Yeasts from the oral cavity of children with AIDS: exoenzyme production and antifungal resistance

Bosco, Vera Lúcia; Birman, Esther Goldenberg; Cury, Arlete Emily; Paula, Claudete Rodrigues

doi:10.1590/s1517-74912003000300004

Cited by 32 publications

(33 citation statements)

References 10 publications

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“…In addition, two strains (8.3%) were very strong for phospholipase enzyme activity (Pz<0.69) and ten strains (41.7%) were strong (0.7<Pz <0.79). These results agree well with those obtained by others (Bosco et al 2003). On the other hand, 100% phospholipase-positive isolates were found elsewhere (de Oliveira et al 1998).…”

Section: Discussionsupporting

confidence: 94%

Biofilm formation and virulence properties of Candida spp. isolated from hospitalised patients in Tunisia

Chaieb

Eddouzi²,

Souiden³

et al. 2010

Ann Microbiol

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Control of opportunistic yeast is one of the factors reducing nosocomial infection in hospital. In this study, we investigated the presence of virulence factors including antifungal susceptibility, haemolytic potency, proteinase and phospholipase production. In addition biofilm formation was assessed using the XTT assay. A total of 24 Candida strains were isolated and identified. The most common species were Candida albicans and Candida glabrata (40.7%), followed by Candida dubliniensis (8.3%), Candida kefyr (4.2%) and Candida krusei (4.2%). In addition, 62.5% of strains were resistant to itraconazole and 16.7% of strains were resistant to fluconazole. All the strains were susceptible to amphotericin B. Phospholipase activity showed that 8.3% of the total strains were very strong producers and 41.7% were strong producers. Furthermore, three strains (12.5%) were found to have very strong proteinase activity. All the tested strains revealed beta haemolytic potency. All Candida strains were adhesive to polystyrene 96-well microtitre plates to differing degrees. The OD values of XTT reduction at 492 nm ranged from 0.025 (ca12) to 0.927 (ca2). The majority of C. albicans strains were highly metabolically active in comparison with the other species. Control of fungal infections in hospitalised patients is important to prevent the emergence of multi-resistant strains to commonly used antifungal agents.

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Section: Discussionsupporting

confidence: 94%

Biofilm formation and virulence properties of Candida spp. isolated from hospitalised patients in Tunisia

Chaieb

Eddouzi²,

Souiden³

et al. 2010

Ann Microbiol

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“…The results presented here should be interpreted with caution because only one single ATCC strain and three clinical HIV isolates of each Candida species were used. As strain variations in adherence, hydrophobicity and enzyme production have been documented, 40–46 higher numbers of isolates should be used in the future when testing interspecies differences in adhesion.…”

Section: Discussionmentioning

confidence: 99%

Growth of Candida species on complete dentures: effect of microwave disinfection

Sanitá

Vergani

Giampaolo

et al. 2009

Mycoses

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Microwave disinfection of complete dentures has been recommended to treat denture stomatitis in non-immune compromised patients. Oral candidiasis is a frequent manifestation of HIV infection. The objective of this study is to evaluate the effectiveness of microwave irradiation on the disinfection of complete dentures inoculated with American Type Culture Collection (ATCC) and HIV isolates of five species of Candida. Fifty dentures were made, sterilised and inoculated with the tested microorganisms (C. albicans, C. dubliniensis, C. krusei, C. glabrata and C. tropicalis). After incubation (37 degrees C/48 h), dentures were microwaved (650 W/3 min). Non-irradiated dentures were used as positive controls. Replicate aliquots of suspensions were plated at dilutions 10(-1) to 10(-4) and incubated (37 degrees C/48 h). Colony counts (cfu ml(-1)) were quantified. Dentures were also incubated at 37 degrees C for 7 days. Data were analysed with 2-way ANOVA and Tukey HSD tests (alpha = 0.05). Dentures contaminated with all Candida species showed sterilisation after microwave irradiation. All control dentures showed microbial growth on the plates. The cfu ml(-1) for C. glabrata was higher than those of C. albicans, C. dubliniensis and C. tropicalis whereas the cfu ml(-1) for C. krusei was lower. The cfu ml(-1) for clinical isolates was higher than those of ATCC yeast. Microwave irradiation for 3 min at 650 W resulted in sterilisation of all complete dentures.

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“…Microbiological studies in HIV‐positive children have focused mainly on species of Candida 1,2,12,13,23 . Regarding oral bacteria, Castro et al 9 .…”

Section: Discussionmentioning

confidence: 99%

Salivary microbiota of HIV‐positive children and its correlation with HIV status, oral diseases, and total secretory IgA

Silva-Boghossian

Castro

Teles³

et al. 2007

Int J Paed Dentistry

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Yeasts from the oral cavity of children with AIDS: exoenzyme production and antifungal resistance

Cited by 32 publications

References 10 publications

Biofilm formation and virulence properties of Candida spp. isolated from hospitalised patients in Tunisia

Biofilm formation and virulence properties of Candida spp. isolated from hospitalised patients in Tunisia

Growth of Candida species on complete dentures: effect of microwave disinfection

Salivary microbiota of HIV‐positive children and its correlation with HIV status, oral diseases, and total secretory IgA

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