Fos-like immunoreactivity in central nervous system of mice simultaneously exposed to the elevated plus-maze and nociception

Gomes, Keila Rejane Oliveira; Garcia, Cristiane Álvares; Planeta, Cleópatra da Silva; Nunes-de-Souza, Ricardo Luiz

doi:10.1590/s1516-93322005000300012

Cited by 3 publications

(3 citation statements)

References 27 publications

(34 reference statements)

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“…it provoked a reduction in Fos-positive labeled cells in the amygdaloid complex, strongly suggesting that the increased Fos expression in oEPM-exposed mice was not simply due to novelty. It is important to highlight that the currently employed eEPM habituation protocol was necessary in order to minimize the potential confound of acute handling stress that has been reported to be crucial to reduce per se effects on the basal number of Fospositive cells [29,47]. However, habituation is stressor specific, such that decreased c-fos response to a familiar stressor, does not prevent induction of this gene by a novel stressor [48].…”

Section: Discussionmentioning

confidence: 99%

“…The protein Fos has been the most commonly used IEG marker of neuronal activity in behavioral research, including studies on fear and anxiety [24]. For instance, increased Fos expression has been identified in the amygdala of animals exposed to a conventional EPM [25][26][27][28][29]. Together, this body of evidence suggests that the amygdala might play a role in the modulation of defensive reactions displayed by mice during the exposure to the oEPM.…”

Section: Introductionmentioning

confidence: 99%

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Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

Sorregotti

Cipriano

Cruz

et al. 2018

Behavioural Brain Research

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Previous studies have shown that the exposure to an open elevated plus maze (oEPM, an EPM with all four open arms) elicits fear/anxiety-related responses in laboratory rodents. However, very little is known about the underlying neural substrates of these defensive behaviors. Accordingly, the present study investigated the effects of chemical inactivation of the amygdala [through local injection of cobalt chloride (CoCl: a nonspecific synaptic blocker)] on the behavior of oEPM-exposed mice. In a second experiment, the pattern of activation of the basolateral (BLA) and central (CeA) nuclei of the amygdala was assessed through quantification of Fos protein expression in mice subjected to one of several behavioral manipulations. To avoid the confound of acute handling stress, 4 independent groups of mice were habituated daily for 10days to an enclosed EPM (eEPM) and, on day 11 prior to immunohistochemistry, were either taken directly from their home cage (control) or individually exposed for 10min to a new clean holding cage (novelty), an eEPM, or the oEPM. An additional group of mice (maze-naïve) was not subjected to either the habituation or exposure phase but were simply chosen at random from their home cages to undergo an identical immunohistochemistry procedure. Results showed that amygdala inactivation produced an anxiolytic-like profile comprising reductions in time spent in the proximal portions of the open arms and total stretched attend postures (SAP) as well as increases in time spent in the distal portions of the open arms and total head-dipping. Moreover, Fos-positive labeled cells were bilaterally increased in the amygdaloid complex, particularly in the BLA, of oEPM-exposed animals compared to all other groups. These results suggest that the amygdala (in particular, its BLA nucleus) plays a key role in the modulation of defensive behaviors in oEPM-exposed mice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

Sorregotti

Cipriano

Cruz

et al. 2018

Behavioural Brain Research

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“…mice and rats) exhibit in an aversive situation, elicited by open arms. The level of anxiety is evaluated by open arm avoidance (entries and time in open arms), while general activity is evaluated by the frequency of closed arm entries 21 …”

Section: Discussionmentioning

confidence: 99%

The management of menopause with complementary and alternative medicine using an experimental model: Ovariectomized rats

Bello¹,

Batestusso²,

Oliveira

et al. 2010

J of Obstet and Gynaecol

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TESTE DE CONSTRIÇÃO ABDOMINAL NÃO APRESENTA EFEITO SOBRE O COMPORTAMENTO EXPLORATÓRIO DE CAMUNDONGOS ALBINOS (Mus musculus) NO LABIRINTO EM CRUZ ELEVADO

Silva¹,

Alvarenga²,

Oliveira³

2014

RUVRV

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Resumo: Apesar da realização de vários trabalhos sobre os efeitos antinoceptivos do confinamento de camundongos nos braços abertos do labirinto em cruz elevado (LCE), poucos estudos investigaram o efeito da estimulação nociceptiva sobre o comportamento exploratório propriamente dito. Além, não há consenso na literatura acerca da influência da estimulação nociceptiva sobre o comportamento exploratório do camundongo no LCE. Portanto, o objetivo deste trabalho foi investigar o efeito do teste de constrição abdominal sobre o comportamento exploratório de camundongos albinos no LCE. Camundongos albinos (Mus musculus) adultos foram distribuídos em dois grupos: ACA (n=05) e SAL (n=05). No grupo ACA, os animais foram tratados com 0,05 ml/kg de ácido acético 0,6% i.p. No grupo SAL, os animais receberam injeção i.p. de salina. Uma hora depois, os animais foram expostos, individualmente, ao LCE por cinco minutos. Registrou-se o tempo de permanência nos braços abertos do LCE. Não foi observada diferença estatisticamente significativa (t=-0,21999, p<0,001) na duração média dos tempos de permanência nos compartimentos do LCE entre os animais dos dois grupos. . Devido à influência ativadora da dor sobre a resposta fisiológica ao estresse, esperava-se que os sujeitos do grupo ACA permanecessem menos tempo nos braços abertos do LCE do que os do grupo SAL. Os presentes resultados indicaram que a dor resultante do teste de constrição abdominal parece não influenciar no comportamento exploratório de camundongos no LCE.

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Fos-like immunoreactivity in central nervous system of mice simultaneously exposed to the elevated plus-maze and nociception

Cited by 3 publications

References 27 publications

Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

The management of menopause with complementary and alternative medicine using an experimental model: Ovariectomized rats

TESTE DE CONSTRIÇÃO ABDOMINAL NÃO APRESENTA EFEITO SOBRE O COMPORTAMENTO EXPLORATÓRIO DE CAMUNDONGOS ALBINOS (Mus musculus) NO LABIRINTO EM CRUZ ELEVADO

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