Evaluation of the Diramic system for urine cultures

Abstract

The use of the Diramic system in microbiological diagnosis of urinary tract infections (UTI) was evaluated. This system was developed at the National Center for Scientific Research of Cuba, and it reads turbidimetric changes of microbial growth in culture media at 37ºC incubated for 4 hours. A total of 396 urine specimens were tested in the Laboratory of Microbiology of the School of Medicine -UNESP, Brazil using the Diramic system and the counting of colony forming units per urine millimeter (calibrated loop) as the reference method. The coincidence rate between the two methods was 96.46% (382 urine samples), and the differences in results were not significant (p≤0.05). Sensitivity and specificity rates were 84.37% and 98.80%, respectively. False negative and false positive rates were 2.50% and 1.01%, respectively. The microorganisms isolated from positive urines were: Escherichia coli (68.75%); Klebsiella pneumoniae (10.94%); yeast (6.25%); Pseudomonas aeruginosa (4.69%); Enterobacter cloacae (3.12%); Proteus mirabilis, coagulase negative Staphylococcus, Morganella morganii, and Citrobacter freundii (1.56% each). The Diramic system was effective as screening method for urine cultures, however restrictions in the UTI diagnosis caused by yeasts and patients undergoing antibiotic therapy were negative characteristics of the system.

CHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIBACTERIAL, ANTIFUNGAL, ANTIMYCOBACTERIAL, AND CYTOTOXIC ACTIVITIES OF Talinum paniculatum

“…Featuring similar clinical and epidemiological importance than S. aureus and C. albicans , the bacteria E. coli, S. marcescens, P. aeruginosa , and P. mirabilis are usually found in the human microbiome and, under certain predisposing factors, they are responsible for a range of infections. Due to the intense acquisition and dissemination of genes related to multidrug resistance, infectious diseases caused by these microorganisms have also received notable attention 11 , 14 . In the present study, E. coli, S. marcescens, P. aeruginosa , and P. mirabilis were inhibited in vitro by low concentrations of HX and EtOAc (from 31.2 to 500 µg/mL).…”