In vitro evaluation of the effects of the interaction between irrigating solutions, intracanal medication and Er:YAG laser in dentin permeability of the endodontic system

Abstract

The purpose of this study was to evaluate in vitro the effects of different associations between irrigating solutions (EDTA-T and citric acid), intracanal medicament (NDP), and Er:YAG laser irradiation on dentin permeability. Fifty-one extracted single-rooted teeth were instrumented and divided into seven groups. Groups GI and GII had final irrigation with a demineralizing solution only (EDTA-T and citric acid, respectively). Groups GIII and GIV had final irrigation with EDTA-T and citric acid, respectively, plus an association of irrigating solution and Er:YAG laser. Groups GV and GVI had final irrigation with EDTA-T and citric acid, respectively, plus an association of intracanal medication and Er:YAG laser. Group GVII (control group) had final irrigation with distilled water. All root canals were filled with NDP associated with rhodamine B dye. After the experimental period, the samples were transversely cut into six 2.0 mm thick slices for subsequent reading using the ImageLab software. Analysis of the results allowed us to conclude that there were statistically significant differences (p < 0.05) between the groups as to the penetration of the dye-intracanal medication solution. Groups III and IV presented smaller values of dentinal permeability when compared to the other groups. The best results were obtained with the interaction between a demineralizing irrigating solution and the association of intracanal medicament and laser Er:YAG (groups V and VI). In these groups the observed penetration of the intracanal medicament plus dye solution in the apical third was, on average, 29% greater than in the other groups.

Analysis of Permeability and Morphology of Root Canal Dentin After Er,Cr:YSGG Laser Irradiation

“…The dye solution used to test dentin permeability was Rhodamine B, dissolved in a solution of the intracanal medication NDP (dexamethasone phosphate, paramonochlorophenol, and polyethyleneglycol; Fó rmula e Açã o Pharmacy, Sã o Paulo, SP, Brazil) at a concentration of approximately 20%. 17 The root canals were filled with the dye combined with NDP solution up to 1 mm from the apical foramen with a hypodermic syringe. After 20 seconds, the solution was removed by means of suction cannulae and absorbent paper.…”

Avaliação In vitro da irradiação com laser de Nd:YAG e Er:YAG no selamento radicular, variando-se o cimento obturador do sistemas de canais radiculares

“…Sabe-se que esse laser causa ablação dentinária, exposição dos túbulos e limpeza superficial da dentina. Essa topografia superficial da dentina favoreceria, BRUGNERA Jr. et al, 2003;PECORA et al, 2000) ou mesmo a diminuição da permeabilidade (ARANHA et al, 2005;RALD;LAGE-MARQUES, 2003). A observação dos grupos não irradiados mostrando que todos os espécimes do G 3 (Epiphany SE®) foram contaminados no período avaliado, é um indicador que a utilização deste cimento sem um prévio tratamento da superfície da dentina interna, o torna altamente frágil e não confiável em relação ao selamento lateral.…”

"Predição do risco de metástase do carcinoma bem diferenciado da glândula tireóide pela quantificação digital da imunoexpressão da galectina-3 nos compartimentos do tireócito maligno"

“…Desde então, uma série de pesquisas têm utilizado o método para análise morfométrica e quantificação da imunoexpressão de marcadores biológicos [110][111][112][113][114][115][116][117][118][119][120] . Abdo-Banhos…”