Development and control of caries lesions on the occlusal surface using a new in vivo caries model

Paim, Susana; Modesto, Adriana; Cury, Jaime Aparecido; Thylstrup, A.

doi:10.1590/s1517-74912003000200017

Cited by 7 publications

(4 citation statements)

References 18 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo models have been previously tested for caries development [26,27] but, to our knowledge, this is the first study to use this model to investigate the biological shift during both process of enamel caries lesion progression and arrest (by including brushing), combining bacterial microbiome, metabolome, and enamel structure analyses. The design of the study was also planned to mimic the most realistic scenario.…”

Section: Discussionmentioning

confidence: 99%

“…Caries lesions initiation, progression and arrest on the premolars were induced for a maximum of eight weeks using modified orthodontic bands, i.e. with a gap on the smooth (buccal) surface, and a mesh on the occlusal surface to enable biofilm accumulation, as previously described [25][26][27] (Figures 1A and 1B). The teeth were extracted according to a timeline that allowed either four or six weeks of undisturbed biofilm accumulation for enamel caries lesion progression followed by a two-week mitigation period (via toothbrushing with fluoridated toothpaste) for caries lesion arrest.…”

Section: Caries Initiation Progression and Arrest Protocolmentioning

confidence: 99%

See 1 more Smart Citation

The bacterial microbiome and metabolome in caries progression and arrest

Rosa

Neves

Azcárate-Peril

et al. 2021

Journal of Oral Microbiology

View full text Add to dashboard Cite

Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the cariesinducing protocol for 4-and 6-weeks, and subsequently promoted caries lesion arrest via a 2week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Caries Initiation Progression and Arrest Protocolmentioning

confidence: 99%

The bacterial microbiome and metabolome in caries progression and arrest

Rosa

Neves

Azcárate-Peril

et al. 2021

Journal of Oral Microbiology

View full text Add to dashboard Cite

show abstract

“…However, for the assessment of the clinical activity status of occlusal surfaces, current concepts seem to be applied only to a certain extent. Improvement in this assessment is warranted as caries lesion activity is essential for diagnosis and subsequent treatment decisions in daily practice [Paim et al, 2003;Carvalho et al, 2016]. The general rule for any treatment decision related to caries activity is that in case of doubt, the lesion should be reassessed in short intervals.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis, Risk Assessment, and Treatment Decisions for Occlusal Caries: A Survey from the Danish Public Dental Health Service

Carvalho¹,

Qvist²,

Aimée³

et al. 2017

Caries Res

View full text Add to dashboard Cite

This study validates a case-based survey method and analyzes the extent to which Danish dental professionals apply current concepts and strategies for occlusal caries management in children, adolescents, and young adults. A case-based, precoded questionnaire consisting of 10 clinical cases/patients with 26 teeth/occlusal surfaces was developed. The cases were set up in a PowerPoint presentation and color printed as a booklet illustrating patients with different patterns of caries activity, severity, and risk. A total of 69 dental professionals participated. Content and face validity of the survey method was established using a panel of experts. The panel also assessed the reliability of the method using a test-retest procedure (κ ≥ 0.80) and acting as benchmark. Measurements of agreement between dental professional and benchmark assessments showed substantial agreement for overall caries activity and risk assessment of patients and for clinical and radiographic severity of occlusal lesions (κ = 0.61-0.67). For assessment of caries lesion activity on occlusal surfaces, the agreement was moderate (κ = 0.50). Regarding treatment decisions, dental professionals showed substantial agreement when indicating restorative treatments (κ = 0.68). Multivariate logistic regression analysis showed a significant influence of various patient-, lesion-, and participant-related variables in the assessment of caries risk, caries activity and severity, and treatment decision. In conclusion, Danish dental professionals participating in the study apply reasonably well current concepts on overall caries activity and risk assessment, clinical and radiographic severity of occlusal lesions, and, to a certain extent, assessment of caries lesion activity on occlusal surfaces. Nonoperative treatment decisions had a high priority among Danish professionals.

show abstract

“…At the 2-y follow-up, we found that regular toothbrushing with fluoride toothpaste 1.100 ppm F – diminished the risk of developing caries lesions in sound surfaces. Per se, the mechanical disturbance of the occlusal biofilm carried out on a regular basis was shown, in experimental clinical studies, to either maintain the caries process at subclinical levels or inactivate the process at a clinical level (Holmen et al 1988; Thylstrup et al 1994; Paim et al 2003). The combination of mechanical biofilm control and fluoride toothpaste is considered significant for management of the caries process in terms of reducing tooth mineral loss and enhancing remineralization.…”

Section: Discussionmentioning

confidence: 99%

Visible Occlusal Plaque Index Predicting Caries Lesion Activity

Carvalho¹,

Mestrinho

Aimée

et al. 2022

J Dent Res

View full text Add to dashboard Cite

This study was undertaken to appraise the predictive validity of the Visible Occlusal Plaque Index (VOPI) in assessing occlusal caries lesion activity in adolescents. A total of 618 adolescents aged 10 to 15 y were examined at the beginning of the trial and 511 (82.7%) at the 2-y follow-up. Adolescents and parents answered questionnaires about demographics, oral health behavior, and family sociodemographic variables. The VOPI has a 4-point ordinal scale ranging from no plaque to heavy plaque. Molar teeth were assigned to group VOPI 0–1 (no/thin plaque; n = 2,539) and group VOPI 2–3 (thick/heavy plaque; n = 843). At baseline examination, occlusal surfaces at risk of transition ( n = 3,382) were either sound (55%), inactive noncavitated lesions (21%), inactive cavitated lesions (1%), active noncavitated lesions (15%), or active cavitated lesions (7%). The relative risk (RR) for caries lesion transition was estimated. Sound occlusal surfaces with no or thin plaque were significantly more likely to remain sound (RR = 1.3; 95% confidence interval [CI]: 1.1–1.4) than those harboring thick or heavy plaque. Inactive noncavitated occlusal lesions presenting no or thin plaque were significantly less likely to progress to active noncavitated lesions (RR = 0.1; CI: 0.0–0.8) than their counterparts with thick or heavy plaque. Active noncavitated lesions harboring thick and heavy plaque had a significantly lowest chance of becoming sound (RR = 0.7; CI: 0.5–0.9) and a highest risk of remaining active (RR = 1.5; CI: 1.1–1.9). Stepwise logistic regression analyses were run according to surface status at baseline and showed that none of the nonclinical predictors were significant for the outcome. The presence of thick and heavy plaque on occlusal surfaces was a predictor for caries lesion development, progression and activity ( P < 0.0001). In conclusion, besides being an additional clinical tool for oral hygiene assessment, the VOPI is a predictor for development, progression, and activity of occlusal caries lesion. This is of particular interest for assessment of occlusal lesions undergoing health-promoting transitions.

show abstract

Development and control of caries lesions on the occlusal surface using a new in vivo caries model

Cited by 7 publications

References 18 publications

The bacterial microbiome and metabolome in caries progression and arrest

The bacterial microbiome and metabolome in caries progression and arrest

Diagnosis, Risk Assessment, and Treatment Decisions for Occlusal Caries: A Survey from the Danish Public Dental Health Service

Visible Occlusal Plaque Index Predicting Caries Lesion Activity

Contact Info

Product

Resources

About