Estudo morfológico da polpa de molares de ratos Wistar frente a uma oclusão traumática experimental

Penna, Luiz Alberto Plácido; Rode, Sigmar de Mello

doi:10.1590/s1517-74912000000200011

Cited by 3 publications

(5 citation statements)

References 9 publications

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“…The use of rats in the experimental model presents advantages such as straightforwardness in the handling and in the reproduction, and mainly, the easiness for controlling, predicting 22 , and standardizing 6 . Moreover, this model is better accepted regarding ethical and economic concerns 9 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an experimental rat model for comparative studies of bleaching agents

et al. 2016

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Dental materials in general are tested in different animal models prior to the clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents, by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats’ vital teeth.Material and Methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals were untreated (control). The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated through histomorphometric analysis with inflammatory cell count in the coronal and radicular thirds of the pulp. Fibroblasts were also counted. Scores were attributed to odontoblastic layer and vascular changes. Tertiary dentin area and pulp chamber central area were measured histomorphometrically. Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05).Results After 2 days, the amount of inflammatory cells increased in the coronal pulp occlusal third up to the 15-min application groups of each bleaching gel. In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, reduction on the pulp chamber central area and enlargement of the tertiary dentin area were observed, without the detection of inflammation areas.Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.

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Section: Discussionmentioning

confidence: 99%

Evaluation of an experimental rat model for comparative studies of bleaching agents

et al. 2016

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“…These results show that traumatic occlusion associated with previous subluxation caused a significant proliferation of inflammatory cells, showing a relation with the onset and progression of a pulp and peri radicular inflammation ( Yu, 2004 ) and further contributing to the occurrence of pulp sequelae such as odontoblast layer disorganization, pulp dystrophy, proliferation of collagen fibers, pulp calcification and a decreased volume of the pulp chamber ( Penna and Rode, 2000 , Popescu et al, 2011 ). The occurrence of injury to the apical neurovascular bundle following trauma promotes protein denaturation, characterized by water loss, where the pulp cells are kept in place in their framework, but coagulated and lifeless ( Consolaro, 2007 , Giovanini et al, 2011 ).…”

Section: Discussionmentioning

confidence: 97%

“…Pulp alterations lead to a doubtful prognosis for teeth subjected to subluxation ( Pedrini et al, 2018 , Pugliesi et al, 2004 ), which may be aggravated by factors like delayed treatment ( Pedrini et al, 2018 ), lack of containment ( Kahler and Heithersay, 2008 ), root canal infection ( Hermann et al, 2012 ) and traumatic occlusion ( Nakatsu et al, 2014 ). However, traumatic occlusion is still treated as a background variable, perhaps because it is considered a minor injury in dental trauma ( Caviedes-Bucheli et al, 2011 , Kvinnsland and Heyeraas, 1992 , Penna and Rode, 2000 , Popescu et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…Traumatic occlusion is another mechanical tissue injury that damage to the periodontal ligament ( Amaral et al, 2017 ), that may be related to the initiation and progression of pulp and periradicular inflammation ( Yu, 2004 ). In these cases, pulp sequelae can occur through neuroinflammation ( Caviedes-Bucheli et al, 2011 , Kvinnsland and Heyeraas, 1992 ), inhibition of pre-dentin formation ( Penna and Rode, 2000 , Stenvik and Mjör, 1971 ), vascular component lesions ( Stenvik and Mjör, 1971 ), odontoblast layer disorganization, pulp dystrophies, pulp calcifications, and decreased pulp chamber volume ( Penna and Rode, 2000 , Popescu et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation

Amaral,

Casatti,

Naqvi

et al. 2024

The Saudi Dental Journal

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“…Assim, para amenizar os artefatos produzidos na polpa, decorridos da descalcificação da estrutura dentária, se tem buscado novos protocolos de processamento, como o uso de energia de microondas associado ao EDTA e a separação da polpa por meio de clivagem (RODE; FARIA; MONTEIRO, 1996; PEN-NA; RODE, 2000). O uso da técnica de clivagem do dente visa à remoção prévia da polpa, eliminando, portanto, a etapa de descalcificação dos tecidos duros dentários.…”

Section: Palavras-chaveunclassified

Estudo Comparativo da Morfologia da Polpa Dentária de Dentes Humanos Submetidos à Técnica de Clivagem e a Técnicas de Descalcificação Química

Bernardi

Bento

Mattuella

et al. 2007

R. Fac. Odontol. Porto Alegre

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Os rápidos avanços do conhecimento acerca do reparo e regeneração tecidual, têm despertado o interesse pela biologia pulpar. Entretanto, para avaliar microscopicamente a dinâmica do tecido pulpar, é necessário, inicialmente, que o dente seja submetido aos processamentos histológicos de fixação e descalcificação. A descalcificação pode afetar o grau de coloração e pode causar desnaturação de proteínas. Além disso, é um processo demorado, visto que o dente requer um longo período de desmineralização. Assim, a proposta deste trabalho foi de avaliar qualitativamente a matriz extracelular e as células da polpa dentária, comparando três grupos: dois em que o tecido dentário foi descalcificado e um em que a polpa foi removida dos tecidos duros, não necessitando do processo de descalcificação. Dez pré-molares foram fixados em formalina tamponada a 10% por 24 horas. Após, estes dentes foram divididos em três grupos: 4 dentes foram submetidos a processo de descalcificação por meio de solução de Morse, 3 por meio de solução de EDTA a 10% e; os 3 dentes restantes tiveram sua polpa separada dos tecidos duros dentários por meio da técnica de clivagem. Na seqüência, os três grupos foram processados por meio da técnica histológica de rotina e foram corados com H/E. Os resultados desta análise demonstraram que houve uma melhor conservação tanto da matriz extracelular, quanto das estruturas celulares no grupo da clivagem, seguido do grupo Morse e por fim, com a menor conservação das estruturas pelo grupo EDTA

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Estudo morfológico da polpa de molares de ratos Wistar frente a uma oclusão traumática experimental

Cited by 3 publications

References 9 publications

Evaluation of an experimental rat model for comparative studies of bleaching agents

Evaluation of an experimental rat model for comparative studies of bleaching agents

Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation

Estudo Comparativo da Morfologia da Polpa Dentária de Dentes Humanos Submetidos à Técnica de Clivagem e a Técnicas de Descalcificação Química

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