Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse

Rezende, Maria Inês; Barbosa-Dekker, Aneli M.; Vasconcelos, Ana Flora Dalberto; Endo, Asae Sakurada

doi:10.1590/s1517-83822002000100014

Cited by 48 publications

(30 citation statements)

References 22 publications

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“…Both zymograms using CMC and xylan as substrate showed a signal around 20 kDa (Figure 2, lanes 3 and 5). The weaker signal in the xylan zymogram indicates the presence of a~20 kDa xylanolytic activity which is compatible with other studies of this species that describe xylanase activities around this molecular mass with the lack of cellulase activity (Rezende et al 2002;Lee et al 2009;do Vale et al 2012). The L04 cellulase activity around 20 kDa could correspond to the endoglucanase (EGIII) from T. harzianum IOC3844 characterized by Generoso et al (2012) described with a low molecular mass, lack the cellulose binding domain (CBD) and able to degrade amorphous cellulose such as CMC.…”

Section: Discussionsupporting

confidence: 90%

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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Brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. For this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the Brazilian Cerrado. Among 13 isolates, a Trichoderma harzianum strain (L04) was identified as a promising candidate for cellulase production when cultured on in natura sugarcane bagasse. Strain L04 revealed a well-balanced cellulolytic complex, presenting fast kinetic production of endoglucanases, exoglucanases and β-glucosidases, achieving 4,022, U.L -1 (72 h), 1,228 U.L -1 (120 h) and 1,968 U.L -1 (48 h) as the highest activities, respectively. About 60% glucose yields were obtained from sugarcane bagasse after 18 hours hydrolysis. This new strain represents a potential candidate for on-site enzyme production using sugarcane bagasse as carbon source.

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Section: Discussionsupporting

confidence: 90%

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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“…These results agree with those reported by Rezende et al (2002), and Okafor et al (2007) where xylanase activity varied from 3.4 to 13 U (mg protein) -1 . These authors used supplemented agricultural by-products such as sawdust, sugarcane pulp, wheat bran, and oat spelt xylan as substrates using either T. harzianum or A. niger ANL 301.…”

Section: Effect Of Nutrient Supplementation On Optimization Of Xylanosupporting

confidence: 93%

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Regalado‐González¹,

Vázquez-Obregón²,

García-Almendárez³

et al. 2011

Electro Journal of Biotech

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Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2 9-5 , and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a DraperLin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein) -1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg -1 dry ACS for 12 hrs before exposure to cow's ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed.

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“…The xylanase production with all the lignocellulosics was found to increase upto 17 days of fermentation period, except with wheat and jowar straw where maximum activity was achieved on 14 th day of incubation period (Table 1). Rezende (2002) reported the plateau of xylanase activity from 5 th to 14 th day for T. harzianum using sugarcane bagasse as carbon source (11). Among the various carbon sources, maximum induction was achieved with xylan showing enough activity even at 7 day of incubation period and reached maximum by17 th day.…”

Section: Optimization Of Incubation Period and Medium Constituentsmentioning

confidence: 99%

Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

Goyal¹,

Kalra²,

Sareen³

et al. 2008

Braz. J. Microbiol.

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In the present study, cultural and nutritional conditions for enhanced production of xylanase by a local soil isolate of Trichoderma viride, using various lignocellulosic substrates in submerged culture fermentation have been optimized. Of the lignocellulosics used, maize straw was the best inducer followed by jowar straw for xylanase production. The highest activity achieved was between 14 to 17 days of fermentation. A continuous increase in xylanase production was observed with increasing level of lignocellulosics in the medium and highest activity was observed with maize straw at 5% level. Xylanase production with higher levels of lignocellulosics (3 to 5%) of maize, jowar and barseem was found to be higher as compared to that with commercial xylan as carbon source. Sodium nitrate was the best nitrogen source among the six sources used. Maximum xylanase production was achieved with initial medium pH of 3.5-4.0 and incubation temperature of 25ºC.The enzyme preparation was effective in bringing about saccharification of different lignocellulosics. The xylanase production could be further improved by using alkali treated straw as carbon source.

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Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse

Cited by 48 publications

References 22 publications

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

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