Metallo-beta-lactamase producing Pseudomonas aeruginosa strains isolated in hospitals in Recife, PE, Brazil

Magalhäes, Vera; Lins, Ana Kelly; Magalhães, Marcelo

doi:10.1590/s1517-83822005000200005

Cited by 30 publications

(25 citation statements)

References 19 publications

(11 reference statements)

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“…The results of the present study showed that 33% of Imipenem non-susceptible isolates were MBL positive, the previous studies from Korea in 2009 showed that 10.8% of Imipenem non-susceptible isolates were MBL positive (Oie et al, 2009), on the other hand, previous studies from Azarbayejan, Iran showed that 56.73% of Imipenem non-susceptible isolates were MBL positive by Imipenem-EDTA combined disk method (Yousefi et al, 2011). In the present study 95.2 and 100% of Imipenem non-susceptible isolates and MBL producing isolates were multi drug resistant isolates, these findings are in accordance with results obtained by Magalhes et al (2005) which reported that MBL positive isolates were resistant to main anti-pseudomonas drugs. In conclusion, the clinical significance of P. aeruginosa isolates possessing Metallo-β-lactamase enzymes are ultimately judged by their ability to confer in vivo resistance to betalactams and even other classes of antipseudomonas antibiotics, ultimately lead to the patient fails antibiotic therapy.…”

Section: Discussionsupporting

confidence: 90%

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Jazani¹,

Zahedi²,

Garebagi³

2012

Afr. J. Microbiol. Res.

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Detection of metallo-β-lactamase producing Pseudomonas aeruginosa is crucial for the optimal treatment of patients; however there are limited studies on metallo-β-lactamase producing P. aeruginosa isolates from West Azarbayejan, Iran. This study was designed to detect the metallo-β-lactamase in P. aeruginosa isolates. One hundred isolates were collected from clinical specimens submitted to hospital diagnostic laboratories in Urmia/Iran from July to September 2010. The susceptibilities of the isolates to different classes of antibiotics were tested using Müller-Hinton agar disk diffusion method. All isolates of P. aeruginosa were subjected to determination of minimum inhibitory concentrations )MICs) against Imipenem. Imipenem non-susceptible isolates were investigated for metallo-β-lactamase production by the combined disk method. The rates of resistances to antibiotics, as were determined, is as follows: kanamycin (91%), Tobramycin (34%), Ciprofloxacin (16%), Colistin (68%), Ticarcillin (46%), Amikacin (16%), Norfloxacin (23%), gentamicin (33%), Ceftazidime (62%), Ceftizoxime (69%), and Cefepime (39%). Seventy nine isolates (79%) were sensitive (MIC ≤ 4 mg/L) and 21 isolate (21%) were resistant to Imipenem (MIC ≥ 8 mg/L). The rates of resistance to different antibiotics were much higher in Imipenem resistant isolates. Detection of metallo-β-lactamase producing isolates among Imipenem non-susceptible isolates of P. aeruginosa revealed that seven isolates (33.3%) were metallo-β-lactamase positive. Metallo-β-lactamase positive isolates showed high resistances to all tested antibiotics. This result suggests that metallo-β-lactamase producing isolates in hospitals may cause serious infections which can lead to failure in patient's antibiotic therapy.

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Section: Discussionsupporting

confidence: 90%

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Jazani¹,

Zahedi²,

Garebagi³

2012

Afr. J. Microbiol. Res.

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“…Magalhães et al carried out a molecular analysis on 48 strains of P. aeruginosa from Recife/Brazil. They demonstrated that 62.5% of the strains produced metallo-β-lactamase (SPM-1) with a broad resistance profile, such that they were only susceptible to polymyxin and aztreonam [28]. The best association of antimicrobial agents for treating infections due to P. aeruginosa is still a matter of controversy.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa infections: factors relating to mortality with emphasis on resistance pattern and antimicrobial treatment

Pinheiro¹,

Lacerda²,

Melo³

et al. 2008

Braz J Infect Dis

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A retrospective case-control study was conducted to investigate the risk factors for death among intensive care unit patients with Pseudomonas aeruginosa infection. Out of 131 patients investigated, 67 (51.1%) died within 30 days of being diagnosed with this infection. The mean duration of hospital stay before this diagnosis was 28.5 ± 26.5 days. No association was found between bacterial resistance and death in this study (multiresistant p= 0.26; panresistant p= 0.42), but the adequacy of the initial treatment was inversely proportional to the degree of resistance. There was a tendency towards greater mortality among patients who received combination therapy (empirical p= 0.09; definitive p= 0.08), despite the greater frequency of appropriate treatment in these patients and the similar degree of severity in the two groups. This finding may be explained by pharmacodynamic parameters that were not studied or by the extensive use of aminoglycosides in the combination therapy, which play a controversial role in combination therapy due to their potential for renal toxicity. The multivariate analysis in our study demonstrated that age [odds ratio (OR) 1.04], septic shock (OR 15.4) and hypoalbuminemia (OR 0.32) were independent risk factors for death.

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“…According to Marra et al (2006) [12] and Magalhães et al (2005) [13], the SPM-1 enzyme, which is highly prevalent in Brazil, could be equally detected by the 2-MPA method and the EDTA-based method. Zavascki et al (2005) [14] reported that 77.1% of their P. aeruginosa isolates, resistant to IPM, could be detected by 2-MPA and 87.5% of the strains carried the bla SPM-1 gene.…”

Section: Resultsmentioning

confidence: 99%

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Wirth

Picoli²,

Cantarelli

et al. 2009

Braz J Infect Dis

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This study determined the prevalence of metallo-β β β β β-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk. Strains positive were further confirmed using molecular techniques for bla VIM , bla IMP and bla SPM-1 . The prevalence of MBLs from samples of inpatients from the hospital located in Porto Alegre was 30.4% and that of inpatients from Vale dos Sinos hospital was only 3.1%. Only MBL type SPM-1 was detected in these samples by molecular analysis and all were detected by the Etest® MBL strips. The prevalence of P. aeruginosa that produce MBLs can be markedly different in distinct geographical areas, even among different hospitals in the same area. In our study, the EDTA-based method was the only method able to detect all strains harboring the SPM-1 enzyme.

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Metallo-beta-lactamase producing Pseudomonas aeruginosa strains isolated in hospitals in Recife, PE, Brazil

Cited by 30 publications

References 19 publications

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Pseudomonas aeruginosa infections: factors relating to mortality with emphasis on resistance pattern and antimicrobial treatment

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

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