Total lipids and fatty acids of strains of Metarhizium anisopliae

Abstract

Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var. majus were similar in percentages of total lipids. Tukey test for average of lipid content in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p= 1%) with the highest means being obtained for conidia for fatty acid C18:1 and for myclelia for fatty acid C18:2.

Thermotolerance of germlings and mycelium of the insect‐pathogenic fungus Metarhizium spp. and mycelial recovery after heat stress

“…isolates, however, is from 35 to 37 °C [9][10][11][12][13][14], with 40 °C being the highest temperature permitting growth [15]. Several physiological changes are associated with fungal germination and growth, such as degradation of trehalose from conidia [16] and changing the fatty-acids of cell-membranes from saturated (conidium) to unsaturated (mycelium), which increases membrane perme-ability [17][18][19]. Accordingly, these physiological changes from conidium to mycelium severely reduce the heat resistance.…”

Lipid formation and γ-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil

“…The methodology in an automated system like Bioscreen C proved to be appropriate for the liquid medium cultures, being rapid and automated, and providing good prior indication of the optimal environmental factors for growth of the filamentous fungi (26). Bioscreen C has been also used for other authors studying filamentous fungi (14,18). In this study, it was enable to select the best carbon and nitrogen sources.…”

Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: role of the glyoxylate cycle during insect pathogenesis

“…Germinating conidia of M. anisopliae and B. bassiana contained constitutive levels of ICL and germination was delayed in the presence of an ICL inhibitor, suggesting a role in conidial germination also described for Aspergillus fumigatus and Magnaporthe grisea (Ebel et al, 2006;Wang et al, 2003). The glyoxylate cycle plays a fundamental role in the utilization of C 2 compounds, possibly in the form of storage lipids or fatty acids present in the conidia, which can be up to 48 % of the conidial dry weight in Metarhizium (Pupin et al, 2000). The reserves of polyols and trehalose present in conidia of M. anisopliae and B. bassiana are not sufficient to generate the energy needed for germination (Hallsworth & Magan, 1994).…”