Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria

Oliveira, André Luiz Martinez de; Canuto, Erineudo de Lima; Reis, Verônica Massena; Baldani, José Ivo

doi:10.1590/s1517-83822003000500020

Cited by 49 publications

(43 citation statements)

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“…These five strains were previously tested and selected by Oliveira et al (2003Oliveira et al ( , 2006, in inoculation studies using the method described by Reis et al (1999). To obtain the inoculum, the bacteria (with initials BR) were obtained from the collection of diazotrophic bacteria at Embrapa Agrobiologia and grown in liquid DYGS culture medium (Silva et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Field studies showed the efficiency of the inoculant in increasing stalk and dry matter yield of different sugarcane varieties (Oliveira et al, 2003;Silva et al, 2009;Schultz et al, 2012). The benefits of inoculation with diazotrophic bacteria for the crop are not yet fully understood , however there are reports that the plantbacteria association may increase the contribution of atmospheric N 2 by biological fixation in plants, according to the cultivar and environmental conditions (Oliveira et al, 2003;Baldani et al, 2009). The application of these bacteria also induces other growthpromoting processes, e.g., the production of phytohormones, especially of auxins (Videira et al, 2012); phosphate solubilization (Singh et al, 2007;Shukla et al, 2008), solubilization of zinc compounds (Saravanan et al, 2007), increase in soil organic C content and increased N contents and retention of essential nutrients in the rhizosphere (Yadav et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Inoculation of sugarcane with diazotrophic bacteria

Schultz

Silva

Sousa

et al. 2014

Rev. Bras. Ciênc. Solo

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SUMMARYThe sugarcane industry, a strategic crop in Brazil, requires technological improvements in production efficiency to increase the crop energy balance. Among the various currently studied alternatives, inoculation with diazotrophic bacteria proved to be a technology with great potential. In this context, the efficiency of a mixture of bacterial inoculant was evaluated with regard to the agronomic performance and N nutrition of sugarcane. The experiment was carried out on an experimental field of Embrapa Agrobiologia, in Seropédica, Rio de Janeiro, using a randomized block, 2 × 3 factorial design (two varieties and three treatments) with four replications, totaling 24 plots. The varieties RB867515 and RB72454 were tested in treatments consisting of: inoculation with diazotrophic bacteria, N-fertilized control with 120 kg ha -1 N and absolute control (no inoculation and no N fertilizer). The inoculum was composed of five strains of five diazotrophic species. The yield, dry matter accumulation, total N in the shoot dry matter and the contribution of N by biological fixation were evaluated, using the natural 15 N abundance in non-inoculated sugarcane as reference. The bacterial inoculant increased the stalk yield of variety RB72454 similarly to fertilization with 120 kg ha -1 N in the harvests of plant-cane and first ratoon crops, however the contribution of biological N fixation was unchanged by(1) Part of the doctoral thesis of the first author, Post-graduation course in Agronomy -Soil Science (CPGA-CS) at the Rural Federal University of Rio de Janeiro -UFRRJ. Received for publication on January 16, 2013 and approved on January 6, 2014. (2) Post-graduate student at CPGA-CS, Soil Science Department, UFRRJ. BR 465, km 7. CEP 23890-000 Seropédica (RJ), Brazil. O aumento da eficiência produtiva da cana-de-açúcar com tecnologias que possibilitem aumentar seu balanço energético é uma necessidade do setor sucroenergético, por se tratar de uma cultura estratégica para o país. Entre as diversas alternativas atualmente estudadas, o inoculante com bactérias diazotróficas vem se evidenciando uma tecnologia com elevado potencial. Diante desse contexto, este estudo teve como objetivo avaliar a eficiência do inoculante bacteriano no desempenho agronômico e na nutrição nitrogenada da cana-de-açúcar. O ensaio foi realizado no campo experimental da Embrapa Agrobiologia, em Seropédica, RJ. O delineamento experimental foi em bloco ao acaso, em esquema fatorial 2 × 3 (duas variedades e três tratamentos), com quatro repetições, totalizando 24 parcelas. As variedades utilizadas foram a RB867515 e RB72454. Os tratamentos foram: inoculação com bactérias diazotróficas, controle nitrogenado com 120 kg ha -1 de N e controle absoluto (sem inoculação e sem N). O inoculante foi composto por cinco estirpes de cinco espécies de bactérias diazotróficas. As variáveis avaliadas foram a produtividade de colmos, o acúmulo de matéria seca e N total da parte aérea das plantas e a contribuição da fixação biológica de N (FBN), utilizando como referên...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inoculation of sugarcane with diazotrophic bacteria

Schultz

Silva

Sousa

et al. 2014

Rev. Bras. Ciênc. Solo

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“…The BNF contribution to sugarcane plants grown in the field for 18 months after inoculation at the micropropagated stage with a mixture of endophytic diazotrophic bacteria, was in the range of 20 to 30% of the total N accumulated in the plant tissues (Oliveira et al 2003). Other measurement studies using the delta 15 technique showed that the average BNF contribution to the sugarcane varieties harvested in sugarcane producing areas in Brazil is around 30% (Boddey et al 2001).…”

Section: Inoculation Responsementioning

confidence: 98%

History on the biological nitrogen fixation research in graminaceous plants: special emphasis on the Brazilian experience

Baldani

2005

An. Acad. Bras. Ciênc.

229

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This review covers the history on Biological Nitrogen Fixation (BNF) in Graminaceous plants grown in Brazil, and describes research progress made over the last 40 years, most of which was coordinated by Johanna Döbereiner. One notable accomplishment during this period was the discovery of several nitrogen-fixing bacteria such as the rhizospheric (Beijerinckia fluminensis and Azotobacter paspali), associative (Azospirillum lipoferum, A. brasilense, A. amazonense) and the endophytic (Herbaspirillum seropedicae, H. rubrisubalbicans, Gluconacetobacter diazotrophicus, Burkholderia brasilensis and B. tropica). The role of these diazotrophs in association with grasses, mainly with cereal plants, has been studied and a lot of progress has been achieved in the ecological, physiological, biochemical, and genetic aspects. The mechanisms of colonization and infection of the plant tissues are better understood, and the BNF contribution to the soil/plant system has been determined. Inoculation studies with diazotrophs showed that endophytic bacteria have a much higher BNF contribution potential than associative diazotrophs. In addition, it was found that the plant genotype influences the plant/bacteria association. Recent data suggest that more studies should be conducted on the endophytic association to strengthen the BNF potential. The ongoing genome sequencing programs: RIOGENE (Gluconacetobacter diazotrophicus) and GENOPAR (Herbaspirillum seropedicae) reflect the commitment to the BNF study in Brazil and should allow the country to continue in the forefront of research related to the BNF process in Graminaceous plants.

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“…Moreover, Oliveira et al (2003) found that the number of diazotrophic bacteria isolated from sugar cane tissue decreases in plants fertilized with high levels of N compared with the number of bacteria in plants treated with low doses of N-fertilizer. The determination of the nitrogen use efficiency and the balance of this element under inoculation conditions versus levels of N-fertilizer are required to determine how inoculation with diazotrophic bacteria contributes to nutrition of inoculated plants.…”

Section: Resultsmentioning

confidence: 99%

Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

et al. 2016

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The variance analysis showed significant effects (p < 0.05) of inoculation (Londrina environment) and N rates (both environments) only for grain yield. There was no inoculation effect in the grain yield when inoculants were applied together with N-fertilization at topdressing. In the absence of N-fertilization at topdressing, the inoculants Masterfix L. and UEL promoted higher grain yield as compared to the uninoculated plants, with resulting increases of 13.21 and 26.61% in yield, respectively.

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Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria

Cited by 49 publications

References 2 publications

Inoculation of sugarcane with diazotrophic bacteria

Inoculation of sugarcane with diazotrophic bacteria

History on the biological nitrogen fixation research in graminaceous plants: special emphasis on the Brazilian experience

Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

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