Protection by Lactobacillus acidophilus UFV-H2B20 against experimental oral infection with Salmonella enterica subsp. enterica Ser. Typhimurium in gnotobiotic and conventional mice

Moura, Lilian Nobre; Neumann, E.; Vieira, Leda Quércia; Nicoli, Jacques R.

doi:10.1590/s1517-83822001000100015

Cited by 19 publications

(16 citation statements)

References 9 publications

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“…We demonstrated previously that monoassociation with L. delbrueckii UFV-H2b20 confers resistance to Escherichia coli and Salmonella enterica var. Typhimurium [5,11]. In the present work, we show that L. delbrueckii UFV-H2b20 can also protect mice against L. monocytogenes infection.…”

Section: Introductionsupporting

confidence: 62%

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Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection

Santos

Silva

et al. 2010

Med Microbiol Immunol

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In the present study, we investigated the protective effects of Lactobacillus delbrueckii UFV-H2b20 on the resistance to Listeria monocytogenes infection in gnotobiotic mice. Germfree mice or monoassociated mice were infected with L. monocytogenes, and the microbiological and immunological responses were evaluated after 1, 3, and 5 days of infection. Monoassociation with L. delbrueckii was capable of protecting mice against death caused by L. monocytogenes and induced a faster clearance of the bacteria in the liver, spleen, and peritoneal cavity at days 1, 3, and 5 post-infection. Also, monoassociated mice displayed less liver injury than germfree mice. The production of TNF-α in the serum, peritoneal cavity, and gut was augmented in monoassociated mice. Likewise, the levels of IFN-γ found on supernatants of spleen cells cultures were higher after the monoassociation. In addition, increased production of nitric oxide in peritoneal cell cultures supernatants and in serum was observed in mice that received L. delbrueckii. The monoassociation with L. delbrueckii induced higher production of IL-10 in the mucosal immune system. We conclude that monoassociation with L. delbrueckii UFV-H2b20 protects mice from death caused by L. monocytogenes infection by favoring effector responses while preventing their immunopathological consequences.

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Section: Introductionsupporting

confidence: 62%

“…bulgaricus UFV-H2b20 protects mice against infectious diseases [5,11]. The main objective in the present study was the examination of in vivo action of L. delbrueckii UFV-H2b20 and its ability to modulate immune responses and increase host resistance against an infectious microorganism.…”

Section: Discussionmentioning

confidence: 99%

Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection

Santos

Silva

et al. 2010

Med Microbiol Immunol

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“…Like other probiotics, such as S. boulardii (Rodrigues et al, 1996), Bifidobacterium longum (Silva et al, 2004) and Lactobacillus acidophilus (Moura et al, 2001), S. cerevisiae 905 was rapidly eliminated from the digestive tract of mammals harbouring a complex intestinal microbiota (2-3 days) and for this reason daily ingestion of these micro-organisms was necessary to maintain artificially high viable levels in the gastrointestinal ecosystem of conventional animals. On the other hand, its implantation was possible in germ-free animals using a single dose.…”

Section: Discussionmentioning

confidence: 99%

Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice

Martins

Rodrigues²,

Tiago

et al. 2007

Journal of Medical Microbiology

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Previous results in the laboratory of the authors showed that Saccharomyces cerevisiae strain 905, isolated during 'cachaç a' production, was able to colonize and survive in the gastrointestinal tract of germ-free and conventional mice, and to protect these animals against oral challenge with Salmonella enterica serotype Typhimurium or Clostridium difficile. In the present work, the effects of S. cerevisiae 905 on the translocation of Salm. Typhimurium (mesenteric lymph nodes, Peyer's patches, spleen, liver) as well as on the immune system (number of Kü pffer cells, immunoglobulin production, clearance of Escherichia coli B 41 ) were evaluated in gnotobiotic and/or conventional mice. The treatment with the yeast reduced significantly the translocation of Salm. Typhimurium to liver in gnotobiotic animals and to all the organs tested in conventional mice. The number of Kü pffer cells per 100 hepatocytes in liver was significantly higher (P<0.05) in yeast mono-associated mice (52.9±15.7) than in germ-free controls (38.1±9.0). Probably as a consequence, clearance of E. coli B 41 from the bloodstream was more efficient in yeast mono-associated animals when compared to germ-free mice. Higher levels (P<0.05) of secretory IgA in intestinal content and of IgA and IgM in serum were observed in yeast mono-associated mice when compared to germ-free group. Concluding, the protection against pathogenic bacteria observed in a previous study was probably due to a modulation of both local and systemic immunity of mice treated with S. cerevisiae 905. INTRODUCTIONProbiotics are defined as live micro-organisms that when administered in adequate amounts confer a health benefit to the host (Food and Agriculture Organization of the United Nations & World Health Organization, 2002). These microorganisms are widely used in pharmaceutical preparations and fermented dairy products. Lactobacilli and Bifidobacteria are typically found in probiotic products for humans, as well as some yeasts such as Saccharomyces boulardii.S. boulardii, a non-pathogenic yeast, was isolated from lychee fruit in Indochina and grows at the unusually high temperature of 37 uC (McFarland & Bernasconi, 1993). It has been used for the treatment of different types of diarrhoeal diseases, such as antibiotic-associated diarrhoea (Bartlett, 1992;McFarland et al., 1995;Surawicz, 2003), Clostridium difficile-associated intestinal disease (Elmer et al., 1999;Surawicz et al., 2000;Surawicz, 2003), traveller's diarrhoea (Scarpignato & Rampal, 1995) and diarrhoea in HIV-infected patients (Born et al., 1993).Many mechanisms of action have been proposed to explain S. boulardii protection and they have been summarized by et al., 2004). This leads to the question whether other strains of Saccharomyces, as well as S. boulardii, possess biotherapeutic properties. Brazil is well known for its rich biodiversity in terms of animal, vegetal and microbial species, and yeast with probiotic properties certainly could be screened from Brazilian environmental and agroindustrial sources. ...

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“…Studies carried out in our laboratory (13,21) and others (22,23) have shown that monoassociation of mice with lactic acid bacteria renders mice more resistant to infections. A highly significant delayed mortality was observed in germ-free C3H/He/Oujco mice monoassociated with L. acidophilus and infected with Salmonella enterica var.…”

Section: Discussionmentioning

confidence: 93%

Lactobacillus delbrueckii UFV-H2b20 induces type 1 cytokine production by mouse cells in vitro and in vivo

Neumann

Ramos

Santos

et al. 2009

Braz J Med Biol Res

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Lactobacillus delbrueckii UFV-H2b20 has been shown to increase clearance of bacteria injected into the blood of germ-free mice. Moreover, it induces the production of type 1 cytokines by human peripheral mononuclear cells. The objective of the present study was to investigate the production of inflammatory cytokines [interleukin-12 (IL-12 p40), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)] triggered in vitro by live, heat-killed or lysozyme-treated L. delbrueckii UFV-H2b20 and in vivo by a live preparation. Germ-free, L. delbrueckii-monoassociated and lipopolysaccharide (LPS)-resistant C3H/HeJ mice were used as experimental models. UFV-H2b20 induced the production of IL-12 p40 and TNF-α by peritoneal cells and IFN-γ by spleen cells from germ-free or monoassociated Swiss/NIH mice and LPS-hyporesponsive mice (around 40 ng/mL for IL-12 p40, 200 pg/mL for TNF-α and 10 ng/mL for IFN-γ). Heat treatment of L. delbrueckii did not affect the production of these cytokines. Lysozyme treatment decreased IL-12 p40 production by peritoneal cells from C3H/HeJ mice, but did not affect TNF-α production by these cells or IFN-γ production by spleen cells from the same mouse strain. TNF-α production by peritoneal cells from Swiss/NIH L. delbrueckii-monoassociated mice was inhibited by lysozyme treatment. When testing IL-12 p40 and IFN-γ levels in sera from germ-free or monoassociated Swiss/NIH mice systemically challenged with Escherichia coli we observed that IL-12 p40 was produced at marginally higher levels by monoassociated mice than by germ-free mice (40 vs 60 ng/mL), but IFN-γ was produced earlier and at higher levels by monoassociated mice (monoassociated 4 and 14 ng/mL 4 and 8 h after infection, germfree 0 and 7.5 ng/mL at the same times). These results show that L. delbrueckii UFV-H2b20 stimulates the production of type 1 cytokines in vitro and in vivo, therefore suggesting that L. delbrueckii might have adjuvant properties in infection in which these cytokines play a major role.

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Protection by Lactobacillus acidophilus UFV-H2B20 against experimental oral infection with Salmonella enterica subsp. enterica Ser. Typhimurium in gnotobiotic and conventional mice

Cited by 19 publications

References 9 publications

Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection

Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection

Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice

Lactobacillus delbrueckii UFV-H2b20 induces type 1 cytokine production by mouse cells in vitro and in vivo

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