Obtaining and characterization of bacterial cellulose synthesized by Komagataeibacter hansenii from alternative sources of nitrogen and carbon

Abstract

This work aimed to obtain and characterize bacterial cellulose (BC) membranes obtained by cultivating Komagataeibacter hansenii ATCC 23769 using mannitol, glucose, fructose, lactose, glycerol, inulin, and sucrose as carbon sources, and corn steep liquor and Prodex Lac® as alternative sources of nitrogen. The formation of the BC´s gelatinous membrane was monitored for 12 days under static conditions and a temperature of 30 ºC. After purification, the membranes were dried and characterized by thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The highest BC concentrations were found in the culture medium containing Prodex Lac® as the source of nitrogen. Among sugars, fructose and mannitol presented the best results. TGA analyzes indicate that all membranes have similar thermal behavior. The FTIR results show that the chemically synthesized membranes are equivalent to the structures cited in the literature. The micrographs obtained by SEM showed that the medium might influence BC´s morphology, but in general, all presented nanofibers, an essential feature in the membrane. Thus, the BC membranes synthesized in this study proved that the BC production using low-cost alternative means is feasible. The material obtained meets the expected thermal, physical, and chemical properties.

Bacterial Cellulose as a Versatile Biomaterial for Wound Dressing Application

“…BC is predominantly produced from C sources, mainly being glucose. However, other carbohydrate sources have also been reported, such as fructose [ 76 ], sucrose [ 77 ], galactose [ 78 ], corn steep liquor [ 77 ], and agro-industrial by products [ 79 , 80 ]. This biopolymer is produced via a progression of microbial enzymatic reactions, where the conversion of sugars into dextrose happens and then occurs their polymerization into cellulosic chains [ 81 ].…”

Production of High-Value-Added Bacterial Nanocellulose with Thinner Fiber Using Novel Yarrowia lipolytica Extract from Erythritol Industry Waste

“…BNC synthesis is a complex process affected by various factors, and efficient BNC production may depend on the efficient utilization of carbon and nitrogen sources. 18 Nitrogen, as an essential nutrient, plays a promoting role in microbial growth and cell construction. 19 In the process of BNC production, Saccharomyces cerevisiae yeast extract and peptone are commonly used nitrogen sources in BNC production.…”

“…However, most of these waste materials replaced carbon sources, and there are few alternatives to expensive nitrogen sources. BNC synthesis is a complex process affected by various factors, and efficient BNC production may depend on the efficient utilization of carbon and nitrogen sources . Nitrogen, as an essential nutrient, plays a promoting role in microbial growth and cell construction .…”