Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent

Abstract

Enzymes, and particularly proteases, have become an important and indispensable part of industrial processes such as laundry detergents, pharmaceuticals and food products. Detergents such as Tide ® , Ariel ® and Biz ® contain proteolytic enzymes, most of them produced by members of the genus Bacillus. This paper describes the compatibility of protease produced by the thermophilic Bacillus sp, with commercial laundry detergent. Stability studies indicated that this enzyme retained about 95% and 74% of its maximum activity after 1h at 60ºC in the presence of glycine in combination with MnSO4 and CaCl2, respectively. No inhibitory effect was observed at 1.0-5.0 mM of EDTA. Triton X-100 inhibited the enzyme in all the concentrations tested. The enzyme was unstable in a 5% (v/v) concentration of peroxide solution. The protease retained more than 80% and 65% of its activity after 30 min incubation at 60ºC in the presence of Tide ® and Cheer ® detergents, respectively. After supplementation of CaCl2 (10 mM) and glycine (1 mM), the enzyme in Tide ® detergent retained more than 85% of its activity after 1h. Based on these findings, Bacillus sp. protease shows a good potential for application in laundry detergents.

Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

“…This result is positively correlated with the Triton X-100-and SDS-stable protease production reported in Bacillus clausii I 52 (Joo et al 2003) and Bacillus sp. (Nascimento and Martins 2006). Our results are also consistent with reports of polyethylene glycol-induced lipase production by Yarrowia lipolytica (Alberts et al 2003) and surfactant-aided solid-state production of amylase (Goes and Sheppard 1999).…”

Hide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate <i>Bacillus cohnii</i>U3

“…The results of stability study correspond to earlier reports (Nejad et al, 2010;Nascimento and Martins, 2006). The enzyme exhibited good solvent tolerance to all the tested organic solvent.…”

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

“…Throughout half a century, numerous industrially important species belonging to the genus of Bacillus have been discovered (Nascimento & Martins, 2006;Sun et al, 2015;Suwanmanon & Hsieh, 2014) and found to be particularly valuable for genetic and biochemical studies. Several hundred wild-type forms of Bacillus sp.…”