Validação de método analítico espectrofotométrico UV para determinação de ácido úsnico em lipossomas

Siqueira-Moura, Marigilson P.; Lira, Mariane C. B.; Santos-Magalhães, Nereide Stela

doi:10.1590/s1516-93322008000400008

Cited by 13 publications

(7 citation statements)

References 10 publications

(10 reference statements)

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“…In this sense, derivative spectrophotometric method with detection in UV is low cost and is easily applied to analysis of raw materials and pharmaceuticals, as well as in kinetic studies of drug release, detection of analyte in the presence of decomposition compounds, and many other applications. 18 Derivative spectrophotometry (DS) is based on so called derivative spectra which are generated from conventional or zero-order scan. The derivatization of zero-order spectrum can lead to the separation of overlapped signals and elimination of background caused by presence of other compounds in a sample without previous separation or purification.…”

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confidence: 99%

Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

et al. 2012

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Recebido em 28/2/11; aceito em 13/6/11; publicado na web em 8/8/11A derivative spectrophotometric method was validated for quantification of acyclovir in poly (n-butylcyanoacrylate) (PBCA) nanoparticles. Specificity, linearity, precision, accuracy, recovery, detection (LOD) and quantification (LOQ) limits were established for method validation. First-derivative at 295.2 nm eliminated interferences from nanoparticle ingredients and presented linearity for acyclovir concentrations ranging from 1.25 to 40.0 µg/mL (r = 0.9999). Precision and accuracy data demonstrated good reproducibility. Recovery ranged from 99.3 to 101.2. LOD was 0.08 µg/mL and LOQ, 0.25 µg/mL. Thus, the proposed method proved to be easy, low cost, and accurate, and therefore, an useful alternative to quantify acyclovir in nanoparticles.

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confidence: 99%

Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

et al. 2012

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“…In the literature, the quantification of hydrophobic molecules in inclusion complexes and liposomes have been performed successfully using the proposed spectrophotometry method (Siqueira-Moura et al, 2008;Cavalcanti et al, 2012;Lapenda et al, 2012).…”

Section: Application Of the Method: Determination Of Lpsf/ac04 In Incmentioning

confidence: 99%

“…Despite the clear advantages of using the HPLC chromatographic technique, the method has several limitations such as the high cost of instrumentation and operation, relatively long analysis times and the need for experience in handling the equipment and in processing samples (Siqueira-Moura et al, 2008). Spectrophotometry is a highly convenient analytical technique that is widely used in laboratories for quality control given its simplicity, low cost and wide availability (Darwish et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

Ferraz

Mendonça

Silva

et al. 2015

Braz. J. Pharm. Sci.

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The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL −1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL -1 ] + 0.00348), (r 2 =0.9995). The limits of detection and quantification were 0.047µg.mL −1 and 0.143µg.mL −1 , respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than 5% and no statistically significant difference between theoretical and practical concentrations was detected. Thus, a rapid, simple, low cost and sensitive spectrophotometric method was developed and validated for determining the content of inclusion complex and liposomes containing LPSF/AC04. Uniterms:UV Spectrophotometry/quantitative analysis/method validation. Acridine derivatives/ determination. Inclusion complex. Liposomes.O objetivo deste estudo foi desenvolver e validar um método espectrofotométrico para determinação do LPSF/AC04 em complexo de inclusão e encapsulado em lipossomas. Os parâmetros de validação foram determinados de acordo com o International Conference on Harmonisation (ICH) e Agência Nacional de Vigilância Sanitária (ANVISA). OLPSF/AC04 foi determinado a 250 nm em metanol pelo método espectrofotométrico UV, que apresenta linearidade na faixa de 0,3 a 2 µg/mL (Absorbância = 0,18068 x [LPSF/AC04 µg/mL] + 0,00348), (r 2 = 0,9995). Os limites de detecção e quantificação foi 0,047 µg/mL e 0,143 µg/mL, respectivamente. O método foi exato, preciso, reprodutível e robusto e todas as amostras analisadas apresentaram coeficiente de variação menor que 5% e não houve diferença estatisticamente significante entre a concentração teórica e a prática. Assim, um método espectrofotométrico rápido, simples, sensível e de baixo custo foi desenvolvido e validado para determinar o conteúdo do LPSF/ AC04 em complexos de inclusão e encapsulados em lipossomas.Unitermos: Espectrofotometria UV/análise quantitativa/validação de método. Derivados acridínicos/ determinação. Complexos de inclusão. Lipossomas.

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“…A promising alternative related to the incorporation of new drugs on the market (Ribeiro-Costa et al 2004, 2009, Santos et al 2006, Siqueira-Moura et al 2008, Grumezescu et al 2014, Martinelli et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats

Marinho

Antonio

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to evaluate the teratogenic and hepatotoxic potential of the usnic acid encapsulated into PLGA-microspheres. In total, 12 female Wistar rats in pregnancy were randomly distributed in the control group (n= 6) that received 1.0 mL of physiological solution and treatment group (n= 6) that received 25 mg/kg of encapsulated usnic acid by oral administration. All females were euthanized at day 20 of pregnancy and their fetuses were removed and analyzed. During the pregnancy was observed a reduction in weight gain. There was no difference in serum transaminases levels analyzed as well as any difference in liver weight in both groups. The histomorphometric analysis of the liver from the treatment group revealed an increase in number of hepatocytes and a decrease in nuclear area of these cells. Moreover, no alteration was observed in cell area of hepatocytes or number of Kupffer cells. The fetuses had an increase in total number of hepatocytes and a reduction in the amount of megakaryocytes. These results show the hepatotoxic potential of usnic acid during pregnancy. However, its toxicity can be minimized by encapsulation in microspheres.

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Validação de método analítico espectrofotométrico UV para determinação de ácido úsnico em lipossomas

Abstract: O ácido úsnico (AU) é um composto de origem liquênica e tem demonstrado importantes atividades biológicas

Cited by 13 publications

References 10 publications

Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats

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