PCR detection of four periodontopathogens from subgingival clinical samples

Ávila-Campos, Mário Júlio

doi:10.1590/s1517-83822003000100017

Cited by 12 publications

(15 citation statements)

References 11 publications

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“…However, in Brazil, the oral occurrence of selected periodontal pathogens is still unclear. A study conducted by Avila-Campos (2003) (7) also demonstrated a high prevalence of A. actinomycetemcomitan (70%), P. gingivalis (66%) and F. nucleatum (92%) in the healthy population made up of subjects between 18-51 years of age and 10% of blacks. Nevertheless, considering that the disease is dependent of host characteristics, we cannot affirm that the bare presence of these microorganisms represents an additional risk factor to the outbreak of the disease in the future.…”

Section: Discussionmentioning

confidence: 84%

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Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Victor¹,

Cortelli²,

Aquino³

et al. 2008

Braz. J. Microbiol.

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This cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic subjects who live in Salvador/Bahia-Brazil and have never smoked. Periodontal Probing Depth (PPD), Clinical Attachment Level (CAL), Plaque Index (PI) and Gingival Index (GI) were measured and analyzed by ANOVA and Wilcoxon tests (p<0.05) according to gender and age. The presence of A.actinomycetemcomitans, P.gingivalis, E.corrodens and F.nucleatum was determined by PCR and was analyzed by ANOVA, Wilcoxon, Student-t tests (p<0.05). Mean values of PPD and CAL were 2.18 and 1.0mm, respectively. Clinical parameters did not show differences between subjects of varying gender and age. The microbial prevalence was observed to be 95.45% for E.corrodens followed by F.nucleatum with 68.18%, A.actinomycetemcomitans with 45.45% and P gingivalis with 40.9%. An association between the presence of pathogens and gender and age was not observed (p<0.05). PPD, CAL and PI were not associated with P.gingivalis; however, GI appeared in higher frequencies among subjects without P.gingivalis. In this young, black ethnic, Brazilian population, a high percentage (96.96%) of subjects harbored at least one selected periodontal pathogen, but most subjects showed a healthy periodontal status. Further investigations are required to evaluate the actual influence of the presence of these bacterial species.

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Section: Discussionmentioning

confidence: 84%

“…Additionally, these studies also show variations that are related to the presence of periodontal pathogens in populations from different countries (7,14,22,47). Methodological factors may partially justify these discrepancies because bias of information, memory or selection might interfere with the data development.…”

Section: Introductionmentioning

confidence: 79%

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Victor¹,

Cortelli²,

Aquino³

et al. 2008

Braz. J. Microbiol.

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“…Previous other studies evaluating the subgingival microbiota involved a considerable diversity of participants (8, 24, 25). Diversity in samples leads to a greater number of biases, but encourages the participation of a large number of individuals.…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, few studies have been conducted to investigate the periodontal microbiota, most of these being conducted in the State of São Paulo (7, 8, 9, 13, 14, 15, 26). In these investigations, the bacteria P. gingivalis had its prevalence ranging from 17.8% to 90%, T. forsythia 33.3% to 100%, A. actinomycetemcomitans 23% to 90% and T. denticola was only investigated by Avila-Campos and Velásquez-Meléndez (7) with 60% of positive samples.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of periodontal pathogens among patients with chronic periodontitis

Farias¹,

Souza²,

Ferreira³

et al. 2012

Braz. J. Microbiol.

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The aim of the present study was to evaluate the presence of the periodontal pathogens that form the red complex (Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola) and Aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. The sample consisted of 29 patients with a clinical and radiographic diagnosis of chronic periodontitis based on the criteria of the American Academy of Periodontology (3). Samples for microbiological analysis were collected from the four sites of greatest probing depth in each patient, totaling 116 samples. These samples were processed using conventional polymerase chain reaction, which achieved the following positive results: 46.6% for P. gingivalis, 41.4% for T. forsythia, 33.6% for T. denticola and 27.6% for A. actinomycetemcomitans. P. gingivalis and T. forsythia were more prevalent (p < 0.05) in periodontal pockets ≥ 8 mm. The combinations T. forsythia + P. gingivalis (23.2%) and T. forsythia + P. gingivalis + T. denticola (20.0%) were more frequent in sites with a probing depth ≥ 8 mm. Associations with the simultaneous presence of A. actinomycetemcomitans + P. gingivalis, A. actinomycetemcomitans + T. forsythia, P. gingivalis + T. forsythia and T. forsythia + T. denticola were statistically significant (p < 0.05). It was concluded that the red complex pathogens are related to chronic periodontitis, presenting a higher occurrence in deep periodontal pockets. Moreover, the simultaneous presence of these bacteria in deep sites suggests a symbiotic relationship between these virulent species, favoring, in this way, a further progression of periodontal disease.

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“…After centrifugation (14,000 g, 10 minutes), the supernatant was transferred to a new microcentrifuge tube and used as a control. 24 Primer sequences were obtained according to Ashimoto et al 25 (Table 1), from Invitrogen. Primers were resuspended in TE buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA pH 8.0) and stored at 220uC.…”

Section: Methodsmentioning

confidence: 99%

Periodontopathogens around the surface of mini-implants removed from orthodontic patients

Tortamano¹,

Dominguez²,

Haddad³

et al. 2012

The Angle Orthodontist

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Objective: To verify if mini-implant mobility is affected by the presence of periodontopathogens, frequently associated with peri-implantitis. Materials and Methods: The surfaces of 31 mini-implants used for skeletal anchorage in orthodontic patients were evaluated. Polymerase chain reaction was used for identification of the presence of DNA from three different periodontopathogens (P. intermedia [Pi ], A. actinomycetemcomitans [Aa], and P. gingivalis [Pg]) in 16 mini-implants without mobility (control group) and 15 mini-implants with mobility (experimental group). Results: The results showed that Pi was present in 100% of the samples, from both groups: Aa was found in 31.3% of the control group and in 13.3% of the experimental group. Pg was detected in 37.4% of the control group and in 33.3% of the experimental group. The Fisher exact test and the odds ratio (OR) values for Aa and Pg (OR 5 0.34; 95% confidence interval [CI]: 0.05-2.10 and OR 5 0.61; 95% CI: 0.13-2.79, respectively) showed no significant association (P . .05) between the periodontopathogens studied and the mobility of the mini-implants. Conclusions: It can be concluded that the presence of Aa, Pi, and Pg around mini-implants is not associated with mobility. (Angle Orthod. 2012;82:591-595.)

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PCR detection of four periodontopathogens from subgingival clinical samples

Cited by 12 publications

References 11 publications

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Occurrence of periodontal pathogens among patients with chronic periodontitis

Periodontopathogens around the surface of mini-implants removed from orthodontic patients

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