Woronin bodies in Penicillium janczewskii Zaleski

Pessoni, Rosemeire Aparecida Bom; Freshour, Glenn; Figueiredo-Ribeiro, Rita de Cássia L.; Hahn, Michael G.; Braga, Márcia R.

doi:10.1590/s1517-83822002000200005

Cited by 3 publications

(2 citation statements)

References 9 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimum pH lies in acidic range (4.5 & 5.5) for activity of inulinases I & II ( Fig. 8 & 9), this pH range was identical to that recorded for inulinase (4.5) from A. niger-245 (7), 5.0 from A. niger, (50), and P. janczewskii, 5.0 (32,33). As pH value diverged from the optimum level, the efficient functioning of the enzyme was affected, most probably, due to the change in active site conformation which is determined, in part, by ionic and hydrogen bonding that can be affected by pH.…”

Section: Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l

Fawzi¹

2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

Thirty fungal species grown on Cichorium intybus L. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. The thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 gave the highest production levels of inulinases I & II at 50 and 24 ºC respectively. Yeast extract and peptone were the best nitrogen sources for highest production of inulinases I & II at five and seven days of incubation respectively. The two inulinases I & II were purified to homogeneity by gel-filtration and ion-exchange chromatography with 66.0 and 42.0 fold of purification respectively. The optimum temperatures of purified inulinases I & II were 75 and 50 ºC respectively. Inulinase I was more thermostable than the other one. The optimum pH for activity was found to be 4.5 and 5.5 for inulinases I & II respectively. A comparatively lower Michaelis-Menten constant (2.15 mg/ml) and higher maximum initial velocity (115 µmol/min/mg of protein) for inulinase I on inulin demonstrated the exoinulinase's greater affinity for inulin substrate. These findings are significant for its potential industrial application. The molecular mass of the inulinases I & II were estimated to be 72 & 78 kDa respectively by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

show abstract

Section: Discussionsupporting

confidence: 76%

“…Inulin hydrolyzing enzymes (inulinases) are produced by plants, filamentous fungi, yeasts, and bacteria. Among the filamentous fungi, Aspergillus and Penicillium species are common inulinase producers (5,9,21,25,42,43 (14,28,32).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l

Fawzi¹

2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

Cell-wall structure and composition of Penicillium janczewskii as affected by inulin

et al. 2005

View full text Add to dashboard Cite

Penicillium janczewskii, a filamentous fungus isolated from the rhizosphere of Vernonia herbacea (Asteraceae), grows rapidly on media containing either sucrose or inulin as carbon sources. Maintenance of P. janczewskii on inulin medium induces secretion of proteins with high inulinase activity but results in a mycelium that easily collapses and breaks. We evaluated the influence of inulin on fungal growth and colony morphology and on cell-wall structure and composition in comparison with growth and wall characteristics on sucrose-containing medium. P. janczewskii grown on Czapek medium with agar containing 1% (w/v) sucrose or inulin showed differences in the color and morphology of the colonies, although growth rates were similar on both carbon sources. Scanning-electron microscopy revealed that the hyphae from fungus grown on inulin-containing medium are much thinner than those from fungus cultivated on sucrose. Ultrastructural analysis of 5 d old cultures using transmission-electron microscopy indicated significant differences in the cell-wall thickness between hyphae grown on inulin or sucrose media. No differences were detected in the overall carbohydrate and protein contents of cell walls isolated from cultures grown on the two carbon sources. Glycosyl composition analyses showed glucose and galactose as the predominant neutral monosaccharides in the walls but showed no differences attributable to the carbon source. Glycosyl linkage composition analyses indicated a predominance of 3-linked glucopyranosyl in the hyphal walls when P. janczewskii was grown on inulin-containing medium. Our results suggest that growth on inulin as the sole carbon source results in structural changes in the mycelia of P. janczewskii that lead to mycelial walls with altered physical and biological properties.

show abstract

Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sources

2007

View full text Add to dashboard Cite

Penicillium janczewskii, isolated from the rhizosphere of Vernonia herbacea, grows rapidly on media containing either sucrose or inulin, although inulin more than sucrose induced the production of inulinases. Three different extracellular beta-fructofuranosidases (two inulinases and one invertase) were purified from fungal cultures grown on sucrose or inulin, through precipitation with ammonium sulfate, and anion-exchange, hydrophobic interaction and gel filtration chromatographies. The optimum temperature of the three enzymes was approximately 60 C, optimum pH 4-5.5 and apparent molecular mass of 80 kDa. K(m) and V(max) values determined for invertase on sucrose were respectively 3.7 10(-4) M and 7.9 10(-2) micromol/min/mL, and on inulin 6.3 10(-2) M and 2.09 10(-2) micromol/min/mL. The values of k(m) for the two inulinases were 8.11 10(-4) and 2.62 10(-3) M, being lower for inulin when compared to those obtained for sucrose. The inulinases did not produce oligofructans from inulin, indicating they are primarily exoinulinases. The differences found in inulinase induction patterns when inulin or sucrose was used seem to be related to modifications on the enzyme properties, mainly concerning substrate affinity.

show abstract

Woronin bodies in Penicillium janczewskii Zaleski

Cited by 3 publications

References 9 publications

Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l

Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l

Cell-wall structure and composition of Penicillium janczewskii as affected by inulin

Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sources

Contact Info

Product

Resources

About