Partial characterization of ribosomal operons of Lactobacillus delbrueckii UFV H2b20

Abstract

Ribosomal operons are great tools for microbe community characterization and for microorganisms relationship study, particularly in the case of the acid lactic bacteria. The ribosomal operon of the probiotic strain Lactobacillus delbrueckii UFV H2b20 was partially characterized. A genomic library of this strain was constructed and the clones with partial ribosomal operon were sub-cloned using the shot-gun method for subsequent sequencing with the forward primer. The sequence analysis revealed that the 3' end of the rDNA 16S was following by the short spacer region 1 (16S-23S) and that the 3' end of the rDNA 23S was following by the short spacer region 2 (23S-5S), which preceded the rDNA 5S. In the flanking region of the rDNA 5S gene of this operon rrn, a region encoding six tRNAs was detected.

Fingerprinting of Lab Isolated From Silage, Water, Raw Milk, Starter Culture and Minas Artisanal Cheese from the Campo Das Vertentes Region, Brazil

“…Samples of L. plantarum, L. paracasei and L. brevis were detected in water, milk, endogenous starter culture and, specifically L. plantarum, throughout many ripening stages in different dairy farms. This can be explained due to their role as NSLAB, developing in later stages of ripening regarding higher levels of proteolysis and development of flavor, as well as reducing pathogenic or spoiling microorganisms in cheeses [12][13][14][15][16][17][18][19].…”