Cyclosporin A in skin samples from in vitro penetration studies may be assayed by a simple HPLC method

Lopes, Luciana B.; Bentley, Maria Vitória Lopes Badra

doi:10.1590/s1516-93322005000400010

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References 22 publications

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“…The recovery percentage of quercetin extracted from skin layers (SC + [E + D]) and only [E + D] was showed in Table I. By comparing the results obtained by Lopes and Bentley (2005) in terms of cyclosporine recovery from skin layers, the use of the same methodologies was more efficient in the recovery of quercetin. Thus, these high recoveries obtained for different concentrations demonstrate that the methods for quercetin extraction and quantification in different skin layers, described in the present study, were very suitable to be employed on cutaneous penetration studies.…”

Section: Resultsmentioning

confidence: 99%

Assessment of in vitro methodologies to determine topical and transdermal delivery of the flavonoid quercetin

Vicentini

Georgetti

Bentley

et al. 2009

Braz. J. Pharm. Sci.

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To be effective against the oxidative damages induced by UVB irradiation in the skin, the drug needs to release from the formulation in which it was incorporated and reach the skin layers where the ROS are generated. Thus, it is very important the development of a robust and sensitive methodology to extract and quantify in different skin layers the antioxidant agent delivered from topical formulations. Therefore, in the present work suitable methods to extract and quantify quercetin in skin samples and receptor phase after in vitro penetration studies were developed. The results demonstrated that the recovery from two different layers of skin, the SC and [E+D], using two different methods of quantification (DPPH• assay and HPLC, respectively), was 93.8 % when the quercetin spiked dose was 50 µg/mL, 100.4 % when it was 100 µg/mL and 89.9 % for 250 µg/mL and the average recovery of the quercetin extraction from receptor phase when dichloromethane was used as extractor solvent was 96%. These results demonstrate that the described methods have a potential application to in vitro skin penetration studies of quercetin, since it showed to be accurate and sensitive.
Para ser efetiva contra os danos oxidativos induzidos pela radiação UVB na pele, é necessário que o ativo seja liberado da formulação na qual foi incorporado e alcance as camadas da pele onde são geradas as EROS. Desta forma, torna-se de grande importância o desenvolvimento de métodos eficazes e sensíveis para extrair e quantificar, nas diferentes camadas de pele, o agente antioxidante liberado de formulações tópicas. No presente trabalho foram desenvolvidos métodos adequados para extrair e quantificar a quercetina em amostras de pele e na fase receptora após estudos de penetração cutânea in vitro. Os resultados demonstraram que a recuperação das camadas de pele, EC e [E+D], quando do uso de duas diferentes metodologias de quantificação (ensaio de DPPH• e CLAE, respectivamente), foi de 93,8 % quando aplicada uma dose de 50 µg/mL de quercetina, 100,4 % para 100 µg/mL e 89,9 % para 250 µg/mL e a recuperação média da extração da quercetina da fase receptora, quando do emprego de diclorometano como solvente extrator, foi de 96 %. Tais resultados demonstram que os métodos descritos têm grande potencial de aplicação em estudos de penetração in vitro já que apresentaram exatidão e sensibilidade

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Section: Resultsmentioning

confidence: 99%

Assessment of in vitro methodologies to determine topical and transdermal delivery of the flavonoid quercetin

Vicentini

Georgetti

Bentley

et al. 2009

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

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Cyclosporin A in skin samples from in vitro penetration studies may be assayed by a simple HPLC method

Abstract: (1, 10 and 20 g/mL). The coefficients of variation and deviation from the theoretical values were lower than 5%. The method described has a potential application to in vitro penetration studies of CysA using porcine skin as a biological membrane model.

Cited by 1 publication

References 22 publications

Assessment of in vitro methodologies to determine topical and transdermal delivery of the flavonoid quercetin

Assessment of in vitro methodologies to determine topical and transdermal delivery of the flavonoid quercetin

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