Evaluation of bacteriocin-producing Lactobacillus sakei 1 against Listeria monocytogenes 1/2a growth and haemolytic activity

Martinez, Raquel Chacon Ruiz; Martinis, Elaine Cristina Pereira De

doi:10.1590/s1517-83822005000100016

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…Such inhibitory activities have been reported earlier in a numbers of LAB such as L. brevis and L. acidophilus L. sake , L. pentosus, etc. ( 32 , 33 ). The antibacterial potential of these bacteria has been attributed to its ability to produce H2O2, acids, phages, bacteriocins, and bacteriocin like substances ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Indigenous Lactobacillus Strains for Probiotic Properties

Mojgani

Hussaini²,

Vaseji³

2015

Jundishapur J Microbiol

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Background:Probiotics are defined as adequate amount of live microorganisms able to confer health benefits on the host. Currently, most commercially available probiotic products in the market belong to genera Lactobacillus. Traditional dairy products are usually rich source of Lactobacillus strains with significant health benefits. In order to evaluate the probiotic potential of these bacteria, it is essential to assess their health benefits, efficacy, and safety.Objectives:The probiotic efficacy of two Lactobacillus strains namely Lactobacillus pentosus LP05 and L. brevis LB32 was evaluated. They were previously isolated from ewes’ milk in a rural area in East Azerbaijan, Iran.Materials and Methods:The selected isolates were tested for certain phenotypic characters and identified to genus and species level by 16S rRNA gene sequencing and species specific primers. Further analysis included acid and bile resistance, antagonistic activity, cholesterol removing ability, survival in simulated gastric and upper intestine contents, aggregation and coaggregation properties. Finally, the adhering ability of the selected Lactobacillus strains to epithelial cells was tested using Caco-2 cell lines.Results:The selected isolates tolerated bile salt concentrations ranging from 0.5% to 3%, however their coefficient of inhibition were varied. Both isolates hydrolyzed bile and grew at pH values of 3, 4, and 5, while isolate LP05 was not able to hydrolyze arginine. Based on 16s rRNA gene sequencing and species-specific primers, the isolates were identified as L. brevis LB32 and L. pentosus LP05. In contrast to simulated gastric conditions, the growth rate of the isolates in alkaline conditions of upper intestine increased significantly with the passage of time reaching its maximum in 24 hours. These 2 isolates inhibited the growth of Listeria monocytogenes, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus, and Streptococcus pneumonia. Furthermore, L. brevis LB32 was able to reduce approximately 86% of cholesterol compared to L. pentosus LP05, which showed only 69% of reduction. Higher aggregation and coaggregation percentage and adherence to Caco-2 cell line was observed in L. pentosus LP05 compared to L. brevis LB32.Conclusions:This research study proved the presence of viable probiotic LAB microflora in the ewe milk with enhanced health benefits. The 2 selected Lactobacillus strains could be exploited in dairy or pharmaceutical industry in future.

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Section: Discussionmentioning

confidence: 99%

Characterization of Indigenous Lactobacillus Strains for Probiotic Properties

Mojgani

Hussaini²,

Vaseji³

2015

Jundishapur J Microbiol

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“…For the other resistant strain, the authors has reported a non-significant induction in the tested virulence genes [19]. In another study, exposing of L. monocytogenes to a sakacin 1 producing strain [21] showed no apparent effect of this class IIa bacteriocin on the hemolytic activity of L. monocytogenes [22]. Hence, more study is needed to have a clearer understanding on the relationship between class IIa bacteriocin resistance with virulence.…”

Section: Introductionmentioning

confidence: 98%

Global Transcriptional Analysis of Spontaneous Sakacin P-Resistant Mutant Strains of Listeria monocytogenes during Growth on Different Sugars

Tessema¹,

Møretrø²,

Snipen³

et al. 2011

PLoS ONE

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Subclass IIa bacteriocins have strong antilisterial activity and can control the growth of Listeria monocytogenes in food. However, L. monocytogenes may develop resistance towards such bacteriocins. In this follow-up study, the transcriptomes of a high level (L502-1) and a low level (L502-6) spontaneous sakacin P-resistant mutant strain of L. monocytogenes were compared to the wild-type (L502). The growth of the resistant strains was reduced on mannose but not affected on cellobiose and the transcriptomics was performed during growth on these sugars. The mannose phosphotransferase system (PTS) encoded by the mptACD operon (mpt) is known for transporting mannose and also act as a receptor to class IIa bacteriocins. The mpt was repressed in L502-1 and this is in accordance with abolition of the bacteriocin receptor with resistance to class IIa bacteriocins. In contrast, the mpt was induced in L502-6. Despite the induction of the mpt, L502-6 showed 1,000 times more resistance phenotype and reduced growth on mannose suggesting the mannose-PTS may not be functional in L502-6. The microarray data suggests the presence of other transcriptional responses that may be linked to the sakacin P resistance phenotype particularly in L502-6. Most of commonly regulated genes encode proteins involved in transport and energy metabolism. The resistant strains displayed shift in general carbon catabolite control possibly mediated by the mpt. Our data suggest that the resistant strains may have a reduced virulence potential. Growth sugar- and mutant-specific responses were also revealed. The two resistant strains also displayed difference in stability of the sakacin P resistance phenotype, growth in the presence of both the lytic bacteriophage P100 and activated charcoal. Taken together, the present study showed that a single time exposure to the class IIa bacteriocin sakacin P may elicit contrasting phenotypic and transcriptome responses in L. monocytogenes possibly through regulation of the mpt.

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“…sake, L. pentosusetc (7,24,25). The antibacterial potential of these bacteria havebeen attributed to their ability to produce H 2 O 2 , acids, phages, bacteriocins and bacteriocin like substances (26).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

Khanian¹,

Mojgani²,

Ahmedi³

2014

Int J Enteric Pathog

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Background:There is an increasing interest in search for antimicrobial peptides (bacteriocins and bacteriocin-like compounds) produced by lactic acid bacteria (LAB) because of their potential to be used as antimicrobial agents for improving the safety of food products. Objectives: The main objective of study was to evaluate the antibacterial potential of locally isolated Lactic Acid bacteria (LAB) and determine their bacteriocin producing ability in in-vitro conditions. Materials and Methods: The antibacterial activity of 77 isolated LAB strains was tested against a number of pathogens by well-diffusion method. The isolates demonstrating antimicrobial potential were selected and tested for the production of bacteriocin or bacteriocin like substance. The bacteriocin produced by two of the isolates were partially purified and characterized. Results:The results indicated the neutralized supernatant fluid of two of the isolates identified as L. brevis LB32 and L. pentosus LP05, were active against the growth of Listeria monocytogenes, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus and Streptococcus pneumoniae. Additionally, L. brevis LB32 was able to inhibit the growth of Salmonella typhi and Klebsiella pneumoniae, while, S. pnuemoniae and L. monocytogenes appeared to be the most sensitive strain as apparent by highest zone of inhibition against these pathogens, respectively. The antimicrobial activity in the supernatant fluids of the mentioned strains remained unaffected after treating with enzymes catalase, lipase and lysozyme, while were strongly sensitive to the action of proteolytic enzymes, suggesting the presence of bacteriocin like inhibitory substance (BLIS) in the two isolates. The inhibitory substance produced by the two isolates appeared heat resistant and tolerated 100˚C and 121˚C for 55 minutes and 20 minutes, respectively. Partial purification of the concentrated culture supernatant fluids of L. brevis LB32 and L. pentosus LP05 by ammonium sulphate (80%) and DEAE cellulose columns resulted in an enhanced activity (AU/mL) and yield. Using different pore size ultra filter membranes and SDS-PAGE analysis, the approximate molecular weight of the BLIS produced by L. brevis LB32 and, L. pentosus LP05 appeared to be approximately 4.5 and 6 KDa, respectively. In contrast to L. brevis LB32, L. pentosus LP05 harbored an 18Kb plasmid DNA which appeared to be carrying the bacteriocin gene as evident by plasmid curing experiments. All the mutants retained their host immunity and were resistant to the bacteriocin produced by the parent strain. Conclusions:In conclusion, the antibacterial activity possessed by these isolates might be used for the control of unwanted pathogens mainly in dairy products, and could be investigated further for using in fermented dairy products.

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Evaluation of bacteriocin-producing Lactobacillus sakei 1 against Listeria monocytogenes 1/2a growth and haemolytic activity

Cited by 11 publications

References 23 publications

Characterization of Indigenous Lactobacillus Strains for Probiotic Properties

Characterization of Indigenous Lactobacillus Strains for Probiotic Properties

Global Transcriptional Analysis of Spontaneous Sakacin P-Resistant Mutant Strains of Listeria monocytogenes during Growth on Different Sugars

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

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