Production of mycotoxins by galactose oxidase producing Fusarium using different culture

Abstract

The original isolate of the galactose oxidase producing fungus Dactylium dendroides, and other five galactose oxidase producing Fusarium isolates were cultivated in different media and conditions, in order to evaluate the production of 11 mycotoxins, which are characteristic of the genus Fusarium: moniliformin, fusaric acid, deoxynivalenol, fusarenone-X, nivalenol, 3-acetyldeoxynivalenol, neosolaniol, zearalenol, zearalenone, acetyl T-2, and iso T-2. The toxicity of the culture extracts to Artemia salina larvae was tested.

Identification of deoxynivalenol, 3‐acetyldeoxynivalenol, and zearalenone in galactose oxidase‐producing isolates of Fusarium graminearum

“…In the current research, rice was used as a substrate since it is most efficiently used for mycotoxin production by Fusarium isolates in two previously shown conditions (PEREIRA and KEMMELMEIER 2000). The first condition consisted of a short culture period comprising 14 days and the chief aim was to verify if DON and 3-ADON production was benefited in this condition (GIMENO et al 1992).…”

In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen–cell interactions

“…10 FG can secrete galactose oxidase (GO) to oxidize galactose (Gal) or N-acetylgalactosamine (GalNAc), one of the most common glycans decorating on human epithelia. While oxidizing the terminal C6 hydroxy group of Gal or GalNAc to the corresponding aldehyde, hydrogen peroxide (H 2 O 2 ) is generated through the reduction of oxygen, 11,12 which coexists with the superoxide anion (cO 2− ), hydroxyl radical (cOH) and singlet oxygen ( 1 O 2 ) in biological systems, collectively referred to as reactive oxygen species (ROS). 13 Thus, quantitative visualization of the generation of ROS during the oxidation of Gal and GalNAc, especially on specic protein, can provide the key information to reveal and deal with the FG induced human skin infection.…”