Cell adhesion on different titanium-coated surfaces

Resende, Cristiane Xavier; Lima, I.; Gemelli, Enori; Granjeiro, José Mauro; Soares, Glória Dulce de Almeida

doi:10.1590/s1517-70762010000200041

Cited by 6 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Then, 2 × 10 5 MC3T3-E1 cells were added into each well with the disc. According to a previous report [ 34 ], we adopted a 24-h time point. After 24 h, each disc was rinsed by PBS 3 times to remove the unattached cells and then incubated in 0.25 mg/mL (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide) (MTT) (Sigma-Aldrich, St. Louis, MO, USA) in PBS solution at 37 °C and 5% CO 2 [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Titanium Surfaces Created by 3D Printing Methods, Particle Sizes, and Acid Etching on Protein Adsorption and Cell Adhesion, Proliferation, and Differentiation

et al. 2022

View full text Add to dashboard Cite

The surfaces of 3D printed titanium prostheses have major impacts on the clinical performance of the prostheses. To investigate the surface effects of the products generated by 3D printed titanium on osseointegration, six surface types of titanium discs produced by the direct metal laser sintering (DMLS) and electron beam melting (EBM) methods, with two sizes of titanium particles and post-printing acid etching, were used to examine the surface topography and to explore the protein adsorption, pro-inflammatory cytokine gene expressions, and MC3T3-E1 cell adhesion, proliferation, and differentiation. The EBM-printed disc showed a stripy and smooth surface without evidence of the particles used, while the DMLS surface contained many particles. After acid etching, small particles on the DMLS surface were removed, whereas the large particles were left. Moreover, distinct proteins with low molecular weights were attached to the 3D printed titanium discs but not to the pre-printing titanium particles. The small titanium particles stimulated the highest TNF-α and IL-6 gene expressions at 24 h. The alizarin red content and osteocalcin gene expression at day 21 were the highest in the groups of acid-etched discs printed by DMLS with the small particles and by EBM. Therefore, the acid-treated surfaces without particles favor osteogenic differentiation. The surface design of 3D printed titanium prostheses should be based on their clinical applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of Different Titanium Surfaces Created by 3D Printing Methods, Particle Sizes, and Acid Etching on Protein Adsorption and Cell Adhesion, Proliferation, and Differentiation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…MEV-Obteve-se hidroxiapatita (rota A) e brushita (rota C) representando a topografia propícia a biofuncionalidade (figura 1) [1][2][3][4]. MET-Pode ser visto claramente que as partículas de HA preparadas pela Via A são com formato de agulhas com comprimento superior a 100 nm, mas largura de ~ 20 nm.…”

Section: Resultsunclassified

“…A. 1 , Campos, M. F. 1 , Fonseca, G. S. 1 1 Programa de Pós-Graduação em Engenharia Metalúrgica de Volta redonda-PPGEM\UFF 2 Programa de Pós-Graduação em Engenharia Metalúrgica e de Materiais-COPPE\UFRJ…”

unclassified

Síntese, caracterização por microscopia eletrônica e modelagem de nano-pós de HA e brushita obtidas a partir de recursos renováveis

Lima¹,

Fialho²,

Simão³

et al. 2023

Anais Do Simpósio De Engenharia Metalúrgica E De Materiais Sul Fluminense

View full text Add to dashboard Cite

“…The osteoblast cells at the tissue/implant interface use proteins to adhere to the surface of the titanium alloy and produce an ECM with a characteristic composition [8]. The various factors that influence the response of this interface including surface morphology, pH, ionic composition, strength of the solution, functional groups of proteins and the Ti alloy substrate which determine protein adsorption must be well understood since the adhesion and subsequent proliferation of osteoblast cells on different Ti-based alloys has been shown to be altered substantially by the topography and the composition of the materials used [9–12]. …”

Section: Introductionmentioning

confidence: 99%

Electrochemical characterization of MC3T3-E1 cells cultured on γTiAl and Ti–6Al–4V alloys

Bueno-Vera

Torres-Zapata

Sundaram

et al. 2015

Bioelectrochemistry

View full text Add to dashboard Cite

Electrochemical Impedance Spectroscopy (EIS) was used to study the behavior of MC3T3-E1cells cultured in αMEM+FBS solution on two Ti-based alloys (Ti-6Al-4V and γTiAl) during 4, 7 and 14 days. EIS measurements were carried out at the open-circuit potential in the 1 mHz to 100 kHz frequency range. Results indicate a general increase in impedance on the Ti alloy surfaces with cells as a function of time. Bode plots indicate changes corresponding to the passive oxide film, adsorption of proteins and cell tissue on surfaces with the passage of time. Normal cellular activity based on the polygonal morphology, with long and fine cytoplasmic prolongations of the cells on Ti-6Al-4V and γTiAl was observed from SEM images. Similarly, mineralization nodules corresponding to cell differentiation associated with the osseogenetic process were observed confirmed by Alizarin Red S staining. Immunofluorescence analysis to detect the presence of collagen Type I showed an increase in the segregation of collagen as a function of time. The impedance values obtained from EIS testing are indicative of the corrosion protection offered to the Ti alloy substrates by the cell layer. This study shows that γTiAl has better corrosion resistance than Ti-6Al-4V in the αMEM+FBS environment in the presence of MC3T3-E1 cells.

show abstract

Cell adhesion on different titanium-coated surfaces

Cited by 6 publications

References 17 publications

Effects of Different Titanium Surfaces Created by 3D Printing Methods, Particle Sizes, and Acid Etching on Protein Adsorption and Cell Adhesion, Proliferation, and Differentiation

Effects of Different Titanium Surfaces Created by 3D Printing Methods, Particle Sizes, and Acid Etching on Protein Adsorption and Cell Adhesion, Proliferation, and Differentiation

Síntese, caracterização por microscopia eletrônica e modelagem de nano-pós de HA e brushita obtidas a partir de recursos renováveis

Electrochemical characterization of MC3T3-E1 cells cultured on γTiAl and Ti–6Al–4V alloys

Contact Info

Product

Resources

About