Handbook of food enzymology

Filho, Flavio Finardi

doi:10.1590/s1516-93322004000200020

Cited by 3 publications

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“…LOX can trigger the hydrolysis reaction of oil and grease to produce glycerol molecules and fatty acid molecules, and the small molecules of free fatty acid produced therein have a serious sour and rotten smell, which directly affects the stability and storage quality of rice bran. Nowadays, it is usually judged whether the remaining enzymes in rice bran are inactivated by whether the enzyme activity of oxidase is lower than 4U—as the main basis for judging the stability of rice bran (Finardi Filho, 2004). And the LOX activity of rice bran after extrusion is lower than 4U, LOX activity is obviously reduced, which means rice bran acidification is inhibited and stability is improved, so injecting CO 2 and extruding is beneficial to the stabilization of rice bran.…”

Section: Resultsmentioning

confidence: 99%

Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

2022

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Background and Objectives Rice bran is rich in nutrients but not very stable and available, so extrusion of it broadens its utilization value. The objective was to investigate the effects of extrusion parameter variables on the physicochemical properties and lipoxygenase activity of extruded rice bran. The extrusion parameter variables were set as extrusion temperature (80, 90, 110, 140°C); CO2 injection, and no CO2 injection; screw speed (200, 300 rpm). Findings CO2 injection significantly affected the expansion characteristics and lipoxygenase activity of extruded rice bran. It better inhibits lipoxygenase activity and at a fixed screw speed of 200 rpm, the bulk density of extruded rice bran with CO2 injection was higher than that of conventional extrusion. After extrusion, the soluble dietary fiber content was significantly increased in the extruded samples compared with the raw material. In contrast, the content of dietary fiber was not significantly affected by CO2 injection. Conclusions Compared with the conventional extrusion method, the CO2‐injected extrusion has significant effects on the expansion characteristics and nutritional composition. Extrusion with CO2 injection can reduce lipoxygenase activity more effectively. Significance and Novelty The results prove that CO2 extrusion can inhibit the lipoxygenase activity, and change the physicochemical properties of rice bran compared with conventional extrusion. It provides a basis for the application of CO2 as a medium in extruded rice bran food and a new process for processing rice bran by‐products.

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Section: Resultsmentioning

confidence: 99%

Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

2022

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Production of Industrial Enzymes Based on the Expression System of the Fungus Penicillium verruculosum

Sinitsyn

Синицына

Рожкова

2021

Appl Biochem Microbiol

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Production of Industrial Enzymes based on the Expression System of the Penicillium verruculosum Fungus

Синицына

Рожкова

2020

Biiotehnologiâ (Mosk.)

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The review provides the data on the creation of highly active recombinant strains producers of a wide range of enzymes for various fields of industry and agriculture using the expression system of the Penicillium verruculosum filamentous fungus: endoglucanases, β-glucanases, xylanases, mannanases, phytases, α-galactosidases, pectin lyases, polygalacturonases, dextranases, exo-inulinases and chitinases. The recipient strain, P. verruculosum B1-537(ΔniaD), has a high secretory capacity (up to 60 g/L of extracellular protein in the culture liquid), and the recombinant producer strains are characterized by high productivity of target enzymes. The obtained enzyme preparations contain, as a rule, 30--70% of the target recombinant enzymes (in some cases, up to 80%) of the total protein pool. The P. verruculosum expression system makes it easy to transform expression constructs containing target heterologous or homologous genes, functionally linked to the promoter and terminator of the strong inducible promoter of the cellobiohydrolase-1 (cbh1) gene, and the genetic transformation of the recipient strain can be carried out simultaneously using several vector constructs. The incorporation of target genes into the chromosome of the recipient strain is an integrative process, and the foreign recombinant proteins encoded by them are not subjected to proteolysis. The technique of primary selection of positive transformants on agar media, as well as secondary selection during their cultivation in shaking flasks and fermenters, is simple and reliable in use, partly owing to standardized parameters: the nutrient composition of the fermentation medium and the culturing conditions of recombinant strains producing various target enzymes. The time spent on creating a producer of the target enzyme, from setting the problem to obtaining a recombinant strain, takes from 3 to 6 months. It is important to emphasize that recombinant P. verruculosum strains are successfully used in industrial processes as producers of various target enzymes. expression system, cloning, industrial enzymes, feed additives, Penicillium verruculosum The work was supported by the Ministry of Science and Higher Education of the Russian Federation (State Assignment 0104-2019-0009).

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Handbook of food enzymology

Cited by 3 publications

References 0 publications

Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

Production of Industrial Enzymes Based on the Expression System of the Fungus Penicillium verruculosum

Production of Industrial Enzymes based on the Expression System of the Penicillium verruculosum Fungus

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Handbook of food enzymology

Cited by 3 publications

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Effect of high‐pressure CO2 injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

Effect of high‐pressure CO2 injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

Production of Industrial Enzymes Based on the Expression System of the Fungus Penicillium verruculosum

Production of Industrial Enzymes based on the Expression System of the Penicillium verruculosum Fungus

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Product

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Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran

Effect of high‐pressure CO₂ injection on the physicochemical properties and lipoxygenase activity of extruded rice bran