Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level

Delamare, Ana Paula Longaray; Artico, Liane de Oliveira; Grazziotin, Felipe G.; Echeverrigaray, Sérgio; Costa, Samuel Macedo

doi:10.1590/s1517-83822002000400016

Cited by 11 publications

(8 citation statements)

References 21 publications

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“…It was successfully used in a study of thermophilic bacteria from deep-sea hydrothermal field in the Pacific Ocean. It is regarded as a simple, rapid, inexpensive and reliable method, and can be applied as a routine laboratory assay (Delamare, Artico, Grazziotin, Echeverrigaray, & da Costa, 2002). In this study, the profile of whole cell protein of each strain usually had more than 15 bands.…”

Section: Discussionmentioning

confidence: 89%

A survey on occurrence of thermophilic bacilli in commercial milk powders in China

et al. 2012

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Section: Discussionmentioning

confidence: 89%

A survey on occurrence of thermophilic bacilli in commercial milk powders in China

et al. 2012

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“…Several studies have used RAPD-PCR technique for epidemiological investigation of different microorganisms [10]. The usefulness of RAPD technique in the detection of the relationship between isolate has been reported [29]. Szczuka and Kaznowski [11] documented that both RAPD and ERIC techniques are of value in the differentiation between unrelated strains.…”

Section: Resultsmentioning

confidence: 99%

Aeromonas hydrophila from Fish and Humans: Biofilm Formation and Genetic Relatedness

Mohamed

Ahmed

Rizk³

et al. 2019

Zagazig Veterinary Journal

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A comprehensive study was done to determine the genetic relatedness and the biofilm formation ability of Aeromonas hydrophila strains (n=25) isolated from fish (150 tilapia and 144 mugil) and human stool samples (n=102). The results revealed that Random Amplification of Polymorphic DNA (RAPD-PCR) classified A. hydrophila strains into seven distinct profiles (R1-R7), the amplicon sizes ranged from 183-2930 bp. The isolates were grouped into five main clusters, the presence of isolates from fish and humans in the same cluster indicates the possibility of cross contamination. Biofilm results showed that 96% of A. hydrophila isolates were biofilm producers. At 35ºC, 16 (64%) and 8 (32%) showed strong and moderate biofilm production ability, respectively. At 25ºC, 21 (84%) were biofilm producers, of which, 8 (32%), 7 (28%) and 6 (24%) were strong, moderate and weak, respectively. At 4ºC, decreased biofilm production ability was noticed 13 (52%), where 8 (32%) and 5 (20%) were moderate and weak biofilm producers, respectively. Significant correlation was showed between A. hydrophila isolates and different cultivation temperatures. This clarified the potential virulence of A. hydrophila isolates from both fish and human sources and their public health hazard.

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“…However, RAPD analysis distinguished the isolates from the C. violaceum MTCC 2656 type strain. These results indicate that the RAPD analysis in Chromobacterium isolates with the set of primers tested was able to differentiate at strain level (Delamare et al. 2002) but was not very useful in their identification.…”

Section: Discussionmentioning

confidence: 86%

Genetic diversity study of Chromobacterium violaceum isolated from Kolli hills by amplified ribosomal DNA restriction analysis (ARDRA) and random amplified polymorphic DNA (RAPD)

Ponnusamy

Jose²,

Savarimuthu³

et al. 2011

Letters in Applied Microbiology

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Aim: Chromobacterium are saprophytes that cause highly fatal opportunistic infections. Identification and strain differentiation were performed to identify the strain variability among the environmental samples. We have evaluated the suitability of individual and combined methods to detect the strain variations of the samples collected in different seasons. Methods and Results: Amplified ribosomal DNA restriction analysis (ARDRA) and random amplified polymorphic DNA (RAPD) profiles were obtained using four different restriction enzyme digestions (AluI, HaeIII, MspI and RsaI) and five random primers. A matrix of dice similarity coefficients was calculated and used to compare these restriction patterns. ARDRA showed rapid differentiation of strains based on 16S rDNA, but the combined RAPD and ARDRA gave a more reliable differentiation than when either of them was analysed individually. Conclusion: A high level of genetic diversity was observed, which indicates that the Kolli Hills’C. violaceum isolates would fall into at least three new clusters. Significance and Impact of the Study: Results showed a noteworthy bacterial variation and genetic diversity of C. violaceum in the unexplored, virgin forest area.

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Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level

Cited by 11 publications

References 21 publications

A survey on occurrence of thermophilic bacilli in commercial milk powders in China

A survey on occurrence of thermophilic bacilli in commercial milk powders in China

Aeromonas hydrophila from Fish and Humans: Biofilm Formation and Genetic Relatedness

Genetic diversity study of Chromobacterium violaceum isolated from Kolli hills by amplified ribosomal DNA restriction analysis (ARDRA) and random amplified polymorphic DNA (RAPD)

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