Phage amplification assay as rapid method for Salmonella detection

Siqueira, R. S. de; Dodd, Christine E. R.; Rees, Catherine

doi:10.1590/s1517-83822003000500040

Cited by 24 publications

(16 citation statements)

References 3 publications

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“…We hypothesize that P22 was not detected in St+Ph and St+Pb+Ph groups after 24 hours due to the eradication of Salmonella by the treatments. Since the results obtained by Siqueira et al 26 support the idea that bacteriophage detection is a good method for detecting Salmonella, it is possible that the absence of P22 indicates the absence of Salmonella in the present study. Consequently, such results indicate that P22 was able to eradicate ST from the intestines of birds treated with St+Ph since there was lack of microbiological recovery or PCR detection of either ST or P22.…”

Section: Discussionsupporting

confidence: 67%

Eradication of Salmonella Typhimurium in broiler chicks by combined use of P22 bacteriophage and probiotic

Marietto-Gonçalves

Lima²,

Donato

et al. 2011

Microbiol Res (Pavia)

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It has been reported that the phage therapy is effective in controlling the number of colony-forming unit (CFU) of Salmonella spp. in chicken gut. This paper describes the protective effect of phage and Lactobacilli administration on Salmonella infection in 1-day-old chicks. We administered the bacteriophage P22 in a single dose and a probiotic mixture of four species of bacteriocin-producing Lactobacillus once a day for one week. Samples were analyzed every 48 hours, and intestinal eradication of S. Typhimurium was confirmed after treatments. We observed an increase in the size of duodenal villi and cecal crypts, as well as an increase in body weight in groups that received daily doses of Lactobacilli. This study confirms the efficiency of bacteriophage therapy in controlling salmonellosis in chicks and the beneficial effect of Lactobacilli mixtures in the weight gain of the birds.

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Section: Discussionsupporting

confidence: 67%

Eradication of Salmonella Typhimurium in broiler chicks by combined use of P22 bacteriophage and probiotic

Marietto-Gonçalves

Lima²,

Donato

et al. 2011

Microbiol Res (Pavia)

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“…This method is based on the interactions of bacteriophage and their host bacteria coupled with inactivation of unadsorbed extracellular phages by phagicidal agent which can provide rapid and accurate detection of pathogens. A simple phage amplification assay in which phagicide was not used was reported as an alternative rapid method to detect Salmonella cells in chicken breast (De Siqueira et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a rapid microbial detection method via phage lytic amplification assay coupled with Live/Dead fluorochromic stains

Jassim

Griffiths

2007

Lett Appl Microbiol

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Aims: To develop a method for rapid detection of bacteria via bacteriophage amplification coupled with exogenous fluorochromic stains. Methods and Results: A method for the rapid detection of bacteria was developed which consisted of exposing the sample suspected to contain target cells to host‐specific phage. After at least one infection cycle, bacteria known to be infected by the phage (helper cells) were added and the number of nascent phage particles was estimated using the Live/Dead BacLight Bacterial Viability kit. Using Pseudomonas aeruginosa, it was shown that the dead helper cell population following phage infection was proportional to the initial number of target cells present in the original sample. Approximately 1 × 101 CFU per ml of P. aeruginosa could be detected within 4 h without the need for enrichment. Conclusions: The phage lytic amplification assay coupled with exogenous fluorochromic stains was able to detect approx. 1 × 101 CFU per ml of the target bacterium within 4 h. Significance and Impact of the Study: A method to detect low number of bacterial cells in a sample within 4 h without the need for enrichment was developed.

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“…Detection of fewer than five cells per milliliter was achieved with a 24-h assay. Favrin et al [40] also used phage amplification but with phage SJ2 to detect Salmonella at the low detection limit of three cells per 25 g -1 ground beef or per 25 mL -1 milk powder or chicken rinses in approximately 20 h. De Siqueira et al [71] used phage amplification with phage P22 to detect Salmonella in artificially contaminated chicken breasts at a limit of 10 4 CFU g -1 after 3-4 h of incubation. Using a magnetoelastic biosensor interface with immobilized phage E2, Lakshmanan et al [72] and Li et al [73] detected Salmonella Typhimurium in fat-free milk at 10 3 CFU mL -1 in 20 min and directly on the surface of whole tomato at 10 2 CFU mL -1 in 30 min.…”

Section: Detection Of Foodborne Pathogensmentioning

confidence: 99%

Pathogen detection using engineered bacteriophages

Smartt

Jegier

et al. 2011

Anal Bioanal Chem

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Bacteriophages, or phages, are bacterial viruses that can infect a broad or narrow range of host organisms. Knowing the host range of a phage allows it to be exploited in targeting various pathogens. Applying phages for the identification of microorganisms related to food and waterborne pathogens and pathogens of clinical significance to humans and animals has a long history, and there has to some extent been a recent revival in these applications as phages have become more extensively integrated into novel detection, identification, and monitoring technologies. Biotechnological and genetic engineering strategies applied to phages are responsible for some of these new methods, but even natural unmodified phages are widely applicable when paired with appropriate innovative detector platforms. This review highlights the use of phages as pathogen detector interfaces to provide the reader with an up-to-date inventory of phage-based biodetection strategies.

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Phage amplification assay as rapid method for Salmonella detection

Cited by 24 publications

References 3 publications

Eradication of Salmonella Typhimurium in broiler chicks by combined use of P22 bacteriophage and probiotic

Eradication of Salmonella Typhimurium in broiler chicks by combined use of P22 bacteriophage and probiotic

Evaluation of a rapid microbial detection method via phage lytic amplification assay coupled with Live/Dead fluorochromic stains

Pathogen detection using engineered bacteriophages

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