The use of alpha-methyl-D-glucoside, a synthetic analogue of maltose, as inducer of amylase by Aspergillus sp in solid-state and submerged fermentations

Abstract

The use of α methyl-D-glucoside (αMG), a synthetic analogue of maltose, as carbon source and inducer of amylase synthesis to several species of Aspergillus was studied in submerged and solid-state fermentations. Among a group of ten species, A. tamarii, A. fumigatus and A. flavus were able to produce biomass and high specific amylolytic activity in submerged cultures containing αMG as the only carbon source. In solid state fermentation, the enrichment of basal wheat bran or corn cob medium with αMG increased up to 3 times the production of amylases. In both submerged and solid state fermentations, aMG was more effective inducer of amylases than maltose and starch.

Production and Catalytic Properties of Amylases fromLichtheimia ramosaandThermoascus aurantiacusby Solid-State Fermentation

“…Additionally, Kunamneni et al [24] reported that wheat bran was the best substrate for amylase production by the fungus Thermomyces lanuginosus . Moreira et al [25] demonstrated a higher amylase production when different Aspergillus species were cultivated on wheat bran, increasing the production of the enzyme by up to 10-fold compared to that obtained by growth on other residues.…”

“…Bhatti et al [32] reported the glucoamylase production (about 61.35 U/g of dry substrate) when cultivated in Fusarium solani by SSF using wheat bran as substrate. Moreira et al [25] obtained amylases by SSF using wheat bran as substrate with no additional carbon sources, achieving approximately 350, 240, and 210 U/g of dry substrate by the fungi Aspergillus flavus , Aspergillus fumigatus , and Aspergillus tamari , respectively. The authors reported a 1.5- to 2-fold increase in the production of amylases in wheat bran supplemented with various carbon sources.…”

Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil

“…Though amylase production was slightly stimulated by maltose, it was remarkably induced by the native starches. Some reports indicate that maltose is an amylase inducer, though it does not elicit a high amylase yield (Moreira et al, 2001). This is probably the result of the easy breakdown of maltose to glucose which in turn represses amylase production (Ray et al, 1996).…”

Influence of Dimethyl Sulfoxide on Extracellular Enzyme Production by Pleurotus ostreatus

“…Water-soluble inducers have been widely studied for enhancing the production of enzymes in bacterial and fungal systems (Moreira et al 2001, Janas et al 2002, Rajoka & Khan 2005. In contrast, less is known as to the induction activity of molecules with limited or very low water-solubility.…”