Region of Rio Grande Do Sul, Brazil: Clinical, Zoonotic and Therapeutic Aspects

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