Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil

Scaletsky, Isabel C. A.; Fabbricotti, Sandra H.; Carvalho, Rozane de Lima Bigelli; Nunes, Claudia R.; Maranhão, Hélcio de Sousa; Morais, Mauro Batista de; Riley, Lee W.; Fagundes-Neto, Ulysses

doi:10.1590/s1517-83822001000400012

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…A stool specimen was collected from each subject and checked for bacterial and parasitic pathogens (Scaletsky et al, 2001(Scaletsky et al, , 2002 and rotavirus (Fernandes et al, 2000).…”

Section: Methodsmentioning

confidence: 99%

The epidemiological and clinical characteristics and nutritional development of infants with acute diarrhoea, in north–eastern Brazil

Maranhão¹,

Medeiros²,

Scaletsky³

et al. 2008

Annals of Tropical Medicine & Parasitology

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In order to assess the epidemiological and clinical characteristics and changing nutritional status of infants suffering from acute diarrhoea, 103 infants with such diarrhoea and the same number of age-matched controls were investigated at the Universidade Federal do Rio Grande do Norte's Paediatric Hospital, in north-eastern Brazil. Each child with diarrhoea was given oral rehydration or, in the severe cases, intravenous rehydration. Each subject was checked for enteropathogens and his or her weight, height and weight-for-height, weight-for-age and height-for-age Z-scores were evaluated immediately after any clinical dehydration had been corrected and 30 days later. In the infants aged <6 months, a diet that included foods other than breast milk (odds ratio=9.41), including one in which breast milk was supplemented with other foods (odds ratio=4.69), was found to be statistically associated with diarrhoea. The enteropathogens found most commonly in the children with diarrhoea were rotavirus (36.9%), enteropathogenic Escherichia coli (11.6%) and Shigella (11.6%). Just four (5.2%) of the 77 cases with adequate follow-up showed persistent diarrhoea. At presentation or as soon as any clinical dehydration had been corrected, the infants with diarrhoea had significantly lower weights and weight-for-height and weight-for-age Z-scores than the controls. Thirty days later, however, the weight-for-height and weight-for-age Z-scores of the cases had increased significantly, to the point when they were not significantly different from the baseline values for the controls. The negative consequences of diarrhoea on weight-for-height and weight-for-age Z-scores and the recovery of these parameters after 30 days with rehydration reflect the acute but reversible influence of diarrhoea on infant nutritional status.

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“…A stool specimen was collected from each subject and checked for bacterial and parasitic pathogens (Scaletsky et al, 2001(Scaletsky et al, , 2002 and rotavirus (Fernandes et al, 2000).…”

Section: Methodsmentioning

confidence: 99%

The epidemiological and clinical characteristics and nutritional development of infants with acute diarrhoea, in north–eastern Brazil

Maranhão¹,

Medeiros²,

Scaletsky³

et al. 2008

Annals of Tropical Medicine & Parasitology

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“…The strains that adhered to the monolayers were recorded as adhering in localized (LA), localized-like (LAL), diffuse (DA), or aggregative (AA) patterns. 33 All tests were repeated at least two times as the duplicates helped to eliminate the potential technical errors, the bacterial adherence was calculated for each preparation by the standard error of mean using SPSS 17 software.…”

Section: B Bacterial Culturementioning

confidence: 99%

“…A total of 10 representative E. coli strains (2 isolates from each adherent pathotypes) were selected based on their virulence factor, cell adhesion properties, and the drug-resistance pro le, which was subjected to bacterial quantitative adhesion assays on HeLa and CHO-k1 monolayer as per previously described protocols. 33 2. Cell lines and culture medium: HeLa and CHO-k1 were procured from National Centre for Cell Sciences (NCCS), Pune, India.…”

Section: B Bacterial Culturementioning

confidence: 99%

“Beneficial Role of Skim Milk Against Drug Resistant Escherichia Coli Associated With Pediatric Diarrhoea"

Chellapandi¹,

Subbarayan

Mandal

et al. 2021

Preprint

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Background: Antibiotic-resistance in E. coli is a global issue affecting humans especially the pediatric population. Antibiotic-resistant E. coli is a pathogen frequently pediatric population as well as healthy adults. Methods: This study aimed to examine the antibiotic resistance of E. coli causing pediatric diarrhea and its drug-resistant rates, its adhering abilities to cell line in vitro, and inhibition efficiency of a few selected chemical compounds. Clinical strains were isolated from both the healthy and infected pediatric population of Mizoram. Results: Adhesion is a significant pathogenic process during bacterial infections, which has been employed for pathotyping of DEC by comparing adhesion efficiency in both normal (CHO-k1) and cancer (HeLa) cell lines. E. coli adherent pathotypes were identified by both PCR assay and in-vitro cell adhesion assays; the study also evaluated the adhesion inhibition ability of human skimmed milk, gentamicin, and cephalexin in-vitro. Of all isolates, 20.05% of adherent DEC (EPEC, DAEC, and EIEC) and 11.39 % of non-adherent DEC (STEC and ETEC) were found to be associated with pediatric diarrhoea in Mizoram. Human skimmed milk has a high potential adhesion inhibition against EAEC (50.25/90.90 µg/mL), EPEC (53.42/259.70 µg/mL), and EIEC (59.13/30.30 µg/mL) in both cell lines in comparison with gentamicin and cephalexin. Conclusion: This study concludes that as a dietary supplement- human skimmed milk has high potential to prevent adhesion of DEC pathotypes in cells in-vitro thus in in-vivo.

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Beneficial role of skim milk against drug-resistant Escherichia coli associated with pediatric diarrhoea

Chellapandi

Subbarayan

Mandal

et al. 2022

New Microbes and New Infections

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Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil

Cited by 3 publications

References 36 publications

The epidemiological and clinical characteristics and nutritional development of infants with acute diarrhoea, in north–eastern Brazil

The epidemiological and clinical characteristics and nutritional development of infants with acute diarrhoea, in north–eastern Brazil

“Beneficial Role of Skim Milk Against Drug Resistant Escherichia Coli Associated With Pediatric Diarrhoea"

Beneficial role of skim milk against drug-resistant Escherichia coli associated with pediatric diarrhoea

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