Yeasts isolated from sand and sea water in beaches of Olinda, Pernambuco state, Brazil

Loureiro, Silvia Tereza Azedo; Cavalcanti, María Auxiliadora de Queiroz; Neves, Rejane Pereira; Passavante, José Zanon de Oliveira

doi:10.1590/s1517-83822005000400005

Cited by 26 publications

(22 citation statements)

References 12 publications

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“…The water samples were used undiluted. Both the sediment suspensions (1 ml) and the water samples (1 ml) were laid over Sabouraud dextrose agar (Difco TM ; Becton-Dickinson, Sparks, MD) enriched with yeast extract and were incubated at 308C (Pinto et al, 1992;Loureiro et al, 2005). Following microorganism growth, separate colonies were resuspended and seeded on Sabouraud dextrose agar gel and incubated at 308C for 24 h.…”

Section: Methodsmentioning

confidence: 99%

Candida famata modulates toll‐like receptor, β‐defensin, and proinflammatory cytokine expression by normal human epithelial cells

Bahri

Saidane-Mosbahi

Rouabhia

2009

Journal Cellular Physiology

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Candida albicans is no longer the only yeast involved in infectious disorders, as others, such as C. famata, commonly associated with foods as well as terrestrial and marine environments, are being recognized as potential emerging pathogens that cause human candidiasis. We investigated the interaction between C. famata and human epithelial cells using monolayer cultures and an engineered human oral mucosa (EHOM). C. famata was able to adhere to gingival epithelial cells but failed to adopt the hyphal form in the presence/absence of proteins. Interestingly, when cultured onto the engineered human oral mucosa (EHOM), C. famata formed a biofilm and invaded the connective tissue. When normal human gingival epithelial cells were put in contact with C. famata, they expressed high levels of Toll-like receptors (TLR)-2, -4, and -6, but not TLR-9 mARN. The upregulation of TLRs was paralleled by an increase of IL-1beta and TNFalpha, but not IFNgamma mARN expression, suggesting the involvement of specific pro-inflammatory cytokines (IL-1beta and TNFalpha) in the defense against infection with C. famata. The active role of epithelial cells in the innate immunity against C. famata infection was enhanced by their capacity to express high levels of human beta-defensin (HBD)-1, -2, and -3. The upregulation of pro-inflammatory cytokines and antimicrobial peptide expression may explain the growth inhibition of C. famata by the gingival epithelial cells. Overall results provide additional evidence of the involvement of C. famata in the activation of innate immunity and the contribution of human epithelial cells in local defenses against such exogenous stimulations as C. famata infections.

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Section: Methodsmentioning

confidence: 99%

Candida famata modulates toll‐like receptor, β‐defensin, and proinflammatory cytokine expression by normal human epithelial cells

Bahri

Saidane-Mosbahi

Rouabhia

2009

Journal Cellular Physiology

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“…Within Antarctic coastal waters, a diversity of fungi species have been recorded (Bridge & Spooner, 2012; Rosa, 2019), with several endemic taxa being found associated with macroalgae (Loque et al., 2010). In the western South Atlantic (WSA), only a few studies have described the fungal diversity in marine ecosystems, with more focus on using fungi as indicators of pollution in the marine environment (Azedo Loureiro, De Queiroz Cavalcanti, Neves, & De Oliveira Passavante, 2005; Hagler & Mendonça‐Hagler, 1981). Molecular techniques can reveal a much higher diversity of fungi, particularly parasitic and pathogenic taxa which are difficult to isolate without host taxa (Richards et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

Diverse groups of fungi are associated with plastics in the surface waters of the Western South Atlantic and the Antarctic Peninsula

et al. 2020

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Marine plastic pollution has a range of negative impacts for biota and the colonization of plastics in the marine environment by microorganisms may have significant ecological impacts. However, data on epiplastic organisms, particularly fungi, is still lacking for many ocean regions. To evaluate plastic associated fungi and their geographic distribution, we characterised plastics sampled from surface waters of the western South Atlantic (WSA) and Antarctic Peninsula (AP), using DNA metabarcoding of three molecular markers (ITS2, 18S rRNA V4 and V9 regions). Numerous taxa from eight fungal phyla and a total of 64 orders were detected, including groups that had not yet been described associated with plastics. There was a varied phylogenetic assemblage of predominantly known saprotrophic taxa within the Ascomycota and Basidiomycota. We found a range of marine cosmopolitan genera present on plastics in both locations, i.e., Aspergillus, Cladosporium, Wallemia and a number of taxa unique to each region, as well as a high variation of taxa such as Chytridiomycota and Aphelidomycota between locations. Within these basal fungal groups we identified a number of phylogenetically novel taxa. This is the first description of fungi from the Plastisphere within the Southern Hemisphere, and highlights the need to further investigate the potential impacts of plastic associated fungi on other organisms and marine ecosystems.

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“…In Brazil, few studies reported the occurrence of fungi (Gomes et al 2008) and yeasts (Faraco & Faraco 1960, Queiroz 1972, Loureiro et al 2005 in these environments.…”

Section: Introductionmentioning

confidence: 99%

Fungos e Leveduras no estuário da Lagoa dos Patos e Praia do Cassino, RS, Brasil

Silveira¹,

Lobato²,

Abreu³

2013

Atlântica

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FUNGI AND YEASTS IN THE PATOS LAGOON ESTUARY AND CASSINO BEACH, RS, BRAZILThis study evaluated the spatial and temporal variability of the fungi and yeasts in the waters of the estuarine region of the Patos Lagoon and the adjacent oceanic Cassino Beach. Water samples was sampled monthly (March 2007 to June 2008) from three sites the richness of fungi was assessed by cultivating the samples in Sabouraud agar plates with Chloramphenicol. Environmental variables like water temperature, salinity, pH and dissolved inorganic nutrients (phosphate ammonium, nitrite and nitrate) were also analyzed. The most common fungal genera found were Aspergillus, Penicillium, and clusters of nonsporulating fungi and yeasts. Fungi distribution was mainly affected negatively by salinity and positively by ammonium and phosphate.KEYWORDS: spatial variability, temporal variability, salinity, nutrients RESUMO Este estudo avaliou a variabilidade espacial e temporal dos fungos e leveduras nas águas da região estuarina da Lagoa dos Patos e praia do Cassino. Amostras de água foram coletadas mensalmente pelo período de um ano em três pontos distribuídos ao longo do estuário da Lagoa dos Patos e região costeira adjacente. A riqueza de fungos foi avaliada através de cultivos das amostras em placas com ágar Sabouraud com Cloranfenicol. Variáveis ambientais como temperatura da água, salinidade, pH e nutrientes inorgânicos dissolvidos (fosfato, amônia, nitrito e nitrato), também foram analisados. Os gêneros de fungos mais encontrados foram Aspergillus, Penicillium, e agrupamentos de fungos não-esporulados e leveduras. A distribuição de fungos foi afetada negativamente pela salinidade e positivamente pelas concentrações de amônia e fosfato.

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Yeasts isolated from sand and sea water in beaches of Olinda, Pernambuco state, Brazil

Cited by 26 publications

References 12 publications

Candida famata modulates toll‐like receptor, β‐defensin, and proinflammatory cytokine expression by normal human epithelial cells

Candida famata modulates toll‐like receptor, β‐defensin, and proinflammatory cytokine expression by normal human epithelial cells

Diverse groups of fungi are associated with plastics in the surface waters of the Western South Atlantic and the Antarctic Peninsula

Fungos e Leveduras no estuário da Lagoa dos Patos e Praia do Cassino, RS, Brasil

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