Contrastive research on the waterproof and dustproof mechanism of wild silkworm silk and domestic silkworm silk

Chen, Rouxi

doi:10.1590/s1517-70762014000400011

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…The silk quality can be correlated to mulberry (Morus spp.) cultivation processes, such as climatic conditions, spacing of cultivation, water availability for the plants, soil types, branches pruning, leaf growth, among others [3,4].…”

Section: Introductionmentioning

confidence: 99%

Effect of pyraclostrobin on mulberry leaves nutrients, silkworm cocoon production and silk fiber performance

et al. 2021

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The present work evaluates the effect of pyraclostrobin fungicide on the nutritional quality of mulberry leaves, silkworm cocoon production and silk fiber mechanical performance. The evaluation was performed in two consecutive years. In Spring 2015, mulberry plants were treated twice with 4 doses of pyraclostrobin (0, 100, 200, and 300 g.ha-1), 30 and 15 days before pruning of the branches. In Spring 2016, the same plants received the same doses of pyraclostrobin only 30 days before pruning of the branches. Fifth instar caterpillars were fed on treated mulberry leaves. The mineral content of mulberry leaves was determined on pruning day of the branches and 30 days before. The consumption of mulberry leaves and the mortality rate of the caterpillars fed with the treated leaves was verified, and the cocoons obtained were classified and weighed. Silk fibers were submitted to mechanical evaluation by tensile test. Morphological and physical modification were evaluated by Scanning Electron Microscope (SEM) and X-ray diffraction (XRD). In 2015, the mortality of caterpillars fed on leaves treated with pyraclostrobin was at least 26.7% higher than the control. At 2016 even with a single application the weight of the cocoons was lower. The main variation in the mineral content was observed in Mn and Fe nutrients. Mineral intake had a noticeable effect in both crystallinity and tensile strength. Results show that the use of pyraclostrobin may reduce the mechanical behavior of silk thread up to 70%, indicating that its use is not indicated for mulberry planting aiming silk feeding.

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Section: Introductionmentioning

confidence: 99%

Effect of pyraclostrobin on mulberry leaves nutrients, silkworm cocoon production and silk fiber performance

et al. 2021

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“…Thus, studying the fibroin differences between B. mori and A. pernyi silk is a basis for identifying their species. In the past several decades, various studies regarding silk characterization have been carried out using a variety of methods, such as scanning electron microscopy (SEM) 7 , Fourier transform infrared spectroscopy (FTIR) 8–10 and amino acid analysis 11 . These methods can distinguish well-preserved silk from other fibers and identify what species produced the silk.…”

Section: Introductionmentioning

confidence: 99%

Species identification of Bombyx mori and Antheraea pernyi silk via immunology and proteomics

Chen

et al. 2019

Sci Rep

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In recent years, increasing attention has been paid to the origin, transmission and communication of silk. However, this is still an unsolved mystery in archaeology. The identification of silk-producing species, especially silk produced by Bombyx mori ( B. mori ) and Antheraea pernyi ( A. pernyi ), is of key significance to address this challenge. In this study, two innovative methods, i.e. immunology and proteomics, were proposed and successfully established for the species identification of silks. ELISAs result demonstrated that the two prepared antibodies exhibited high sensitivity and specificity in distinguishing B. mori and A. pernyi silk. No cross-reactivity with each other was observed. Moreover, biomarkers were obtained for Bombyx and Antheraea through proteomic analysis. It was also confirmed that the biomarkers were suitable for identifying the species that produced a given silk sample. Compared with conventional methods for distinguishing silk species, immunological and proteomics techniques used in tandem can provide intact information and have the potential to provide accurate and reliable information for species identification.

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Discerning Silk Produced by Bombyx mori from Those Produced by Wild Species Using an Enzyme-Linked Immunosorbent Assay Combined with Conventional Methods

You

Zheng

et al. 2017

J. Agric. Food Chem.

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Recently, much interest has been paid to the separation of silk produced by Bombyx mori from silk produced by other species and tracing the beginnings of silk cultivation from wild silk exploitation. In this paper, significant differences between silks from Bombyx mori and other species were found by microscopy and spectroscopy, such as morphology, secondary structure, and amino acid composition. For further accurate identification, a diagnostic antibody was designed by comparing the peptide sequences of silks produced by Bombyx mori and other species. The results of the noncompetitive indirect enzyme-linked immunosorbent assay (ELISA) indicated that the antibody that showed good sensitivity and high specificity can definitely discern silk produced by Bombyx mori from silk produced by wild species. Thus, the antibody-based immunoassay has the potential to be a powerful tool for tracing the beginnings of silk cultivation. In addition, combining the sensitive, specific, and convenient ELISA technology with other conventional methods can provide more in-depth and accurate information for species identification.

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Contrastive research on the waterproof and dustproof mechanism of wild silkworm silk and domestic silkworm silk

Cited by 5 publications

References 9 publications

Effect of pyraclostrobin on mulberry leaves nutrients, silkworm cocoon production and silk fiber performance

Effect of pyraclostrobin on mulberry leaves nutrients, silkworm cocoon production and silk fiber performance

Species identification of Bombyx mori and Antheraea pernyi silk via immunology and proteomics

Discerning Silk Produced by Bombyx mori from Those Produced by Wild Species Using an Enzyme-Linked Immunosorbent Assay Combined with Conventional Methods

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