Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

Phutela, Urmila Gupta; Dhuna, Vikram; Sandhu, Shobhna; Chadha, B. S.

doi:10.1590/s1517-83822005000100013

Cited by 109 publications

(77 citation statements)

References 24 publications

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“…Namasivavam et al (2011) working on B. cereus isolated from market solid waste reported that pectinase production was enhanced by wheat bran. Also, Phutela et al (2005) reported the enhancement of pectinase by wheat bran and industrial pectin on the production of pectinase from thermophilic Aspergilus fumigatus isolated from decomposing orange peels. In our study, optimum temperature between 50 and 60°C was obtained for the three Bacillus spp, with B. stearothermophilus showing highest activity at 60°C while B. cereus and B. subtilis both showed highest activities of pectinase at 50°C.…”

Section: Discussionmentioning

confidence: 99%

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A comparative study of pectinolytic enzyme production by Bacillus species

Torimiro¹,

Okonji²

2013

Afr. J. Biotechnol.

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The economic and ecological function of pectinase enzymes in industries is gaining much attention with the need of highly productive strains of microorganisms to reduce production cost. The present investigation is a comparative evaluation of Bacillus sp. pectinases. The three isolates isolated from agro-waste, Bacillus stearothermophilus, Bacillus cereus and Bacillus subtilis, showed very high pectinase activities with pectin as the substrate. The three isolates also showed varying degree of preference for banana peels and wheat bran but their activity towards orange peel was very low compared to other substrate. The effect of temperature on the pectinase of the three isolates showed that B. stearothermophilus had optimum temperature at 60°C while B. cereus and B. subtilis both showed optimum activity at 50°C. On the effect of pH, B. stearothermophilus, B. cereus and B. subtilis showed optimum pectinase activities at pH 7.5, 8.0 and 9.0, respectively. Metal ions enhanced the activity of pectinase produced by the three isolates. The study exhibited that B. stearothermophilus gave the most pectinase activity, optimum temperature and a moderate alkaline pH, possibly making it a better source for industrial purpose.

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Section: Discussionmentioning

confidence: 99%

“…Similarly, 35°C was reported for pectinase from Penicillin chrysogenum (Banu et al, 2010). Phutela et al (2005) reported an optimum temperature of 60°C for thermophilus A. fumigatus pectinase. Other thermophilic fungi have been reported to optimally produce enzymes at 50°C (Rubinder et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of pectinolytic enzyme production by Bacillus species

Torimiro¹,

Okonji²

2013

Afr. J. Biotechnol.

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“…Biotechnol., 7 (1): 13-22, 2015 (1%, w/v) as the sole source of carbon and screened for pectinolytic activity by a modified plate method of Phutela et al (2005). The sterilized and cooled medium (pH 6.5) was supplemented with norfloxacin at 300 mg/100 mL before pouring.…”

Section: Screening Of Fungal Isolates For Pectinase Activitymentioning

confidence: 99%

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Sethi¹,

Panda²,

Sahoo³

2014

Asian J. of Biotechnology

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In this study, Aspergillus terreus NCFT4269.10 was selected for the biosynthesis of pectinase after screening from fifteen native fungal strains as the performance of A. terreus was best in pectin hydrolyzing activity (2.75 cm). Aiming at the cost effective fermentation, several agro-industrial residues such as, Pearl Millet (PM), Finger Millet (FM), Orange Peels (OP), Mustard oil Cake (MoC) and Chikling Vetch Peels (CVP) were evaluated for highest pectinase production using Liquid Static Surface Fermentation (LSSF) and Liquid Shaking Fermentation (LShF). Among these substrates, orange peel was found to be the most suitable substrate for optimal pectinase biosynthesis (LSSF: 633.33±57.73 U mLG ) when the culture was grown under static and shaking condition at 30°C for 96 h. Maximum biomass was formed when OP was implemented for fermentation at static condition followed by CVP. But maximum biomass did not always support the enhanced pectinase biosynthesis in most of the cases. Partial purification of pectinase revealed a 3.86 fold purification and 27.63% yield of protein. Fermentation kinetics study revealed that purified enzyme activity yield per gram of substrate was maximum at both LSSF (1866.6 U gdsG 1 ) and LShF (2026.7 U gdsG 1 ) when biosynthesis of pectinase was carried out using OP. Ratio of enzyme yield and biomass were best with OP as compared to the other substrates both in static and shaking fermentation at crude and purified conditions.

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“…For being a saprophytic fungus, important to nutrient recycling, this fungus has already been isolated from different environments such as soil, compost and agricultural waste composting (Straatsma et al, 1994;Latgé, 2001;Phutela et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

FUNGOS ALERGÊNICOS E TOXIGÊNICOS NO COMPOSTO DE CULTIVO DE Agaricus brasiliensis

Dias¹,

Guimarães²,

Siqueira³

et al. 2009

RSA

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The mushrooms Agaricus bisporus and Agaricus brasiliensis are grown on substrate obtained through a composting process that involves a complex and little known microbial succession. Sporulating and rapid-growth ascomycetes, and in special Aspergillus fumigatus have been reported among the different species isolated from the A. bisporus compost, indicating that pathogenic or potentially toxigenic species can remain in the compost at the end of the pasteurization process. However, in Brazil, there are no reports of isolation and identification of fungi in the growing compost of A. brasiliensis mushrooms after the pasteurization process. This study aimed to evaluate, through serial dilution technique, the presence of contaminant fungi in two kinds of A. brasiliensis growing compost (1-sugar cane bagasse/coast-cross hay; 2-cotton residue/ coast-cross hay). Species of Aspergillus, Emericella and Penicillium genera have been identified. Toxigenic fungi, as Aspergillus ochraceus, Aspergillus terreus and Penicillium oxalicum, were isolated from the formulations of growing compost of A. brasiliensis. Aspergillus fumigatus was found in both formulations; however, an intense spore production on the surface was observed in compost produced from cotton residue; which caused its use to be non-viable, for safety reasons. Considering that A. fumigatus is a human pathogen, preventive measures should be taken by workers involved in the production of compost, in order to prevent successive inhalation of spores.Key-words: Composting; Aspergillus; aspergillosis; edible and medicinal mushroom. RESUMOOs cogumelos Agaricus bisporus e Agaricus brasiliensis são cultivados em substrato obtido por um processo de compostagem que envolve uma sucessão microbiana complexa e pouco conhecida. Dentre as diferentes espécies isoladas do composto de A. bisporus, foram relatadas espécies de ascomicetos esporulantes e de crescimento rápido e, em especial, Aspergillus fumigatus, indicando que espécies patogênicas ou potencialmente toxigênicas podem permanecer no composto ao final do processo de pasteurização. Entretanto, não há relatos no Brasil acerca do isolamento e identificação desses fungos a partir do composto de cultivo do cogumelo A. brasiliensis após o processo de pasteurização. Este estudo teve como objetivo avaliar, por meio da técnica de diluição seriada, a presença de fungos contaminantes em dois tipos de compostos de cultivo de Agaricus brasiliensis (1-bagaço de cana/capim Coast cross; 2-resíduo de algodão/capim coast-cross ). Foram identificadas espécies pertencentes aos gêneros Aspergillus, Emericella e Penicillium. Fungos toxigênicos como Aspergillus ochraceus, Aspergillus terreus e Penicillium oxalicum foram isolados a partir das formulações de composto de cultivo do cogumelo A. brasiliensis. Aspegillus fumigatus foi encontrado nas duas formulações, porém, no composto produzido com resíduo de algodão, verificou-se produção intensa de esporos na sua superfície, inviabilizando a sua utilização, por questões de segurança. Conside...

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Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

Cited by 109 publications

References 24 publications

A comparative study of pectinolytic enzyme production by Bacillus species

A comparative study of pectinolytic enzyme production by Bacillus species

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

FUNGOS ALERGÊNICOS E TOXIGÊNICOS NO COMPOSTO DE CULTIVO DE Agaricus brasiliensis

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