Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat

Schneid, Andréa dos Santos; Rodrigues, Kelly Lameiro; Chemello, Davi; Tondo, Eduardo César; Ayub, Marco Antônio Zacchia; Aleixo, José Antônio Guimarães

doi:10.1590/s1517-83822006000300027

Cited by 38 publications

(16 citation statements)

References 35 publications

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“…The ELISA is a powerful immunological method and has been more frequently used for the detection of foodborne pathogens, such as E. coli (Wang and others 2007; Galikowska and others 2011), Listeria monocytogenes (Portanti and others 2011), S. enteric (Schneid and others 2006), and Vibrio parahaemolyticus (Kumar and others 2011). Nevertheless, as far as we know, using ELISA for detection of Alicyclobacillus has never been reported.…”

Section: Discussionmentioning

confidence: 99%

“…ELISA has been extensively used for screening of bacteria including Salmonella enteric , Escherichia coli , and others in food due to its sensitivity, specificity, rapidity, and simplicity (Schneid and others 2006; Wang and others 2007; Wang and others 2011). Nevertheless, as far as we know, using ELISA for detection of Alicyclobacillus in apple juice or other beverages has never been reported.…”

Section: Introductionmentioning

confidence: 99%

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Development of Polyclonal Antibody‐Based Indirect Enzyme‐Linked Immunosorbent Assay for the Detection of Alicyclobacillus Strains in Apple Juice

Wang¹,

Yue²,

Yuan³

et al. 2012

Journal of Food Science

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A sort of specific polyclonal anti-Alicyclobacillus antibody was generated by immunizing New Zealand white rabbits, and a sensitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for Alicyclobacillus detection in apple juice. A set of experimental parameters such as concentration of antigen, dilutions of the antibody and goat anti-rabbit IgG-horseradish peroxidase conjugate, selection of the blocking reagent, incubation time, and temperature was optimized. The cross-reactivity of the antibody was evaluated by ELISA and the result was consistent with Western blot analysis. The detection limit of the ELISA was about 10(5) colony forming units (CFU)/mL in apple juice samples. Samples were detected by ELISA and conventional culture method, and the ELISA results gave a good agreement with the results obtained by plating on Alicyclobacillus acidoterrestris medium agar. ELISA takes a total detection time of 6 to 7 h, which is less than the time of conventional techniques requiring more than 24 to 48 h. These results indicated that the established ELISA was a potential useful analytical method for detection of Alicyclobacillus in apple juice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Polyclonal Antibody‐Based Indirect Enzyme‐Linked Immunosorbent Assay for the Detection of Alicyclobacillus Strains in Apple Juice

Wang¹,

Yue²,

Yuan³

et al. 2012

Journal of Food Science

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“…4 ELISA is rapid and precise, but its application is limited by the availability of pure antigens and subsequent production of speci¯c antibodies. 5 Nucleic acid ampli¯cation-based methods are preferred because they enable the accurate identi¯ca-tion of target nucleic acid sequences with high sensitivity and speci¯city, and nucleic acid ampli¯-cation methods are less time consuming and less vulnerable to cross-contamination. Among these methods, polymerase chain reaction (PCR) is most commonly used.…”

Section: Introductionmentioning

confidence: 99%

Fast infectious diseases diagnostics based on microfluidic biochip system

Huang

Han

Kou³

et al. 2017

J. Innov. Opt. Health Sci.

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Molecular diagnostics is one of the most important tools currently in use for clinical pathogen detection due to its high sensitivity, speci¯city, and low consume of sample and reagent is keyword to low cost molecular diagnostics. In this paper, a sensitive DNA isothermal ampli¯-cation method for fast clinical infectious diseases diagnostics at aM concentrations of DNA was developed using a polycarbonate (PC) micro°uidic chip. A portable confocal optical°uo-rescence detector was speci¯cally developed for the micro°uidic chip that was capable of highly sensitive real-time detection of ampli¯ed products for sequence-speci¯c molecular identi¯cation near the optical di®raction limit with low background. The molecular diagnostics of Listeria monocytogenes with nucleic acid extracted from stool samples was performed at a minimum DNA template concentration of 3.65 aM, and a detection limit of less than¯ve copies of genomic DNA. Contrast to the general polymerase chain reaction (PCR) at eppendorf (EP) tube, the detection time in our developed method was reduced from 1.5 h to 45 min for multi-target parallel detection, the consume of sample and reagent was dropped from 25 L to 1.45 L. This novel micro°uidic chip system and method can be used to develop a micro total analysis system as a clinically relevant pathogen molecular diagnostics method via the ampli¯cation of targets, with potential applications in biotechnology, medicine, and clinical molecular diagnostics.

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“…However, Druggan-Forsythe-Iversen medium has been recently used to detect C. muytjensii (Bar-Oz et al, 2001; Iverson and Forsythe, 2003). Various other methods such as DNA hybridization, polymerase chain reaction, immunomagnetic beads and immunoenzymatic assays have been used to detect C. muytjensii in various food matrices (Schneid et al, 2006). The enzyme-linked immunosorbent assay (ELISA) has been shown to be a sensitive technique for the detection of several infectious agents (Voller and Bidwell, 1976).…”

Section: Introductionmentioning

confidence: 99%

Development of Chicken Immunoglobulin Y for Rapid Detection of Cronobacter muytjensii in Infant Formula Powder

Kim¹,

Shukla̽²,

Ahmed³

et al. 2012

Korean Journal for Food Science of Animal Resources

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The present study was aimed to produce a chicken polyclonal antibody against Cronobacter muytjensii and to develop an immunoassay for its detection. Purification of anti-C. muytjensii IgY from egg yolk was accomplished using various methods such as water dilution and salt precipitation. As a result, sodium dodecyl sulfate-polyacrylamide gel electrophoresis produced two bands around 30 and 66 kDa, corresponding to a light and a heavy chain, respectively. Indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was performed to determine the effectiveness of the chicken IgY against C. muytjensii. The optimum conditions for detecting C. muytjensii by indirect ELISA and checkerboard titration of the antigen revealed an optimum average absorbance at the concentration of 18 µg/mL, having ca. 10 8 coated cells per well. The anti-C. muytjensii IgY antibody had high specificity for C. muytjensii and low cross-reactivity with other tested pathogens. In this assay, no cross-reactivity was observed with the other genera of pathogenic bacteria including Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Bacillus cereus, Enterobacter aerogenes, Salmonella Enteritidis and Listeria monocytogenes. In addition, detection of C. muytjensii in infant formula powder showed a low matrix effect on the detection curve of IC-ELISA for C. muytjensii, with similar detection limit of 10 5 CFU/mL as shown in standard curve. These findings demonstrate that the developed method is able to detect C. muytjensii in infant formula powder. Due to the stable antibody supply without sacrificing animals, this IgY can have wide applications for the rapid and accurate detection of C. muytjensii in dairy foods samples.

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Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat

Cited by 38 publications

References 35 publications

Development of Polyclonal Antibody‐Based Indirect Enzyme‐Linked Immunosorbent Assay for the Detection of Alicyclobacillus Strains in Apple Juice

Development of Polyclonal Antibody‐Based Indirect Enzyme‐Linked Immunosorbent Assay for the Detection of Alicyclobacillus Strains in Apple Juice

Fast infectious diseases diagnostics based on microfluidic biochip system

Development of Chicken Immunoglobulin Y for Rapid Detection of Cronobacter muytjensii in Infant Formula Powder

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